Interferon-?? Induces a Decrease in the Susceptibility of Human Glioma Cells to Lysis by Lymphokine-activated Killer Cells

Yin, Dali; Kondo, Seiji; Takeuchi, Juji; Morimura, Tatsuo; Nakatsu, Shouji; Oda, Yoshifumi; Kikuchi, Hiroe

doi:10.1097/00006123-199407000-00017

Cited by 2 publications

(1 citation statement)

References 24 publications

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“…42 IFN␥ was shown previously to induce resistance to NK-mediated lysis in other tumor types; in some cases this was ascribed to enhanced MHC class I expression, particularly when unmodified tumor cells expressed low levels of MHC class I molecules. 37,38,62,63 Since RCC-26 cells constitutively express substantial levels of HLA class I molecules, IFN␥ induced only minor increases in class I expression on occasion. Whether alterations in the expression of particular allotypes account for the induced resistance to lysis by non-MHC-restricted T cells remains to be determined.…”

Section: Discussionmentioning

confidence: 99%

Gene transfer of human interferon gamma complementary DNA into a renal cell carcinoma line enhances MHC-restricted cytotoxic T lymphocyte recognition but suppresses non-MHC-restricted effector cell activity

et al. 2000

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Even though renal cell carcinomas (RCC) are thought to be immunogenic, many tumors express variations in surface molecules and intracellular proteins that hinder induction of optimal antitumor responses. Interferon gamma (IFN␥) stimulation can correct some of these deficiencies. Therefore, we introduced the complementary DNA (cDNA) encoding human IFN␥ into a well-characterized RCC line that has been selected for development of an allogeneic tumor cell vaccine for treatment of patients with metastatic disease. Studies were performed to determine how endogenous IFN␥ expression influences tumor cell immunogenicity. IFN␥ transductants showed minimal increases in surface expression of MHC class I and adhesion molecules but

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Section: Discussionmentioning

confidence: 99%

Gene transfer of human interferon gamma complementary DNA into a renal cell carcinoma line enhances MHC-restricted cytotoxic T lymphocyte recognition but suppresses non-MHC-restricted effector cell activity

et al. 2000

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Effects of IFN-γand Interleukin-1βon Major Histocompatibility Complex Antigen and Intercellular Adhesion Molecule-1 Expression by 9L Gliosarcoma: Relevance to Its Cytolysis by Alloreactive Cytotoxic T Lymphocytes

Schiltz

Gomez

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et al. 2002

Journal of Interferon & Cytokine Research

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To enhance the efficacy of cellular immunotherapy for gliomas, we tested the concept of using proinflammatory cytokine treatment with interferon-gamma (IFN-gamma) or interleukin-1beta (IL-1beta) or both to render glioma cells more susceptible to cytolysis by alloreactive cytotoxic T lymphocytes (aCTL). The cytokines, separately or in combination, were able to upregulate major histocompatibility complex (MHC) class I antigen or intercellular adhesion molecule-1 (ICAM-1) on Fischer rat 9L gliosarcoma cells. 9L cells were incubated in vitro for 24, 48, or 72 h with varying concentrations of rat IFN-gamma (0-2000 U/ml) or recombinant human IL-1 (rHUIL-1) (0-1000 U/ml) or both. By 48 h, IFN-gamma (500 U/ml) maximally induced the percentage of positive expressing cells and the relative antigen density of MHC class I and ICAM-1 on 9L cells, whereas IL-1 induced only ICAM-1 expression. Simultaneous incubation of IL-1 with IFN-gamma did not further affect the induction of class I on 9L cells more than that achieved with IFN-gamma alone. 9L cells with upregulated MHC class I and ICAM-1 expression were more sensitive to lysis by aCTL in in vitro cytotoxicity assays, regardless of whether the precursor aCTL came from naive or from 9L-immunized rats. Furthermore, inhibition of 9L cytotoxicity in assays that included blocking antibodies to MHC class I or to ICAM-1 revealed that T cell receptor (TCR) interactions with MHC class I and that ICAM-1 interactions with lymphocyte function-associated-1 (LFA-1) antigen account for a portion of the glioma lysis by aCTL.

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Interferon-?? Induces a Decrease in the Susceptibility of Human Glioma Cells to Lysis by Lymphokine-activated Killer Cells

Cited by 2 publications

References 24 publications

Gene transfer of human interferon gamma complementary DNA into a renal cell carcinoma line enhances MHC-restricted cytotoxic T lymphocyte recognition but suppresses non-MHC-restricted effector cell activity

Gene transfer of human interferon gamma complementary DNA into a renal cell carcinoma line enhances MHC-restricted cytotoxic T lymphocyte recognition but suppresses non-MHC-restricted effector cell activity

Effects of IFN-γand Interleukin-1βon Major Histocompatibility Complex Antigen and Intercellular Adhesion Molecule-1 Expression by 9L Gliosarcoma: Relevance to Its Cytolysis by Alloreactive Cytotoxic T Lymphocytes

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