Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells1

Okuda, Kiyoshi; Kasahara, Yuko; Murakami, Shuko; Takahashi, Hitomi; Wocławek-Potocka, Izabela; Skarzynski, Dariusz J.

doi:10.1095/biolreprod.103.019083

Cited by 27 publications

(23 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The RT-PCRs were conducted with the housekeeping gene ACTB as an internal standard. ACTB primer was added at the appropriate cycle number by the "primer-drop-ping method" as described by Wong et al [23] with our own modification [24]. The PCRs were carried out using TaKaRa Taq (R001A; Takara Bio, Shiga, Japan) and a thermal [25], using BSA as a standard.…”

Section: Reverse Transcription-pcrmentioning

confidence: 99%

Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F2.ALPHA. Production in Bovine Luteal Endothelial Cells

Lee

Acosta

Nakagawa

et al. 2010

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

Abstract. Prostaglandin F2α (PGF) induces a rapid reduction in progesterone production (functional luteolysis) followed by tissue degeneration and cell death (structural luteolysis). Reactive oxygen species (ROS) including nitric oxide (NO) play crucial roles in the luteolytic action of PGF. The local concentration of intraluteal ROS is controlled by superoxide dismutase (SOD), the main enzyme involved in the control of intraluteal ROS. To clarify the roles of NO in the regulation of SOD in luteolysis, we examined the effects of NO on SOD expression and activity in cultured bovine luteal endothelial cells (LECs) during short-term (2 h, mimicking functional luteolysis) and long-term (24 h, mimicking structural luteolysis) incubation. We also investigated whether NO modulates PGF production by LECs. LECs were isolated from mid-luteal phase CLs, and exposed to NONOate (a NO donor) for 2 or 24 h. SOD mRNA expression was stimulated by NONOate (10-100 μM) at 2 h (P<0.05). Moreover, 10 μM NONOate stimulated SOD protein expression and SOD activity at 2 h (P<0.05), whereas NONOate inhibited SOD mRNA and protein expressions at 24 h (P<0.05). NONOate stimulated PGF biosynthesis at both incubation times. The overall findings suggest that NO differently regulates SOD in cultured LECs, depending on the exposure time. Acute elevation of SOD may represent a response of LECs to protect themselves against oxidative stress induced by PGF during functional luteolysis, whereas a later reduction of SOD levels by NO may facilitate an excess of intraluteal ROS during structural luteolysis. Key words: Luteal endothelial cell, Nitric oxide, Superoxide dismutase (J. Reprod. Dev. 56: [454][455][456][457][458][459] 2010) he corpus luteum (CL) is a transient endocrine gland essential for the regulation of ovarian cycles as well as for establishment of pregnancy in mammals. If pregnancy does not occur, the CL regresses and loses its capacity to produce progesterone (P4), so the animal returns to estrus and has another opportunity to become pregnant. Regression of the CL (luteolysis) is crucial to reset the ovarian cycle. Prostaglandin F2α (PGF) produced by the uterus induces a rapid reduction in P4 production (functional luteolysis) followed by tissue degeneration and cell death (structural luteolysis) [1][2][3]. Although PGF is regarded as a physiological luteolysin in the cow, the local mechanism involved in the luteolytic action of PGF remains unclear.The CL formed from the wall of the ovulated follicle is one of the most highly vascularized organs in the body, and luteal endothelial cells (LECs) are the most abundant type of cells composing the CL [4]. Previous studies have demonstrated that endothelial cells produce reactive oxygen species (ROS) [5] including nitric oxide (NO) [6,7] and that ROS inhibit P4 production [8,9] and induce apoptosis in bovine luteal steroidogenic cells (LSCs) [10,11]. Therefore, ROS including NO in the LEC may play an important role for elucidating the local mechanisms of functional and structural luteolysi...

show abstract

Section: Reverse Transcription-pcrmentioning

confidence: 99%

Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F2.ALPHA. Production in Bovine Luteal Endothelial Cells

Lee

Acosta

Nakagawa

et al. 2010

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

show abstract

“…Semiquantitative RT-PCR was carried out using the housekeeping gene G3PDH as an internal standard. G3PDH primer was added at the appropriate cycle number by the ''primer-dropping method'' as described by Wong et al [23] with our own modification [24]. The primers for G3PDH, which were designed as described by Tsai et al [25], were 5Ј-TGT TCC AGT ATG ATT CCA CCC-3Ј (5Ј primer, 21 mer) and 5Ј-TCC ACC ACC CTG TTG CTG TA-3Ј (3Ј primer, 20 mer).…”

