Interferência da condição climática na integridade de espermatozoides ovinos submetidos à criopreservação

Silva, Sildivane Valcácia; Soares, Adriana Trindade; Batista, André Mariano; Almeida, Felipe Costa; Guerra, Maria Madalena Pessoa

doi:10.1590/s0102-09352011000600005

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…These authors have also reviewed the importance of evaluating the indicators of reproductive function such as fertility, prolificacy, reproductive behavior, body condition score, maternal ability, and survival rate of the offsprings in order to determine the adaptability of the genetic groups to their environment. It is important to point out that the influence of climatic factors on the production and reproduction of livestock has been a subject of great interest in recent years with research aiming to understand its particularities and thus improve animal production, as demonstrated by Moura et al (2014), Oliveira et al (2013a), and Silva et al (2011).…”

Section: Introductionmentioning

confidence: 99%

Reproductive and productive performances of Santa Inês ewes submitted to breeding in different periods of the Amazonian humid tropical climate

Soares

Oliveira

Padilha-Nakaghi

et al. 2015

Trop Anim Health Prod

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The objective of this study was to evaluate the reproductive and productive performance of Santa Inês ewes bred at different times of the year in humid tropical climate. One hundred and forty-eight Santa Inês ewes were grouped according to the time of the year of their breeding season (i.e., mating period) (dry/wet, wet, wet/dry, and dry season). The service type was natural mating and the ewes and rams were kept together every night for 45 days. Reproductive efficiency was assessed by service, pregnancy, lambing, prolificacy, twinning, pregnancy loss, weaning, and lamb mortality rates. Ewes were weighed at the beginning and at the end of the breeding season and before and after parturition, and sequential weighing of the lambs was performed (at birth, 15, 30, 60, and 90 days). Reproductive efficiency index (number of lambs weaned/total of served ewes) and productive efficiency (kg of weaned lamb/kg of served or lambed ewes) were calculated. All ewes expressed estrus early in the breeding season; however, a higher percentage (53.5 and 7.1 % at 30 and 45 days, respectively) of ewes returned to estrus during the wet/dry period. The lower rates (13.9 %) of return to estrus at 30 days were during the wet season (P < 0.05). There were no (P > 0.05) effects of breeding seasons on the remaining reproductive rates. Ewes that lambed during the wet/dry transition period weighted less, before (40.5 ± 2.5 kg) and after (38.6 ± 1.6 kg) parturition, than those of other groups (P < 0.05). Lamb weight at birth did not vary between groups, however, weight at weaning was higher (15.6 ± 2.1 kg) in lambs born during the wet season (P < 0.05). The reproductive efficiency index was lower (0.66) when the breeding season took place during the dry/wet period (P < 0.05). Productive rates were significantly higher (0.29 and 0.33 for kg of weaned lamb/kg of served and lambed ewes, respectively; P < 0.05) in ewes served in the dry season. The reproductive performance of Santa Inês ewes was not significantly influenced by the period of the year in which the breeding seasons took place, allowing for four breeding seasons a year in the Amazon region. Variations between periods in return to estrus rates, weight of ewes close to parturition and lamb weight at weaning indicate that climate changes can also affect reproductive rates.

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Section: Introductionmentioning

confidence: 99%

Reproductive and productive performances of Santa Inês ewes submitted to breeding in different periods of the Amazonian humid tropical climate

Soares

Oliveira

Padilha-Nakaghi

et al. 2015

Trop Anim Health Prod

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“…The effect of seasonality on sperm quality for Santa Inês rams is well known, as is the effect on semen freezability (SILVA et al, 2011a).…”

