Interferon-tau stimulated gene expression: A proxy to predict embryonic mortality in dairy cows

Sheikh, Aasif Ahmad; Hooda, O. K.; Kalyan, Ankita; Kamboj, Aarti; Mohammed, Seid; Alhussien, Mohanned Naif; Reddi, Srinu; Shimray, Panreiphy Gachuiwo; Rautela, Ankita; Pandita, Sujata; Kapila, Suman; De, Sachinandan; Dang, Ajay Kumar

doi:10.1016/j.theriogenology.2018.07.028

Cited by 34 publications

(33 citation statements)

References 46 publications

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“…Similar to previous studies, the mRNA expression of ISGs in the white blood cells was higher in pregnant animals than in non-pregnant animals (Fig. 2) [26,27,28,29,30,31]. Importantly, the co-transfer of bovine trophoblast vesicles with IVF embryos increases the fertility of demi-embryos during the early stages of pregnancy, suggesting that adding trophoblasts (vesicles or embryo) appear to have a potential role in maternal pregnancy recognition via enhanced IFNT secretion [32, 33].…”

Section: Discussionsupporting

confidence: 89%

Improvement of fertility in repeat breeder dairy cattle by embryo transfer following artificial insemination: possibility of interferon tau replenishment effect

Yaginuma¹,

Funeshima

Tanikawa

et al. 2019

J. Reprod. Dev.

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Repeat breeder cattle do not become pregnant until after three or more breeding attempts; this represents a critical reproductive disorder. Embryo transfer (ET) following artificial insemination (AI) in repeat breeder cattle reportedly improves pregnancy rate, leading to speculation that interferon tau (IFNT) is associated with this phenomenon. However, the reason why the conception rate improves remains unknown. We investigated the effect of ET following AI on repeat breeder cattle in field tests, and determined whether adding an embryo affects the maternal immune cells detected by interferon-stimulated genes (ISGs), marker genes of IFN response. In total, 1122 repeat breeder cattle were implanted with in vitro fertilization (IVF) embryos after previous AI. ET following AI resulted in pregnancy rates of 46.9% in repeat breeder dairy cattle. In basic in vivo tests, to investigate the effect of adding embryos, ISGs mRNA expression levels were significantly higher in the AI + ET group than in the AI + sham group (transfer of only embryonic cryopreservation solution). Then, we examined the effect of cultured conditioned media (CM) of IVF embryos on splenic immune cells and Madin-Darby bovine kidney (MDBK) cells with stably introduced ISG15 promoter-reporter constructs. These cells exhibited a specific increase in ISG15 mRNA expression and promoter activity when treated with the CM of IVF embryos, suggesting that IVF embryos have the potential to produce and release IFNT. In conclusion, ET following AI is beneficial for improving conception in repeat breeder cattle. Added embryos may produce and secrete IFNT, resulting in the increased expression of ISGs.

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Section: Discussionsupporting

confidence: 89%

Improvement of fertility in repeat breeder dairy cattle by embryo transfer following artificial insemination: possibility of interferon tau replenishment effect

Yaginuma¹,

Funeshima

Tanikawa

et al. 2019

J. Reprod. Dev.

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“…and MX1 mRNA in blood neutrophils in pregnant and late embryonic mortality cows than that in early embryonic mortality cows, which indicates that upregulation of OAS1 and MX1 in blood neutrophils is necessary for embryonic survival during early pregnancy in cattle (Sheikh et al, 2018). Therefore, this finding supported the idea that early pregnancy induces upregulation of OAS1 and MX1 in maternal liver, which may be beneficial for normal pregnancy in sheep.…”

Section: Discussionsupporting

confidence: 67%

Effects of early pregnancy on expression of interferon‐stimulated gene 15, STAT1, OAS1, MX1, and IP‐10 in ovine liver

Yang

Zhang

et al. 2020

Animal Science Journal

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Interferon-tau (IFNT) regulates maternal recognition during early pregnancy in ruminants. The liver can serve as a hematopoietic organ, and it has immune functions. This study hypothesized whether mRNA and proteins of interferon-stimulated genes (ISGs) induced by early pregnancy are upregulated in maternal liver. Therefore, we determined the expression of interferon-stimulated gene 15-kDa protein (ISG15), 2',5'-oligoadenylate synthetase 1 (OAS1), myxovirus resistance protein 1 (MX1), interferon-gamma-inducible protein 10 (IP-10), and signal transducer and activator of transcription 1 (STAT1) in maternal livers during early pregnancy in sheep. Ovine livers were sampled on day 16 of the estrous cycle, and days 13, 16, and 25 of pregnancy, and expression of ISGs was detected by quantitative real-time PCR, Western blot, and immunohistochemistry analysis. Our results showed that there were increases in expression of the mRNA and proteins of ISG15, OAS1, IP-10, STAT1, and MX1 during early pregnancy. STAT1 protein was limited to the hepatocytes, and endothelial cells of proper hepatic arteries and hepatic portal veins. In conclusion, the upregulation of ISG15, OAS1, IP-10, STAT1, and MX1 proteins may be implicated in maternal hepatic immune adjustment and other functions during early pregnancy in sheep.

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“…In addition to their function as predictors of pregnancy, ISG expression could reflect embryonic viability since there is a positive correlation between the concentrations of IFNT released by the conceptus and the abundance of ISG ( Matsuyama et al., 2012 ). Some studies ( Matsuyama et al., 2012 ; Sheikh et al., 2018 ) reported different ISG expression patterns between females who maintained pregnancy and females who experienced a pregnancy loss, suggesting that these differences could be explored in the prediction of embryonic or fetal mortality, while others found no differences ( Shirasuna et al., 2015 ; Melo et al., 2020a ). Because the events leading to embryonic or fetal mortality are not always related to the development and expansion of the trophectoderm, ISG expression patterns may detect some but not all cases of pregnancy loss.…”

Section: Chemical-based Methods: Non-pregnancy Specificmentioning

confidence: 99%

New approaches to diagnose and target reproductive failure in cattle

et al. 2020

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Reproductive failure and pregnancy loss in cattle are some of the largest economic burdens to cattle producers and one of most perplexing factors influencing management decisions. Pregnancy loss may occur at any point during gestation with the largest percentage of loss occurring in the first 30 days and, subsequently, decreasing as the pregnancy progresses. Losses may be attributed to numerous factors, predisposed issues or environmental conditions such as nutritional stressors or disease. From a research perspective, determining the exact causes of pregnancy loss or embryonic mortality in cattle have been difficult, due to limitations of accurately determining early gestation pregnancy status. Until methods that precisely determine embryo success early in gestation are available, our understanding of in vivo pregnancy loss will lack clarity necessary to develop management strategies to decrease such loss. In this review, we will briefly discuss the pivotal periods of pregnancy loss affecting beef and dairy cattle, methods and technologies to determine pregnancy status and embryo viability and potential opportunities to decrease reproductive failure.

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Interferon-tau stimulated gene expression: A proxy to predict embryonic mortality in dairy cows

Cited by 34 publications

References 46 publications

Improvement of fertility in repeat breeder dairy cattle by embryo transfer following artificial insemination: possibility of interferon tau replenishment effect

Improvement of fertility in repeat breeder dairy cattle by embryo transfer following artificial insemination: possibility of interferon tau replenishment effect

Effects of early pregnancy on expression of interferon‐stimulated gene 15, STAT1, OAS1, MX1, and IP‐10 in ovine liver

New approaches to diagnose and target reproductive failure in cattle

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