Interferon-γ preferentially reduces memory/effector CD8 T lymphocytes in healthy subjects

Interferon‐γ in healthy subjects: selective modulation of inflammatory mediators

“…IFN-g administration did not cause chills, nausea, or other signs of acute illness. During the control study, IFN-g levels remained around the detection limit of the assay (31 pg mL 21 ). In the intervention study, IFN-g levels increased gradually to 518^96 pg mL 21 after 6 h, P , 0´05 vs. control).…”

“…Neutrophilic expression of FcgRI (CD64, high-affinity Fc receptor for immunoglobulin G) was measured using flow cytometry (at t 30 min, and 4, 8 and 24 h) as described previously [21]. Neutrophilic expression of FcgRI (CD64, high-affinity Fc receptor for immunoglobulin G) was measured using flow cytometry (at t 30 min, and 4, 8 and 24 h) as described previously [21].…”

“…These assays were performed according to the instructions of the manufacturers. C-reactive protein (CRP) and secretory phospholipase A2 (sPLA2) levels were measured using ELISAs as previously described by Wolbink et al [17,18] with detection limits of 0´15 mg mL 21 and 1 ng mL 21 . Plasma concentrations of neutrophilic elastase complexed to a 1 -antitrypsin (further referred to as elastase) and lactoferrin were measured by specific radioimmunoassays (RIA) as previously described, both with a detection limit of 20 ng mL 21 [19].…”

“…Neutrophil counts were determined by flow cytometry (Technicon H1 system, Technicon Instruments, Tarrytown, NY, USA). Neutrophilic expression of FcgRI (CD64, high-affinity Fc receptor for immunoglobulin G) was measured using flow cytometry (at t 30 min, and 4, 8 and 24 h) as described previously [21]. Cells were IFN-g-induced inflammatory mediators 537 incubated with anti-CD64 mAbs directly labelled with fluorescein isothiocyanate (Medarex, Annandale, NJ).…”

Targeting Immunotherapy to the Tumor Microenvironment

“…Systemic IL-12 in humans leads to dose-limiting toxicities including lymphopenias and elevated transaminases that can be fatal [Del Vecchio et al, 2007]. IFNg is slightly better tolerated than IL-12, but leads to severe lymphopenias, and a compensatory production of indoleamine 2,3-dioxygenase (IDO) [de Metz et al, 1999]. IFNa produces response rates of 5-30% in patients with locally advanced melanoma, but is not well-tolerated [Nicholas and Lesinski, 2011].…”