Interferon-γ Release Assay Performance for Tuberculosis in Childhood

Kay, Alexander; Islam, Shamim; Wendorf, Kristen; Westenhouse, Janice; Barry, Pennan M.

doi:10.1542/peds.2017-3918

Cited by 57 publications

(43 citation statements)

References 35 publications

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“…In this study, the rate of indeterminate test results for children was even lower (2.2%) and was significantly lower than that for adults (5.2%). A recent study by Kay and colleagues reported an overall rate of indeterminate IGRA results of 3.7% (12 of 322 children) and found that 4 of 5 children Ͻ2 years of age with indeterminate IGRA results had central nervous system (CNS) disease, suggesting that CNS disease may be a stronger driver of indeterminate test results than age (33). In our population, only 1 of 4 children with CNS disease had an indeterminate QFT-IT result.…”

Section: Discussionmentioning

confidence: 48%

QuantiFERON-TB Performs Better in Children, Including Infants, than in Adults with Active Tuberculosis: a Multicenter Study

Lombardi

Pellegrino²,

Denicolò

et al. 2019

J Clin Microbiol

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Immunological tests, including the QuantiFERON-TB Gold In-Tube (QFT-IT) assay, represent an important aid for diagnosing active tuberculosis (TB) and latent TB infections in children, but concerns about their use in children <5 years of age persist. This is a multicenter retrospective study comparing a population of 226 children to 521 adults with pulmonary or extrapulmonary TB. The aim was to evaluate the QFT-IT performance, analyzing both qualitative and quantitative results, according to age, birthplace, and disease localization. Compared to culture, QFT-IT sensitivity was 93.9%, 100%, and 94.4% in children ≤2, 2 to 5, and 5 to 16 years of age, respectively, and was significantly higher than that in adults (81.0%) (P < 0.0001). The rate of indeterminate test results for children (2.2%) was significantly lower than that for adults (5.2%) (P < 0.0001). In children, QFT-IT sensitivity was not affected by disease localization or birthplace (Italy born versus foreign born). Interferon gamma (IFN-γ) values in response to TB antigen and mitogen were significantly higher in children than in adults (TB antigen, median of 10 versus 1.66 IU IFN-γ/ml; mitogen, median of 10 versus 6.70 IU IFN-γ/ml; P < 0.0001). In summary, this study supports the use of QFT-IT as a complementary test for the diagnosis of pediatric TB even under 2 years of age. Our observations could be applicable to the new version of the test, QuantiFERON-TB Gold Plus, which has recently been shown to have similar sensitivity in active TB, although data in children are still lacking.

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Section: Discussionmentioning

confidence: 48%

QuantiFERON-TB Performs Better in Children, Including Infants, than in Adults with Active Tuberculosis: a Multicenter Study

Lombardi

Pellegrino²,

Denicolò

et al. 2019

J Clin Microbiol

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“…Interestingly, the IGRA sensitivity is reduced compared to TST in children younger than 2 years old. [16] Furthermore, IGRA seems to offer alternative LTBI detection, especially babies living with TB parents. [17] This study has shown that the newborns were still negative for IGRA 12 weeks after BCG vaccination, suggesting that BCG may stimulate immune response and play a protective role against TB disease.…”

Section: Discussionmentioning

confidence: 99%

Interferon Gamma Release Assay After BCG Vaccination Among Newborns and Family Members Living in an Overcrowded Area in Jakarta, Indonesia

Tanoerahar

Rooslamiati

Kipuw

et al. 2020

Preprint

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IntroductionActive tuberculosis (TB) patient is a potential source of Mycobacterium tuberculosis transmission in an overcrowded and poor area. Family members living in the same house may have been infected or latent tuberculosis infection (LTBI) may develop. The aim of the study was to explore LTBI among newborns and their family members living in an overcrowded area in Jakarta, Indonesia. MethodsA prospective analytical study was conducted among newborns from October 2016 to March 2017. Interferon gamma release assay (IGRA) was examined before BCG vaccination and after 12 weeks. In parallel, TB active case finding was performed among family members of the newborns.ResultsOf 135 newborns, only 117 (86.7%) came for BCG vaccination. Of 346 family members screened, 8 (2.3%) were detected as untreated active pulmonary TB, confirmed by positive sputum and/or MTB culture. Family members living in the same house with active TB individuals (p=0.011, OR 2.69) as well as being males (p = 0.025; OR 1.68) had a significant higher risk of having a positive IGRA. ConclusionsUntreated pulmonary TB infection in overcrowded areas infects the surrounding neighbors, resulting in latent TB infection. An active program for detecting pulmonary TB cases and preventive measures need to be taken seriously to contain the potential spreading of the infection.

