Intergenic transformation of AtMYB44 confers drought stress tolerance in rice seedlings

Joo, Joungsu; Oh, Nam-Iee; Nguyen, Nguyen Hoai; Lee, Youn Hab; Kim, Yeon-Ki; Song, Sang Ik; Cheong, Jong-Joo

doi:10.1007/s13765-017-0297-5

Cited by 11 publications

(6 citation statements)

References 31 publications

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“…Plant cells produce osmoprotectants to compensate for the water loss caused by salt, drought, or cold stress 11 . High salinity and drought may cause a marked imbalance in redox homeostasis, which drives plant cells to reorganize primary as well as secondary metabolism 12 , 13 . In this process, plant hormones serve as crucial integrators that modulate complex developmental and stress signalling pathways.…”

Section: Introductionmentioning

confidence: 99%

Dual role of SND1 facilitates efficient communication between abiotic stress signalling and normal growth in Arabidopsis

Jeong

Lee

Truong

et al. 2018

Sci Rep

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Certain plant cells synthesize secondary cell walls besides primary cell walls. This biosynthesis is strictly controlled by an array of transcription factors. Here, we show that SND1, a regulator of cell-wall biosynthesis, regulates abscisic acid (ABA) biosynthesis to ensure optimal plant growth. In Arabidopsis, the lack of SND1 and its homolog NST1 leads to the deficiency of secondary cell walls, preventing snd1nst1 double mutant seedlings from growing upright. Compared to wild type seedlings, the snd1 knockout mutant seedlings accumulated less anthocyanin and exhibited low tolerance to salt stress. Compared to wild type seedlings, the snd1 knockout seedlings were more sensitive to salt stress. Although SND1 can bind to the promoter of Myb46, we observed that SND1 binds directly to the promoter of the ABI4 gene, thereby reducing ABA levels under normal growth conditions. Thus, plants adjust secondary cell wall thickening and growth via SND1. SND1 has a dual function: it activates the Myb46 pathway, fostering lignin biosynthesis to produce sufficient cell wall components for growth, while maintaining a low ABA concentration, as it inhibits growth. This dual function of SND1 may help plants modulate their growth efficiently.

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Section: Introductionmentioning

confidence: 99%

Dual role of SND1 facilitates efficient communication between abiotic stress signalling and normal growth in Arabidopsis

Jeong

Lee

Truong

et al. 2018

Sci Rep

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“…However, in our study, we found that the plants harboring overexpressed MYB44 showed enhanced drought tolerance, while myb44-1 was less resistant to drought stress compared to Col-0 ( S4C Fig ), which is consistent with the previous report [ 18 ]. Others also found that overexpression of Arabidopsis MYB44 in rice and in soybean led to an enhanced drought tolerance [ 29 , 30 ], further suggesting the positive role of MYB44 in drought stress. MYB44 was previously proposed to activate transcription [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, the water loss rate is reduced in the plants that MYB44 are overexpressed, as a result, the plants are more drought tolerant [ 18 ]. When Arabidopsis MYB44 is heterologously expressed in soybean and rice, the drought tolerance is significantly improved [ 29 , 30 ], strongly indicating that MYB44 is a positive regulator in drought stress. Yet, the molecular mechanism that how MYB44 functions in drought stress is ambiguous.…”

Section: Introductionmentioning

confidence: 99%

MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis

et al. 2022

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Histone acetylation has been shown to involve in stress responses. However, the detailed molecular mechanisms that how histone deacetylases and transcription factors function in drought stress response remain to be understood. In this research, we show that ENAP1 and ENAP2 are positive regulators of drought tolerance in plants, and the enap1enap2 double mutant is more sensitive to drought stress. Both ENAP1 and ENAP2 interact with MYB44, a transcription factor that interacts with histone deacetylase HDT4. Genetics data show that myb44 null mutation enhances the sensitivity of enap1enap2 to drought stress. Whereas, HDT4 negatively regulates plant drought response, the hdt4 mutant represses enap1enap2myb44 drought sensitive phenotype. In the normal condition, ENAP1/2 and MYB44 counteract the HDT4 function for the regulation of H3K27ac. Upon drought stress, the accumulation of MYB44 and reduction of HDT4 leads to the enrichment of H3K27ac and the activation of target gene expression. Overall, this research provides a novel molecular mechanism by which ENAP1, ENAP2 and MYB44 form a complex to restrict the function of HDT4 in the normal condition; under drought condition, accumulated MYB44 and reduced HDT4 lead to the elevation of H3K27ac and the expression of drought responsive genes, as a result, plants are drought tolerant.

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“…Under drought conditions, the transgenic Arabidopsis exhibited reduced rates of water loss and enhanced tolerance [ 94 ]. Furthermore, transgenic soybean [ 97 ] and rice seedlings [ 98 ] overexpressing AtMYB44 exhibited significantly enhanced drought and salt stress tolerance. It appears that the enhanced osmotic stress tolerance of the transgenic plants was conferred by reduced expression of genes encoding PP2Cs that function as negative regulators of ABA-mediated stomatal closure.…”

Section: Transcriptional Regulation Of Pp2c Gene Expressionmentioning

confidence: 99%

Transcriptional Regulation of Protein Phosphatase 2C Genes to Modulate Abscisic Acid Signaling

Jung

Nguyen

Cheong

2020

IJMS

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The plant hormone abscisic acid (ABA) triggers cellular tolerance responses to osmotic stress caused by drought and salinity. ABA controls the turgor pressure of guard cells in the plant epidermis, leading to stomatal closure to minimize water loss. However, stomatal apertures open to uptake CO2 for photosynthesis even under stress conditions. ABA modulates its signaling pathway via negative feedback regulation to maintain plant homeostasis. In the nuclei of guard cells, the clade A type 2C protein phosphatases (PP2Cs) counteract SnRK2 kinases by physical interaction, and thereby inhibit activation of the transcription factors that mediate ABA-responsive gene expression. Under osmotic stress conditions, PP2Cs bind to soluble ABA receptors to capture ABA and release active SnRK2s. Thus, PP2Cs function as a switch at the center of the ABA signaling network. ABA induces the expression of genes encoding repressors or activators of PP2C gene transcription. These regulators mediate the conversion of PP2C chromatins from a repressive to an active state for gene transcription. The stress-induced chromatin remodeling states of ABA-responsive genes could be memorized and transmitted to plant progeny; i.e., transgenerational epigenetic inheritance. This review focuses on the mechanism by which PP2C gene transcription modulates ABA signaling.

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Intergenic transformation of AtMYB44 confers drought stress tolerance in rice seedlings

Cited by 11 publications

References 31 publications

Dual role of SND1 facilitates efficient communication between abiotic stress signalling and normal growth in Arabidopsis

Dual role of SND1 facilitates efficient communication between abiotic stress signalling and normal growth in Arabidopsis

MYB44-ENAP1/2 restricts HDT4 to regulate drought tolerance in Arabidopsis

Transcriptional Regulation of Protein Phosphatase 2C Genes to Modulate Abscisic Acid Signaling

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