Section: Experiments 4 Effect Of a Specific P4 Antagonist And Fas L Omentioning

confidence: 99%

Progesterone Is a Suppressor of Apoptosis in Bovine Luteal Cells1

Okuda

Korzekwa

Shibaya

et al. 2004

Biology of Reproduction

Self Cite

114

View full text Add to dashboard Cite

Progesterone is suggested to be a suppressor of apoptosis in bovine luteal cells. Fas antigen (Fas) is a cell surface receptor that triggers apoptosis in sensitive cells. Furthermore, apoptosis is known to be controlled by the bcl-2 gene/protein family and caspases. This study was undertaken to determine whether intraluteal progesterone (P4) is involved in Fas L-mediated luteal cell death in the bovine corpus luteum (CL) in vitro. Moreover, we studied whether an antagonist of P4 influences gene expression of the bcl-2 family and caspase-3 and the activity of caspase-3 in the bovine CL. Luteal cells obtained from the cows in the midluteal phase of the estrous cycle (Days 8-12 of the cycle) were exposed to a specific P4 antagonist (onapristone [OP], 10(-4) M) with or without 100 ng/ml Fas L. Although Fas L alone did not show a cytotoxic effect, treatment of the cells with OP alone or in combination with Fas L resulted in killing of 30% and 45% of the cells, respectively (P <0.05). DNA fragmentation was observed in the cells treated with Fas L in the presence of OP. The inhibition of P4 action by OP increased the expression of Fas mRNA (P <0.01); however, it did not affect bax or bcl-2 mRNA expression (P >0.05). Moreover, OP stimulated expression of caspase-3 mRNA (P <0.01). The overall results indirectly show that intraluteal P4 suppresses apoptosis in bovine luteal cells through the inhibition of Fas and caspase-3 mRNA expression and inhibition of caspase-3 activation.

show abstract

“…The antiluteolytic effects of IFN-τ include inhibition of endometrial estrogen receptor mRNA expression, thereby inhibiting expression of oxytocin receptor and production of PGF2α pulses [26][27][28]. Additionally, IFN-τ suppresses TNFα-induced PGF2α by decreasing COX2 gene expression in the stromal cells of the bovine endometrium [29]. These findings suggest that the TVs we transferred may have developed until an adequate time and secreted IFN-τ, after which IFN-τ inhibited the uterine PGF2α in the heifers with a prolonged cycle.…”

Section: Discussionmentioning

confidence: 99%

Production of Trophoblastic Vesicles Derived From Day 7 and 8 Blastocysts of In Vitro Origin and the Effect of Intrauterine Transfer on the Interestrous Intervals in Japanese Black Heifers

Nagai

Sata

Takahashi

et al. 2009

Journal of Reproduction and Development

Self Cite

View full text Add to dashboard Cite

Abstract. This study was undertaken to produce trophoblastic vesicles (TVs) by using blastocysts of in vitro origin and to estimate the effect on the interestrous interval after transfer of 4 TVs into the uteri of heifers on Day 7. Morphological examination under a stereoscopic microscope revealed that the total formation rate of TVs prepared from IVP expanded blastocysts was 80.5% and that there was no difference in the formation rates of TVs derived from blastocysts between Day 7 (83.5%) and Day 8 (78.5%). After intrauterine transfer of TVs, observation of the corpus luteum (CL) by transrectal ultrasonography together with measurement of the plasma progesterone concentration confirmed that 2 of 4 recipients (50%) had a longer interestrous interval, 33.5 and 35.0 days, while the other 2 recipients had normal cycles, 20.0 and 24.5 days. In the control group transferred D-PBS, all 4 heifers had a normal cycles, 24.0-24.5 days. Consequently, the average number of days after intrauterine transfer of TVs compared with the 2 consecutive cycles just before the treatment was longer than in the controls (6.1 ± 2.4 days vs. -0.8 ± 1.1 days, P<0.05). These results indicate that preparation of TVs from blastocysts of in vitro origin is a useable method and that TVs from blastocysts may have the capacity to maintain CL function after intrauterine transfer. Key words: Blastocyst, Corpus luteum, Estrous cycle, Trophoblastic vesicle (J. Reprod. Dev. 55: [454][455][456][457][458][459] 2009) mbryo transfer makes it possible to obtain more than one offspring per year from valuable cattle. In Japan, 80% of embryo transfers are conducted using frozen-thawed embryos [1]. However, the pregnancy rate of frozen-thawed embryo transfer is still low (45%) compared with that of fresh embryo transfer (50%) [2]. Improving fertility is necessary because of the efficiency of frozenthawed embryo transfer for control of reproduction and breeding in cattle.In ruminants, Interferon-tau (IFN-τ) is recognized as the pregnancy recognition signal [3] to maintain progesterone (P4) secretion from the corpus luteum (CL) during the pre-implantation period. The CL is a prerequisite for establishing pregnancy [4]. IFN-τ is secreted from the embryonic trophectoderm, which will develop into the main part of the future placenta [3,5]. Previously, it has been reported that the pregnancy rates following transfer of biopsied fresh, vitrified and frozen embryos are 50, 44 and 23%, respectively [6]. The pregnancy rate may tend to decrease since the level of manipulation for storage of the embryos may produce inadequate IFN-τ as result of by damage to the embryonic trophectoderm. In addition, up to 40% of total embryonic losses occur between Day 7 and 17 of pregnancy [7]. The bovine conceptus must secrete the highest amount of IFN-τ as signal for maternal recognition pregnancy between Day 15 and 17 [8]. Thus, less IFN-τ may be one reason for early embryonic loss.A prolonged cycle and delay of luteolysis has been confirmed by injection of homogenates of Day 17-18 ...

show abstract

Interferon-τ Blocks the Stimulatory Effect of Tumor Necrosis Factor-α on Prostaglandin F2α Synthesis by Bovine Endometrial Stromal Cells1

Cited by 27 publications

References 50 publications

Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F2.ALPHA. Production in Bovine Luteal Endothelial Cells

Role of Nitric Oxide in the Regulation of Superoxide Dismutase and Prostaglandin F2.ALPHA. Production in Bovine Luteal Endothelial Cells

Progesterone Is a Suppressor of Apoptosis in Bovine Luteal Cells1

Production of Trophoblastic Vesicles Derived From Day 7 and 8 Blastocysts of In Vitro Origin and the Effect of Intrauterine Transfer on the Interestrous Intervals in Japanese Black Heifers

Contact Info

Product

Resources

About