Intergeneric complementation by Agrobacterium tumefaciens chromosomal genes and its potential use for linkage mapping

Mapping of Agrobacterium tumefaciens chromosomal genes affecting cellulose synthesis and bacterial attachment to host cells

“…They both mapped to the same region as the cel mutations. This region is distant from the suggested locations for the chvAB genes, which also affect bacterial attachment to host cells (11,13). Since many of the cel and att mutations mapped are apparently located very close to each other, the exact order of the mutations in this region as presented here must be regarded as tentative.…”

“…The TnS insertions in the chvAB mutants map in a few small linked EcoRI fragments of the chromosome (3). These insertions have been mapped by Miller et al (11) and by Waelkens et al (13) to the vicinity of trp-13 on the map of Hooykaas et al (5). The att mutations isolated by Matthysse are all located in one 12-kilobase chromosomal EcoRI fragment (8).…”

“…The mapping procedure used was a modification of that of Waelkens et al (13) and of Hooykaas et al (5). The modifications were made to allow the mapping of the insertion site of TnS.…”