Interferón en la hepatitis B

A mammalian expression vector comprised of the PreS2-TLM (translocation motif), a single-chain variable fragment (ScFv) that binds to hepatitis B surface antigen (HBsAg) and the EGFP gene was constructed. A stably transformed cell line that could express and secrete the fusion protein (PreS2-TLM-ScFv-EGFP) was established. HBsAg-positive HepG2.2.15 cells and HepG2 and HeLa cells were incubated with the supernatant of the transformed cell line cultures for evaluating the cellular permeability of PreS2-TLM-ScFv-EGFP. The location of the fusion protein PreS2-TLM-ScFv-EGFP in HepG2.2.15 cells was observed with immunofluorescence staining. EGFP was next replaced by a dominant negative mutant of the hepatitis B virus core gene (HBcDN) for producing fusion protein PreS2-TLM-ScFv-HBcDN, which was detected by western blot. The supernatant containing fusion protein PreS2-TLM-ScFv-HBcDN was added to the cultures of HepG2.2.15 cells, and the packaged hepatitis B virus (HBV) pregenomic RNA expression levels in the cells were measured using qRT-PCR. The results of the in vitro study indicated that the packaged HBV pregenomic RNA expression levels in HepG2.2.15 cells significantly decreased when these cells were exposed to the supernatant at the dose of 25% for 24, 48 and 72 h, or at the dose of 12.5% for 72 h.

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Therapeutic effects of interferons in human viral infections

Barrales-Cureño

Reyes

Diaz-Bautista

et al. 2018

mexjbiotechnol

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The interferon (IFN) is a cytokine produced by immunocompetent cells in response to various stimuli. Five types of IFNs are identified: alpha, beta, gamma, tau and omega 1. In particular, interferons inhibit viral replication directly by antiviral mechanisms, as they do so indirectly by amplifying immune responses to viral proteins. Likewise, they are also essential elements in clinical oncology. They are used in the treatment of chronic myelocytic and hairy cell leukemia, multiple myeloma, non-Hodgkin's lymphoma, melanoma, renal carcinoma, and Kaposi's sarcoma, as well as in the papilloma. They are also used in diseases of viral origin such as hepatitis B and C, acute respiratory syndrome, and HIV (experimentally in the latter), as well as in neurodegenerative diseases, including

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Interferón en la hepatitis B

Cited by 3 publications

References 69 publications

Targeting the Achilles heel of the hepatitis B virus: a review of current treatments against covalently closed circular DNA

Targeting the Achilles heel of the hepatitis B virus: a review of current treatments against covalently closed circular DNA

Inhibition effect produced by dominant negative mutant fusion protein PreS2‐TLM‐ScFv‐HBcDN on HBV replication in vitro

Therapeutic effects of interferons in human viral infections

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