1997
DOI: 10.1074/jbc.272.16.10797
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Interferon-γ Activation of a Mitogen-activated Protein Kinase, KFR1, in the Bloodstream Form of Trypanosoma brucei

Abstract: KFR1, a mitogen-activated protein (MAP) kinase identified in the African trypanosome, Trypanosoma brucei, is a serine protein kinase capable of phosphorylating the serine residues in histone H-1, myelin basic protein, and ␤-casein. It phosphorylates four proteins with estimated molecular masses of 22, 34, 46, and 90 kDa from the T. brucei bloodstream-form lysate in vitro. KFR1 bears significant sequence similarity to the yeast MAP kinases KSS1 and FUS3 but cannot functionally complement the kss1/fus3 yeast mut… Show more

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Cited by 37 publications
(24 citation statements)
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“…Invasion of T. brucei brucei was also inhibited in RAG-1 -/-mice, suggesting that B and/or T cells facilitate migration of T. brucei brucei into the brain and that innate immune cell-derived IFN-γ is not sufficient for T. brucei brucei penetration through the BBB. IFN-γ may have direct effects on T. brucei brucei (25,26). However, a similar pattern of T. brucei brucei neuroinvasion was observed in IFN-γ -/-and IFN-γR -/-mice, indicating that IFN-γ facilitates T. brucei brucei migration into the brain parenchyma by acting on the tissues of the host rather than by altering the phenotype of the parasites.…”
Section: Discussionmentioning
confidence: 73%
“…Invasion of T. brucei brucei was also inhibited in RAG-1 -/-mice, suggesting that B and/or T cells facilitate migration of T. brucei brucei into the brain and that innate immune cell-derived IFN-γ is not sufficient for T. brucei brucei penetration through the BBB. IFN-γ may have direct effects on T. brucei brucei (25,26). However, a similar pattern of T. brucei brucei neuroinvasion was observed in IFN-γ -/-and IFN-γR -/-mice, indicating that IFN-γ facilitates T. brucei brucei migration into the brain parenchyma by acting on the tissues of the host rather than by altering the phenotype of the parasites.…”
Section: Discussionmentioning
confidence: 73%
“…BLAST searches of the T. brucei genome database (www.ebi.ac.uk /parasites/parasite-genome.html) with sequences from MAP kinases from plants allowed the identification of nine MAP kinase homologues in T. brucei, three of which have been described previously (9,18,26). One of the kinases, TbMAPK5 (accession number Tb927.6.4220), which has not yet been investigated, contains a TDY sequence motif in the activation domain.…”
Section: Resultsmentioning
confidence: 99%
“…Domains with known functions, including the ATP binding site and a domain diagnostic for serine/threonine kinases (S/T), are overlined. (B) Radial phylogenetic tree of the MAP kinases described above, together with MPK3 (MP_114433) from Arabidopsis thaliana, KSS1 (M26398) from yeast, ERK5 (Q13164) and JNK2␣1 (AAC50606) from humans, SAPK␣ (P92208) from Drosophila melanogaster, p38 (Q9Z1B7) from mice, KFR1 (18) and MAPK2 (26) from T. brucei, and MAPK1 (NC_702183) and MAPK2 (JC5153) from Plasmodium falciparum were constructed with the TreeView software (http: //taxonomy.zoology.gla.ac.uk/rod/rod.html). Bootstrap values indicating the number of occurrences of branch points during 1,000 replications are given for the three primary branches consisting of the ERK subgroup, stress-activated protein kinases, and MAP kinases from protozoa.…”
Section: Fig 1 Tbmapk5 Is Related To Map Kinases (A)mentioning
confidence: 99%
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“…KFR1 is likely involved in proliferation of the bloodstream form and is regulated by gamma interferon, an extracellular molecule (20). TbMAPK2 is required for differentiation of the bloodstream form to the procyclic form and for subsequent cell proliferation (21).…”
mentioning
confidence: 99%