Intergeneric hybrids of Phalaenopsis 2166 x Vanda ‘Saint Valentine’ showing maternal inheritance: Genetic analysis based on ndhE partial gene

Dwiati, Murni; Susanto, Agus Hery; Prayoga, Lucky

doi:10.13057/biodiv/d211119

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Six sults suggested that interspecific hybridization can contribute to increase the genetic variation of Dendrobium section Spatulata. Another study reported that hybridization between two different species produced high variation in all progenies of orchid (Dwiati et al 2020).…”

Section: Flower Morphology Of Parental and Progeny Lines Of Antelope ...mentioning

confidence: 99%

Genetic Variation and Genomic Constitution in The Spatulata Orchid Hybrids (Dendrobium spp.) Derived from Interspecific Hybridization Using SRAP marker

Purwantoro

Setiawan

Nugraha

et al. 2023

Indones. J. Biotechnol.

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Dendrobium is the second largest genus in the Orchidaceae family. Dendrobium section Spatulata is widely cultivated in Indonesia due to its ease of cultivation, high economic value and adaptability, and extended flower shelf life. In this study, we have conducted a breeding program for developing new cultivar of Dendrobium section Spatulata through interspecific hybridization. This study is aimed to investigate the genetic variation and genomic constitution of the eight hybrids and their corresponding parental lines resulted from interspecific hybridization using SRAP markers. Dendrobium section Spatulata hybrids produced by interspecific hybridization are genuine hybrids with substantial genetic variability based on flower morphology, including labellum shapes and color intensities, as well as curly horn shapes and color intensities. The SRAP marker, which was utilized to genotype the hybrid and parental lines, exhibited a significant degree of polymorphism, and may be used to distinguish each accession. The UPGMA dendrogram and PCoA biplot showed that all the hybrids were grouped with their corresponding parental lines based on their genetic background and genomic constitution. These findings are critical for genetic improvement of the Spatulata orchid to develop novel varieties.

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Section: Flower Morphology Of Parental and Progeny Lines Of Antelope ...mentioning

confidence: 99%

Genetic Variation and Genomic Constitution in The Spatulata Orchid Hybrids (Dendrobium spp.) Derived from Interspecific Hybridization Using SRAP marker

Purwantoro

Setiawan

Nugraha

et al. 2023

Indones. J. Biotechnol.

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“…The other three hybrids, i.e., F1.9, F1.11, and F1.14, showed slightly different ndhE sequences from that of Phalaenopsis 2166, and they have also been registered in the NCBI database as MH646651. One of the three hybrids, i.e., F1.14, has a partially spotted reddish-purple leaf that is predictable to produce conspicuously attractive flowers, thus potentially to be developed into a large number of plant individuals (Dwiati et al 2020b;Dwiati and Susanto 2021).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda ‘Saint Valentine’: Application of NAA and TDZ

Dwiati

Susanto²,

Budisantoso³

2022

Nusantara Biosci

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Abstract. Dwiati M, Susanto AH, Budisantoso I. 2022. Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda' Saint Valentine': Application of NAA and TDZ. Nusantara Bioscience 14: 160-165. Intergeneric hybridization between Phalaenopsis 2166 and Vanda' Saint Valentine' has produced a hybrid seedling with several characters potentially developing into plant individuals with flowers of better performance. Therefore, identical clones of the selected hybrid should be developed into PLBs using an in-vitro culture technique employing somatic embryogenesis supported by the application of plant growth regulators. This study aims to unveil the effect of NAA and TDZ in stimulating the formation of identical clones of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda' Saint Valentine'. The experiment was arranged in a factorial Randomized Complete Block Design (RCBD) involving two factors, i.e., types of plant growth regulators and the levels of concentrations of each substance. It was found that the combination of NAA and TDZ significantly affected the growth of the identical clones. Furthermore, the combination of NAA 0.5 mgL-1 and TDZ 1.5 mgL-1 resulted in clones that potentially differentiate into PLBs. This finding indicates that NAA and TDZ should be applied appropriately to stimulate somatic embryogenesis in the intergeneric hybrid.

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Intergeneric hybrids of Phalaenopsis 2166 x Vanda ‘Saint Valentine’ showing maternal inheritance: Genetic analysis based on ndhE partial gene

Cited by 2 publications

References 22 publications

Genetic Variation and Genomic Constitution in The Spatulata Orchid Hybrids (Dendrobium spp.) Derived from Interspecific Hybridization Using SRAP marker

Genetic Variation and Genomic Constitution in The Spatulata Orchid Hybrids (Dendrobium spp.) Derived from Interspecific Hybridization Using SRAP marker

Somatic embryogenesis of the selected intergeneric hybrid between Phalaenopsis 2166 and Vanda ‘Saint Valentine’: Application of NAA and TDZ

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