Interfering with the Actin Network and Its Effect on Long-Term Potentiation and Synaptic Tagging in Hippocampal CA1 Neurons in Slices<i>In Vitro</i>

Ramachandran, Binu; Frey, Julietta U.

doi:10.1523/jneurosci.2045-09.2009

Cited by 120 publications

(99 citation statements)

References 39 publications

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“…This inconsistency may be ascribed to the different features of actin dynamics caused by different LTP-inducing stimuli. A stable but increased level of actin polymerization may be essential for the maintenance of LTP induced by PKC activation (28,59). At least two distinct roles of F-actin polymerization have been proposed for LTP-inducing synaptic stimulation (18).…”

Section: Discussionmentioning

confidence: 99%

“…NMDAR combined with autophosphorylated CaMKII translocates to the postsynaptic membrane following exposure to LTP-inducing stimuli, including PKC activation and burst stimulation (TBS) (7). Importantly, this NMDARCaMKII complex is proposed to serve as a "seed" for anchoring other plasticity-related proteins, including AMPA receptors (AMPARs) (28,29). We thus hypothesize that the myosin IIbdependent regulation of actin dynamics is crucial for NMDAR trafficking during synaptic plasticity.…”

Section: N-methyl-d-aspartate Receptor (Nmdar) Synaptic Incorporationmentioning

confidence: 99%

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Myosin IIb-dependent Regulation of Actin Dynamics Is Required for N-Methyl-d-aspartate Receptor Trafficking during Synaptic Plasticity

Wang

et al. 2015

Journal of Biological Chemistry

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Background:The motor protein myosin IIb has been detected in the N-methyl-D-aspartate receptor (NMDAR)-associated protein complex. Results: Myosin IIb is essential for NMDAR synaptic incorporation during synaptic plasticity. Conclusion:The myosin light chain kinase (MLCK)-and myosin IIb-dependent regulation of actin dynamics is required for NMDAR trafficking during synaptic plasticity. Significance: This study provides new insight into how the actin cytoskeleton underpins NMDAR plasticity.

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Section: Discussionmentioning

confidence: 99%

Section: N-methyl-d-aspartate Receptor (Nmdar) Synaptic Incorporationmentioning

confidence: 99%

Myosin IIb-dependent Regulation of Actin Dynamics Is Required for N-Methyl-d-aspartate Receptor Trafficking during Synaptic Plasticity

Wang

et al. 2015

Journal of Biological Chemistry

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“…As suggested elsewhere (Sajikumar et al, 2005), it is possible that levels of memoryrelated proteins decrease with incubation time, reflecting their normal half lives and ongoing proteolytic activity. Under these depleted conditions, TBS could initiate changes in the actin cytoskeleton needed for rapid consolidation and perhaps also initiate the synthesis of proteins needed to sustain the potentiated state (Kelly et al, 2007;Ramachandran and Frey, 2009). To be clear, according to this account, relevant proteins are depleted during a prolonged incubation of slices creating a condition in which the production of enduring LTP will depend on proteins synthesized in response to afferent stimulation.…”

Section: Incubation Time Determines the Effect Of Protein Synthesis Imentioning

confidence: 99%

Protein synthesis and consolidation of memory-related synaptic changes

2015

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Published findings and new results described here challenge this idea. Protein synthesis inhibitors did not prevent Theta Bust Stimulation (TBS) from producing extremely stable longterm potentiation (LTP) in experiments using standard hippocampal slice protocols. However, the inhibitors were effective under conditions that likely depleted protein levels prior to attempts to induce the potentiation effect. Experiments showed that induction of LTP at one input, and thus a prior episode of protein synthesis, eliminated the effects of inhibitors on potentiation of a second input even in depleted slices. These observations suggest that a primary role of translation and transcription processes initiated by learning events is to prepare neurons to support future learning. Other work has provided support for an alternative theory of consolidation. Specifically, if the synaptic changes that support memory are to endure, learning events/TBS must engage a complex set of signaling processes that reorganize and re-stabilize the spine actin cytoskeleton. This is accomplished in fast (10 min) and slow (50 min) stages with the first requiring integrin activation and the second a recovery of integrin functioning. These results align with, and provide mechanisms for, the long-held view that memories are established and consolidated over a set of temporally distinct phases.

