Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events

Sancho-Fornes, Gabriel; Avella-Oliver, Miquel; Carrascosa, Javier; Puchades, Rosa; Maquieira, Ángel

doi:10.1016/j.snb.2020.129289

Cited by 3 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The large Stokes shift, the distinctive emission spectrum, the distinctive size and shape of the lanthanide complex aggregates, and the unique chemical bonds of the constituent molecules can be recognized by combining fluorescence microscopy and PiFM characterization through layers of protein. The OEu 3+ Phen and Eu 3+ (TTA) 3 Phen complexes showed red fluorescence, which could be observed with a transmission illumination (365 nm) fluorescence microscope through protein layers. The oleic acid carboxylate groups of the OEu 3+ Phen complexes were identified by PiFM despite an intervening protein layer.…”

Section: Prognosis For Biometric Recognition Applicationsmentioning

confidence: 92%

“…Using an XSP-63XD fluorescence microscope with~365 nm illumination, the sample had strong red fluorescence, demonstrating that Eu 3+ complexes (OEu 3+ Phen and Eu(TTA) 3 Phen complexes) could be detected through the protein coating layer as shown in Figure 4f. The addition of the Eu(TTA) 3 Phen layer was only intended to improve the fluorescence intensity of the sample. As can be seen from Figure 4c, the addition of Eu 3+ (TTA) 3 Phen layer did not prevent the observation of nanostructures that are consistent with the size of with size of OEu 3+ Phen complexes observed via TEM.…”

Section: The Effect Of Different Thicknesses Of Protein Layersmentioning

confidence: 99%

“…As can be seen from Figure 4c, the addition of Eu 3+ (TTA) 3 Phen layer did not prevent the observation of nanostructures that are consistent with the size of with size of OEu 3+ Phen complexes observed via TEM. Therefore, the Eu(TTA) 3 Phen complex will aid rather than hinder biorecognition.…”

Section: The Effect Of Different Thicknesses Of Protein Layersmentioning

confidence: 99%

“…To realize this purpose, the available materials will be most important. As a general strategy for designing the materials that are embedded under skin and interact with biological tissues, nanomaterials have increasing potential to match the needs of this area [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Stable Fluorescence of Eu3+ Complex Nanostructures Beneath a Protein Skin for Potential Biometric Recognition

et al. 2021

View full text Add to dashboard Cite

We designed and realized highly fluorescent nanostructures composed of Eu3+ complexes under a protein coating. The nanostructured material, confirmed by photo-induced force microscopy (PiFM), includes a bottom fluorescent layer and an upper protein layer. The bottom fluorescent layer includes Eu3+ that is coordinated by 1,10-phenanthroline (Phen) and oleic acid (O). The complete complexes (OEu3+Phen) formed higher-order structures with diameter 40–150 nm. Distinctive nanoscale striations reminiscent of fingerprints were observed with a high-resolution transmission electron microscope (HRTEM). Stable fluorescence was increased by the addition of Eu3+ coordinated by Phen and 2-thenoyltrifluoroacetone (TTA), and confirmed by fluorescence spectroscopy. A satisfactory result was the observation of red Eu3+ complex emission through a protein coating layer with a fluorescence microscope. Lanthanide nanostructures of these types might ultimately prove useful for biometric applications in the context of human and non-human tissues. The significant innovations of this work include: (1) the structural set-up of the fluorescence image embedded under protein “skin”; and (2) dual confirmations of nanotopography and unique nanofingerprints under PiFM and under TEM, respectively.

show abstract

Section: Prognosis For Biometric Recognition Applicationsmentioning

confidence: 92%

Section: The Effect Of Different Thicknesses Of Protein Layersmentioning

confidence: 99%

Section: The Effect Of Different Thicknesses Of Protein Layersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Stable Fluorescence of Eu3+ Complex Nanostructures Beneath a Protein Skin for Potential Biometric Recognition

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The mapping of the diffraction efficiency along the whole area was performed with a custom scanning system that sequentially moves the surface and collects the optical signals, as described elsewhere. 41 Two photosensors were incorporated in this case to measure the transmitted zeroth and first orders ( Figure S6 ), and RSD values were calculated from the diffraction efficiency of all the pixels within the sensing area (20 × 1.2 mm). The resulting data from the scans were smoothed with a Savitzky–Golay filter (second-order polynomial, 30 points).…”

Section: Methodsmentioning

confidence: 99%

Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing

Juste-Dolz

Delgado-Pinar

Avella-Oliver

et al. 2022

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

The nanostructuration of biolayers has become a paradigm for exploiting nanoscopic light-matter phenomena for biosensing, among other biomedical purposes. In this work, we present a photopatterning method to create periodic structures of biomacromolecules, based on a local and periodic mild denaturation of protein biolayers mediated by UV-laser irradiation. These nanostructures are constituted by a periodic modulation of the protein activity, so they are free of topographic and compositional changes along the pattern. Herein we introduce the approach, explore the patterning parameters, characterize the resulting structures, and assess their overall homogeneity. This UV-based patterning principle has proven to be an easy, cost-effective, and fast way to fabricate large areas of homogeneous one-dimensional protein patterns (2 min, 15 x 1.2 mm, relative standard deviation ≃ 16%). This work also investigates the implementation of these protein patterns as transducers for 2 diffractive biosensing. Using a model immunoassay, these patterns have demonstrated negligible signal contributions from non-specific bindings and comparable experimental limits of detection in buffer medium and in human serum (53 and 36 ng•mL -1 of unlabelled IgG, respectively).

show abstract

A label-free biosensor based on E-SMS optical fiber structure for anti BSA detection

Patiño-Jurado

Cardona-Maya

Jaramillo-Grajales

et al. 2022

Optical Fiber Technology

View full text Add to dashboard Cite

Interferometric multilayered nanomaterials for imaging unlabeled biorecognition events

Cited by 3 publications

References 40 publications

Stable Fluorescence of Eu3+ Complex Nanostructures Beneath a Protein Skin for Potential Biometric Recognition

Stable Fluorescence of Eu3+ Complex Nanostructures Beneath a Protein Skin for Potential Biometric Recognition

Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing

A label-free biosensor based on E-SMS optical fiber structure for anti BSA detection

Contact Info

Product

Resources

About