Interferon Induction by Viruses and Polynucleotides: A Differential Effect of Camptothecin

Atherton, K. T.; Burke, D. C.

doi:10.1099/0022-1317-29-3-297

Cited by 38 publications

(13 citation statements)

References 14 publications

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“…Lymphoblastoid and fibroblast interferons were prepared from Namalva and HEF-9 cells by published techniques (Atherton & Burke, 1975;Strander et al I975). Partially purified human leucocyte interferon was a kind gift from Professor K. Cantell.…”

Section: Differences In Sialic Acid Content Of Human Interferonsmentioning

confidence: 99%

Differences in Sialic Acid Content of Human Interferons

Morser¹,

Kabayo

Hutchinson

1978

Journal of General Virology

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Section: Differences In Sialic Acid Content Of Human Interferonsmentioning

confidence: 99%

Differences in Sialic Acid Content of Human Interferons

Morser¹,

Kabayo

Hutchinson

1978

Journal of General Virology

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“…Interferon was assayed by the RNA-reduction method (Atherton & Burke, 1975) with HFF cells for human fibroblast interferon and BT cells for lymphoblastoid interferon. The results are expressed in reference units, based on the human leukocyte interferon research standard (69/19), which is defined as containing 5ooo reference research units and which has a titre of/5oo in HFF cells and 5oooo in BT cells.…”

Section: Methodsmentioning

confidence: 99%

The Effect of 5-Bromodeoxyuridine on Interferon Production in Human Cells

Baker

Bradshaw

Morser

et al. 1979

Journal of General Virology

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SUMMARY5-Bromodeoxyuridine (BrdUrd) increased interferon production by the Namalwa line of human lymphoblastoid cells treated with Sendai virus, but inhibited their growth. Thymidine, which also inhibited cell growth had no effect on interferon production, so that growth inhibition per se was not the cause of the stimulation. BrdUrd was incorporated into cellular DNA; 5-chlorodeoxyuridine and 5-iododeoxyuridine (which are also incorporated) increased the interferon yield, but 5-fluorodeoxyuridine (which is not incorporated) did not. Thymidine reduced both the incorporation of BrdUrd and its stimulatory effect on interferon production. Deoxycytidine (which prevents the cytotoxic effects of BrdUrd) had no effect on the stimulation. BrdUrd also stimulated interferon production in response to poly(rI).poly(rC) in growing human diploid fibroblasts but not in SV4o virus-transformed human cells. Since BrdUrd was incorporated into the DNA of all these cells, we concluded that incorporation is necessary, but not sufficient for the stimulation of interferon formation.

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“…Samples for interferon assays were dialysed for 5 days at 4 °C against pH 2 buffer to inactivate residual virus. Interferon assays were carried out in human foreskin fibroblasts (HFF) (Atherton & Burke, 1975). Interferon titres are expressed as international units (IU): the interferon-fl international reference preparation (G-023 902 527) containing 10000 IU/ml, titrated at 5000 U/ml in HFF.…”

Section: Discussionmentioning

confidence: 99%