Section: Resultsmentioning

confidence: 99%

Effect of catalase, superoxide dismutase and reduced glutathione in LDL extender on ovine cryopreserved sperm viability

et al. 2015

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The aim of this study was to evaluate the motility, kinetics and membrane integrity of ovine sperm cryopreserved in extenders containing 8% LDL with enzymatic antioxidants at different concentrations. Four Santa Inês rams were used to form four pools of semen (each pool containing ejaculates from four ram, totaling four ejaculates per animal). Each seminal pool was divided into eight aliquots for the following treatments: 1) Tris-glucose-glycerol (TGG) + (16%) egg yolk (control 1); 2) TGG + 8% (w/v) LDL (control 2); 3) TGG + 8% LDL + catalase 100 U/mL; 4) TGG + 8% LDL + catalase 200 U/mL; 5) TGG + 8% LDL + superoxide dismutase 100 U/mL; 6) TGG + 8% LDL + superoxide dismutase 200 U/mL; 7) TGG + 8% LDL + reduced glutathione 5 mM; and 8) TGG + 8% LDL + reduced glutathione 10 mM. The samples were packed into 0.25 mL straws, cooled (-0.25 °C/ min), maintained at 5 °C for 2 h and then frozen (-25 °C/ min) using a TK4000 ® . Immediately after thawing (38 °C/ 30 s), sperm motility and movement characteristics were assessed by computer sperm analysis (CASA). The structural integrity of the plasma and acrosomal membranes was analyzed using fluorescent dyes. The functional integrity of membranes was assessed using a hypoosmotic swelling test. As assessed by ANOVA, significant differences (P<0.05) among treatments were only observed for VCL, VSL and VAP. For the VCL variable, the 2, 3, 4, 5, 6 and 7 extenders were similar and higher than 1 and 8 extenders, the latter being similar to each other. For the VSL variable, the 3, 4, 5, 6 and 7 extenders were similar and higher than 1, 2 and 8 extenders, the latter being similar to each other. For the VAP variable, the 3, 4 and 6 extenders were similar and higher than 1, 2, 5, 7 and 8 extenders, the latter being similar to each other. In conclusion, enzymatic antioxidants as catalase and superoxide dismutase improve the protective activity of extenders containing LDL on frozen ovine sperm. concentrações. Quatro carneiros da raça Santa Inês foram utilizados para formar quatro pools de sêmen (cada pool contendo ejaculados provenientes dos quatro carneiros, totalizando quatro ejaculados por animal). Cada pool de sêmen foi dividido em oito alíquotas para os seguintes tratamentos: 1) Trisglicose-glicerol (TGG) + (16%) gema de ovo (controle 1); 2) TGG + 8% (g/L) LDL (controle 2); 3) TGG + 8% LDL + catalase 100 U/mL; 4) TGG + 8% LDL + catalase 200 U/mL; 5) TGG + 8% LDL + superóxido dismutase 100 U/mL; 6) TGG + 8% LDL + superóxido dismutase 200 U/mL; 7) TGG + 8% LDL + glutationa reduzida 5 mM; and 8) TGG + 8% LDL + glutationa reduzida 10 mM. As amostras foram envasadas em palhetas de 0,25 mL, resfriadas (-0,25 °C/min), mantidas a 5 °C por duas horas e em seguida congeladas (-25 °C/ min) usando uma máquina de congelar TK4000 ® . Imediatamente depois da descongelação (38 °C/30 s), as amostras foram submetidas à análise computadorizada (CASA) para avaliação da motilidade e cinética. A integridade estrutural das membranas plasmática e acrossomal foi analisada utilizando corantes fluor...

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Melatonin membrane receptors MT1 and MT2 are expressed in ram spermatozoa from non-seasonal breeds

Carvajal‐Serna

Neira-Rivera

Cardozo

et al. 2020

Trop Anim Health Prod

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Interferência da condição climática na integridade de espermatozoides ovinos submetidos à criopreservação

Cited by 4 publications

References 12 publications

Reproductive and productive performances of Santa Inês ewes submitted to breeding in different periods of the Amazonian humid tropical climate

Reproductive and productive performances of Santa Inês ewes submitted to breeding in different periods of the Amazonian humid tropical climate

Effect of catalase, superoxide dismutase and reduced glutathione in LDL extender on ovine cryopreserved sperm viability

Melatonin membrane receptors MT1 and MT2 are expressed in ram spermatozoa from non-seasonal breeds

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