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“…One possible function of the rapid recruitment of synaptic components is to serve as such a tag for synapse-specific longterm growth (8). Consistent with that idea, actin polymerization, which is important for rapid changes in synaptic puncta and structures (14,16,17,(28)(29)(30)(31), is also known to be important for synaptic tagging (47).…”

Section: Discussionmentioning

confidence: 74%

Rapid increase in clusters of synaptophysin at onset of homosynaptic potentiation in Aplysia

Jin

Udo

Hawkins

2011

Proc. Natl. Acad. Sci. U.S.A.

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Imaging studies have shown that even the earliest phases of longterm plasticity are accompanied by the rapid recruitment of synaptic components, which generally requires actin polymerization and may be one of the first steps in a program that can lead to the formation of new stable synapses during late-phase plasticity. However, most of those results come from studies of long-term potentiation in rodent hippocampus and might not generalize to other forms of synaptic plasticity or plasticity in other brain areas and species. For example, recruitment of presynaptic proteins during long-term facilitation by 5HT in Aplysia is delayed for several hours, suggesting that whereas activity-dependent forms of plasticity, such as long-term potentiation, involve rapid recruitment of presynaptic proteins, neuromodulatory forms of plasticity, such as facilitation by 5HT, involve more delayed recruitment. To begin to explore this hypothesis, we examined an activity-dependent form of plasticity, homosynaptic potentiation produced by tetanic stimulation of the presynaptic neuron in Aplysia. We found that homosynaptic potentiation involves presynaptic but not postsynaptic actin and a rapid (under 10 min) increase in the number of clusters of the presynaptic vesicle-associated protein synaptophysin. These results indicate that rapid recruitment of synaptic components is not limited to hippocampal potentiation and support the hypothesis that activity-dependent types of plasticity involve rapid recruitment of presynaptic proteins, whereas neuromodulatory types of plasticity involve more delayed recruitment.synapse assembly | synaptic tagging | sensory neuron | motor neuron | cell culture W hereas short-term plasticity involves covalent modifications of existing synapses, long-term plasticity often involves the formation of new synapses (1-8). A good deal is now known about synapse assembly during development (9, 10), but much less is known about the rules, sequence, and mechanisms of synapse assembly during long-term plasticity. Imaging studies have shown that even the earliest phases of long-term plasticity are accompanied by the rapid (within minutes) recruitment of synaptic components (11-16). The initial recruitment of synaptic components generally requires actin polymerization but not protein synthesis, whereas the long-term maintenance of these components requires protein synthesis (7,8,14,(16)(17)(18). These findings suggest that the early recruitment of synaptic components may be some of the first steps in a program that can lead to the protein synthesis-dependent formation of new stable synapses during late-phase plasticity.Most of these results come from studies of long-term potentiation (LTP) in rodent hippocampus, however, and whether other forms of synaptic plasticity and plasticity in other brain areas and species are accompanied by a similar rapid recruitment of synaptic proteins is not known. For example, recruitment of presynaptic proteins during long-term facilitation by 5HT in Aplysia is delayed for several hours ...

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Interfering with the Actin Network and Its Effect on Long-Term Potentiation and Synaptic Tagging in Hippocampal CA1 Neurons in SlicesIn Vitro

Cited by 120 publications

References 39 publications

Myosin IIb-dependent Regulation of Actin Dynamics Is Required for N-Methyl-d-aspartate Receptor Trafficking during Synaptic Plasticity

Myosin IIb-dependent Regulation of Actin Dynamics Is Required for N-Methyl-d-aspartate Receptor Trafficking during Synaptic Plasticity

Protein synthesis and consolidation of memory-related synaptic changes

Rapid increase in clusters of synaptophysin at onset of homosynaptic potentiation in Aplysia

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