Interferences in clinical chemistry analysis

Abstract

Analyses of body fluids in clinical chemistry laboratory are subject to a number of interferences that affect the analytical accuracy. The interferents arise from exogenous sources like drugs and additives as well as such endogenous sources like lipemia, hemolysis and icteria. Our studies demonstrate matrix interference in the form of analytical bias between serum and aqueous matrix calibrators. The clinical chemist should constantly be aware of this factor. Correction of interferences is recommended as an integral part of the quality assurance program.

Clinical Biochemistry and Hematology

“…The increased turbidity associated with lipemia can produce artificially high values for certain enzymes. Lipemia also interferes with the assays for glucose, bilirubin, total protein, phosphorus, and urates (Saibaba et al, 1998). Hemolysis also produces errors in clinical biochemical values because most serum enzymes are present at high concentrations in red blood cells.…”

Effect of Lipemia on Glucose Oxidase Method

“…'nın yaptığı ça-lışmada lipit konsantrasyonu arttıkça elde edilmesi gereken sonuçtan yüzde olarak ne kadar uzaklaşıldı-ğını gösteren diyagram Şekil 3'te gösterilmiştir. [16] Sonuç olarak, heksokinaz yöntemi referans alın-dığında kullanılan Siemens Advia 2400 sistemi için glukoz oksidaz kit prospektüsünde normal glukoz ve trigliserit düzeyleri için verilen yüksek interferans oranını destekleyen bir veriye ulaşamadık. Üretici firma tarafından intralipid solüsyonları ile bildirilen lipemi interferansı sonuçları değerlendirilirken endojen lipemi interferansını yansıtmayabileceği dikkate alınmalıdır.…”

Deceased tissue donor serology and molecular testing for HIV, hepatitis B and hepatitis C viruses: a lack of cadaveric validated tests

“…The Cobas System Ò have their own cadaveric sample diluent (Cobas Ò TaqScreen Cadaveric Speciment Diluent) (Roche Ò 2015a), instead of Procleix Ò Ultrio Ò which suggests in-house protocol for the dilution (1:5 in saline: 0.9 % sodium chloride, i.e., 100 lL sample plus 400 lL saline). The Cobas Ò TaqScreen MPX (Roche Ò 2015a) is the only assay kit that provides a list of possible endogenous and exogenous interferent analites, including a strong advise about Heparin, even though no other manufacters (Hologic and FioCruz) specify this polysaccharide as an interferent (Bio-Manguinhos Ò FioCruz 2015; Hologic Ò 2012).…”