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“…IFN‐γ ELISA has been developed to assess CMI in TB and is one of the IFN‐γ IGRAs used for latent tuberculosis infection diagnosis . Although the clinical performance of IGRAs varies according to the characteristics of each study, it is superior to that of the tuberculin skin test in most cases . Furthermore, IFN‐γ ELISA has already been applied for VZV CMI assessment and is a rapid and simple quantitative method with high specificity and sensitivity for direct IFN‐γ detection by using whole blood rather than peripheral blood mononuclear cells.…”

Section: Discussionmentioning

confidence: 99%

“…26,[40][41][42] Although the clinical performance of IGRAs varies according to the characteristics of each study, it is superior to that of the tuberculin skin test in most cases. [43][44][45][46][47] Furthermore, IFN-γ ELISA has already been applied for VZV CMI assessment 21,24,25,32 and is a rapid and simple quantitative method with high specificity and sensitivity for direct IFN-γ detection by using whole blood rather than peripheral blood mononuclear cells. In the present study, VZVspecific CMI in response to a live attenuated HZ vaccine in individuals was assessed by an IFN-γ ELISA.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Varicella‐zoster virus‐specific cell‐mediated immunity by interferon‐γ Enzyme‐Linked Immunosorbent Assay in adults ≥50 years of age administered a herpes zoster vaccine

Yang

Chen

Zhang³

et al. 2019

Journal of Medical Virology

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Varicella‐zoster virus (VZV)‐specific cell‐mediated immunity (CMI) is critical for preventing and controlling the onset of herpes zoster (HZ). To assess VZV CMI, an interferon‐γ (IFN‐γ) enzyme‐linked immunosorbent assay (ELISA) was validated by examining the influence of VZV‐specific antigen content, incubation time, and interval from whole blood collection on the assay. In phase II clinical trial, VZV‐specific CMI in adults ≥50 years of age administered an HZ vaccine were evaluated by IFN‐γ ELISA, as determined by measuring IFN‐γ production in the whole blood in response to stimulation with ultraviolet light‐inactivated VZV. The VZV‐specific IFN‐γ levels varied among individuals from prevaccination (baseline) to 6 weeks postvaccination. In most subjects, VZV‐specific CMI was increased at 6 weeks postvaccination. The HZ vaccine elicited a significant increase in the VZV‐specific CMI response as measured by ELISA; the geometric mean fold‐rises from baseline to 6 weeks postvaccination were 3.50, 4.22, and 5.24 in the 4.3, 4.7, and 4.9 log plaque‐forming unit vaccine groups, respectively, which was significantly higher than in the placebo group (P < 0.05). These results indicate that vaccination enhances the VZV‐specific CMI responses in subjects; IFN‐γ ELISA is an effective method for evaluating the CMI response and may be useful for identifying individuals at a high risk of HZ infection.

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Interferon-γ Release Assay Performance for Tuberculosis in Childhood

Cited by 57 publications

References 35 publications

QuantiFERON-TB Performs Better in Children, Including Infants, than in Adults with Active Tuberculosis: a Multicenter Study

QuantiFERON-TB Performs Better in Children, Including Infants, than in Adults with Active Tuberculosis: a Multicenter Study

Interferon Gamma Release Assay After BCG Vaccination Among Newborns and Family Members Living in an Overcrowded Area in Jakarta, Indonesia

Evaluation of Varicella‐zoster virus‐specific cell‐mediated immunity by interferon‐γ Enzyme‐Linked Immunosorbent Assay in adults ≥50 years of age administered a herpes zoster vaccine

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