Interglobular Dentin in the Deciduous Tooth

Abstract

Interglobular dentin formation, as described in textbooks of dental histology,' 2 is the result of normal matrix formation but failure of complete fusion of the calcospherites during the calcification period that follows.Schour and Poncher,3 Kronfeld and Schour,4 and Blackerby5 have observed that in deciduous teeth the prenatal dentin appeared homogeneous without interglobular spaces, whereas postnatal deciduous dentin contained varying intensities of interglobular space formation. These observations and the universal appearance of interglobular dentin in permanent teeth reported by Katayama,6 Nishiyama,7 Erva,8 and Nakajima9 have led to the assumption that, as far as dentin calcification is concerned, the prenatal period provides more optimum nutritional conditions than the postnatal period.It was the purpose of this study to examine serial ground sections from a large number of extracted deciduous central and lateral incisors, canines, and first and second molar teeth and to reveal the distribution of interglobular spaces in these organs. A histologic section and a historadiograph of a typical longitudinal ground section are also described. MATERIALS AND METHODSA large random sample of extracted deciduous teeth from native Japanese patients was examined. Most of these teeth were unidentifiable because of exfoliative root resorption and marked coronal attrition. Only recognizable typical teeth, including a few containing very small cavities, were used in this study. The top line of Table 1 shows that 41 central incisors, 47 lateral incisors, 42 canines, 47 first and 42 second molars, or a total of 219 teeth, were included.Each tooth was imbedded in plastic, and labio-or buccolingual longitudinal serial sections, each about 150 u thick, were cut with a thin sectioning machine.* At least four serial sections were obtained from each incisor and twelve from each molar. These serial ground sections were examined under a microscope using transmitted light, a 9X eyepiece and a 1oX objective.Each type of tooth was classified according to the frequency of occurrence of the interglobular spaces seen in the serial ground sections, and the values arranged in the table in one of three groups as follows: Group I, no interglobular spaces; Group II, a few interglobular spaces; and Group III, many interglobular spaces.

Micro‐CT assessment of dental mineralization defects indicative of vitamin D deficiency in two 17th–19th century Dutch communities

“…If there are adequate nutritional conditions during the processes of formation and calcification, the matrix will appear homogenous and fusion of calcospherites, spheres containing calcium salts, will be complete (D'Ortenzio et al, ; Hillson, ). However, when vitamin D levels are inadequate, some of the calcospherites fail to fuse, which is visible as poorly mineralized patches of dentine with a bubble‐like appearance, referred to as IGD (D'Ortenzio et al, ; Isokawa, Kosakai, & Kajiyama, ; Vital et al, ). The presence of IGD is clinically associated with conditions disrupting mineralization by affecting vitamin D, calcium and/or phosphate levels (Chaussain‐Miller et al, ; McDonnell, Derkson, Zhang, & Hlady, ; Souza, Soares, Alves dos Santos, & Vaisbisch, ; Vital et al, ).…”

Interglobular Spaces in Some Mammalian Teeth

“…Investigators including KATAYAMA [7], NISHIYAMA [8], EVRA [9], NAKAJIMA [10] and ISOKAWA et al [11] all confirmed the existence of interglobular space in human teeth with nearly 100 % occurrence. With other mammalian teeth, however, the existence of this interglobular space has not been confirmed.…”

“…

KOLLMANN[5] to be that portion of hypocalcified area occured by an imperfect union of calcospherites appearing in the calcification of dentin through its formative process.In the published native literature, ISOKAWA et al[6] established the interglobular space as an imperfectly calcified portion as a result of their historadiographic analysis.In an effort to study universal morphology of the interglobular space, KATAYAMA [7], NISHIYAMA [8], ERVA [9] and NAKAJIMA[10] used the permanent teeth, whereas ISOKAWA et al [11] made use of the deciduous teeth, the latter carrying out observations by means of semi-serial ground sections. Consensus of their opinions indicates that the interglobular space can be regarded as a universal structure except for the deciduous incisors and canines in man.

On the other hand, FUJITA [12] in his textbook on Dental Histology describes that the interglobular space is altogether lacking in teeth other than those of man and, if there should be found at all, it is a very rare phenomenon.

…”

“…In an effort to study universal morphology of the interglobular space, KATAYAMA [7], NISHIYAMA [8], ERVA [9] and NAKAJIMA[10] used the permanent teeth, whereas ISOKAWA et al [11] made use of the deciduous teeth, the latter carrying out observations by means of semi-serial ground sections. Consensus of their opinions indicates that the interglobular space can be regarded as a universal structure except for the deciduous incisors and canines in man.…”

Scanning electron microscopic observations of dentinal tubules in interglobular dentin.

“…Morphological studies on the interglobular dentin that exists in human teeth are under way using microradiography [1,2] or scanning electron microscopy [3]. Dept.…”