Intergeneric bacterial coaggregations involving mutans streptococci and oral actinomyces

Crowley, Paula J.; Fischlschweiger, Werner; Coleman, Sylvia E.; Bleiweis, Arnold S.

doi:10.1128/iai.55.11.2695-2700.1987

Cited by 26 publications

(14 citation statements)

References 33 publications

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“…Strain 10557 coaggregated with most A. viscosus and A. naeslundii isolates, whereas S7 did not. A. odontolytieus, which has been reported to coaggregate with few streptococcal strains (3,4), showed no difference in coaggregation with strains S7 and 10557. A. odontolyticus was also found to be ubiquitous and was present in higher numbers than both A. viseosus and A. naeslundii.…”

Section: I'orph Vroiuonas Gingivalis Atcc 33277 Atcc 25260mentioning

confidence: 88%

Dental plaque development on defined streptococcal surfaces

Skopek

Liljemark

Bloomquist

et al. 1993

Oral Microbiology and Immunology

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Coaggregations between bacterial species have been widely studied in vitro but not in the mouth. A new in vivo assay was used to measure the rate and composition of indigenous plaque formation onto bovine enamel chips covered with a continuous layer of bacteria. Chips were covered with Streptococcus oralis ATCC 10557, which coaggregated with many oral species, or Streptococcus gordonii S7, which did not coaggregate with these oral species, and placed in the mouth for 4 and 24 h. There were no differences in the number of most indigenous bacterial species isolated from the two streptococcal surfaces. However, the number of Actinomyces viscosus as a proportion of total Actinomyces spp. was significantly different on the two surfaces at 24 h. With the exception of Actinomyces naeslundii and A. viscosus removed from the S7 surface, all indigenous species increased significantly in number from 4 to 24 h, irrespective of the streptococcal surface. This study demonstrated that interbacterial coaggregation had only a limited effect on in vivo plaque development. Thus suggesting that environmental factors, growth or other adherence phenomena are dominant in in vivo plaque formation.

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Section: I'orph Vroiuonas Gingivalis Atcc 33277 Atcc 25260mentioning

confidence: 88%

Dental plaque development on defined streptococcal surfaces

Skopek

Liljemark

Bloomquist

et al. 1993

Oral Microbiology and Immunology

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“…Additional work is required to detemiine the molecular composition of the fimbrial tufts and how LTA tnay be exposed in these tufts in orientations that will pennit binding to both E tnwteatutn FDC 364 and B. tnatruchotii. In other intergeneric coaggregations involving Streptococcus cricetus and Actinomyces naeslundii or Actinomycis odoiitolyticus it has been suggested that LTA may play a secondary, noti-specific role by strengthening the cell-cell binding (4). Corncob fonnation between E nucleatum ATCC 10953 and S. .sanguis CC5A is also mediated by the polar fimbriae; however, the streptococcal ligand for the sttain ATCC 10953 corncob receptor is unknown.…”

Section: Discussionmentioning

confidence: 99%

Evidence for the existence of two classes of corncob (coaggregation) receptor in Fusobacterium nucleatum

Kaufman

DiRienzo

1988

Oral Microbiology and Immunology

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Oral isolates of Fusobacterium nucleatum have the ability to form distinct coaggregation units, termed corncobs, when mixed with Streptococcus sanguis. Saturation binding kinetics determined for 12 strains of F. nucleatum revealed that these strains could be divided into two groups. One group, represented by F. nucleatum ATCC 10953 reproducibly bound twice as many streptococci as the other group, exemplified by F. nucleatum FDC 364. Other Fusobacterium species tested, including F. mortiferum, Fusobacterium periodonticum, Fusobacterium simiae and Fusobacterium necrophporum, failed to form corncobs with S. sanguis. In inhibition experiments lipoteichoic acid (LTA)‐enriched cell extracts from S. sanguis blocked corncob formation between this bacterium and strain FDC 364 with material containing as little as 50 ng phosphate exhibiting greater than 50% inhibition. Alternatively, 40 times as much material failed to inhibit corncob formation between S. sanguis and F. nucleatum ATCC 10953 or Bacterionema matruchotti. Deacylation of the LTA destroyed its ability to inhibit the fusobacterial coaggregation but produced an active inhibitor in the case of B. matruchotii corncobs. Concerning the fusobacterial coaggregation partner, membrane fractions obtained from strain ATCC 10953 blocked corncob formation while the inhibitory activity of strain FDC 364 was confined to the soluble protein fraction. Corncob‐deficient mutants of F. nucleatum FDC 364 were produced by treating the cells with acridine orange. However, this agent had no effect on the corncob‐forming ability of strain ATCC 10953. The results of these experiments provide convincing evidence that at least 2 independent strain‐specific receptors on F. nucleatum are involved in corncob formation between this bacterium and S. sanguis.

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“…Salivary components have also been reported to promote intergeneric interactions between bacteria (37), and * Corresponding author. cell wall proteins have been reported to promote adherence not only to tooth surfaces but to other plaque microorganisms as well (10,29,36). Therefore, the fate of any given bacterium would depend on many conditions affecting the oral microenvironment.…”

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confidence: 99%

Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1

et al. 1992

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The ability to adhere to salivary agglutinin-coated hydroxyapatite beads and to aggregate in the presence of fluid-phase salivary agglutinin was tested by using 25 isolates of mutans streptococci representing eight serotypes. Both adherence and aggregation activity correlated with expression of the Mr-185,000 cell surface antigen P1 on Streptococcus mutans serotype c, e, and f strains. In addition, it was shown that the P1 molecule itself served as the adhesin of S. mutans serotype c, since adherence was significantly inhibited by the presence of recombinant-specified Mr-150,000 P1. The ability of S. sobrinus strains to adhere or aggregate did not correlate with expression of the P1 cross-reactive antigen SpaA. There was also evidence for interaction with salivary agglutinin, as manifested by aggregation but not adherence of S. rattus serotype b, which does not express a P1 cross-reactive antigen. To understand the interaction of Pl with salivary agglutinin at the molecular level, a panel of 11 anti-Pt monoclonal antibodies was tested for inhibitory activity in adherence and aggregation inhibition assays. Overlapping, but not identical, subsets of monoclonal antibodies were found to inhibit adherence and aggregation, indicating that the interactions of P1 with salivary agglutinin which mediate these two phenomena are different. The localization of functional domains of P1 which may mediate the aggregation and adherence reactions is discussed.

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Intergeneric bacterial coaggregations involving mutans streptococci and oral actinomyces

Cited by 26 publications

References 33 publications

Dental plaque development on defined streptococcal surfaces

Dental plaque development on defined streptococcal surfaces

Evidence for the existence of two classes of corncob (coaggregation) receptor in Fusobacterium nucleatum

Differentiation of salivary agglutinin-mediated adherence and aggregation of mutans streptococci by use of monoclonal antibodies against the major surface adhesin P1

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