The interferometric nanoprofilometer (INP), developed at the Institute of Process Measurement and Sensor Technology at the Ilmenau University of Technology, is a precision device for measuring the profile deviations of plane mirrors with a profile length of up to 250 mm at the nanometre scale. As its expanded uncertainty of U(l) = 7.8 nm at a confidence level of p = 95% (k = 2) was mainly influenced by the uncertainty of the straightness standard (3.6 nm) and the uncertainty caused by the signal and demodulation errors of the interferometer signals (1.2 nm), these two sources of uncertainty have been the subject of recent analyses and modifications. To measure the profile deviation of the standard mirror we performed a classic three-flat test using the INP. The three-flat test consists of a combination of measurements between three different test flats. The shape deviations of the three flats can then be determined by applying a least-squares solution of the resulting equation system. The results of this three-flat test showed surprisingly good consistency, enabling us to correct this systematic error in profile deviation measurements and reducing the uncertainty component of the standard mirror to 0.4 nm. Another area of research is the signal and demodulation error arising during the interpretation of the interferometer signals. In the case of the interferometric nanoprofilometer, the special challenge is that the maximum path length differences are too small during the scan of the entire profile deviation over perfectly aligned 250 mm long mirrors for proper interpolation and correction since they do not yet cover even half of an interference fringe. By applying a simple method of weighting to the interferometer data the common ellipse fitting could be performed successfully and the demodulation error was greatly reduced. The remaining uncertainty component is less than 0.5 nm. In summary we were successful in greatly reducing two major systematic errors. The interferometric nanoprofilometer in this machine generation is a precision device for laboratory use, able to measure profile deviations of 250 mm long profiles with an expanded combined standard uncertainty of 2.5 nm at a confidence level of p = 95% (k = 2).