Interkingdom responses of flies to bacteria mediated by fly physiology and bacterial quorum sensing

Tomberlin, Jeffery K.; Crippen, Tawni L.; Tarone, Aaron M.; Singh, Baneshwar; Adams, Kelsey; Rezenom, Yohannes H.; Benbow, M. Eric; Flores, Micah; Longnecker, Michael T.; Pechal, Jennifer L.; Russell, David H.; Beier, Ross C.; Wood, Thomas K.

doi:10.1016/j.anbehav.2012.09.013

Cited by 79 publications

(36 citation statements)

References 30 publications

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“…Postmortem microbial communities shift when a carcass is decomposing, probably due to rupture, increased resource availability, or the proliferation of insects. It has been shown that the cadaver microbial community can influence insect activity and vice versa [42][43][44][45]. Added to this is likely an example of Bresource selects community^ [46] where the different stages of decomposition to the control soil on each accumulated degree day (ADD).…”

Section: Discussionmentioning

confidence: 99%

Carcass mass has little influence on the structure of gravesoil microbial communities

et al. 2015

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Little is known about how variables, such as carcass mass, affect the succession pattern of microbes in soils during decomposition. To investigate the effects of carcass mass on the soil microbial community, soils associated with swine (Sus scrofa domesticus) carcasses of four different masses were sampled until the 15th day of decomposition during the month of June in a pasture near Lincoln, Nebraska. Soils underneath swine of 1, 20, 40, and 50 kg masses were investigated in triplicate, as well as control sites not associated with a carcass. Soil microbial communities were characterized by sequencing the archaeal, bacterial (16S), and eukaryotic (18S) rRNA genes in soil samples. We conclude that time of decomposition was a significant influence on the microbial community, but carcass mass was not. The gravesoil associated with 1 kg mass carcasses differs most compared to the gravesoil associated with other carcass masses. We also identify the 15 most abundant bacterial and eukaryotic taxa, and discuss changes in their abundance as carcass decomposition progressed. Finally, we show significant decreases in alpha diversity for carcasses of differing mass in pre-carcass rupture (days 0, 1, 2, 4, 5, and 6 postmortem) versus post-carcass rupture (days 9 and 15 postmortem) microbial communities.

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Section: Discussionmentioning

confidence: 99%

Carcass mass has little influence on the structure of gravesoil microbial communities

et al. 2015

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“…Importantly, the variation in colonizing bacteria across carcasses would have to be uniquely attractive to each species for intraspeciÞc aggregation to be greater than interspeciÞc aggregation. There is much evidence that bacteria and associated compounds can serve as semiochemicals for insects (Tomberlin et al 2012;reviewed in Davis et al 2013), and we believe this Þeld of research holds much promise for unraveling the proximate mechanisms governing intraspeciÞc aggregation across patchy resources like carrion.…”

Section: Proximate Mechanisms Of Aggregationmentioning

confidence: 97%

The Role of Spatial Aggregation in Forensic Entomology: Table 1.

et al. 2014

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A central concept in forensic entomology is that arthropod succession on carrion is predictable and can be used to estimate the postmortem interval (PMI) of human remains. However, most studies have reported significant variation in successional patterns, particularly among replicate carcasses, which has complicated estimates of PMIs. Several forensic entomology researchers have proposed that further integration of ecological and evolutionary theory in forensic entomology could help advance the application of succession data for producing PMI estimates. The purpose of this essay is to draw attention to the role of spatial aggregation of arthropods among carrion resources as a potentially important aspect to consider for understanding and predicting the assembly of arthropods on carrion over time. We review ecological literature related to spatial aggregation of arthropods among patchy and ephemeral resources, such as carrion, and when possible integrate these results with published forensic literature. We show that spatial aggregation of arthropods across resources is commonly reported and has been used to provide fundamental insight for understanding regional and local patterns of arthropod diversity and coexistence. Moreover, two suggestions are made for conducting future research. First, because intraspecific aggregation affects species frequency distributions across carcasses, data from replicate carcasses should not be combined, but rather statistically quantified to generate occurrence probabilities. Second, we identify a need for studies that tease apart the degree to which community assembly on carrion is spatially versus temporally structured, which will aid in developing mechanistic hypotheses on the ecological factors shaping community assembly on carcasses.

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“…Microbes can also influence attraction of insects to their hosts (Hilker and Meiners 2002). For instance, Proteus mirabilis attracts L. sericata (Ma et al 2012b;Tomberlin et al 2012), Musca domestica Linnaeus (Diptera: Muscidae) females have been shown to prefer to oviposit on eggs coated with certain Gram-positive bacteria (Lam et al 2007), and Aedes aegypti (Linnaeus) (Diptera: Culicidae) prefer oviposition on a mixture of 14 bacteria isolates from bamboo leaf infusion compared to water as a control (Ponnusamy et al 2008). Since bacteria and their associated metabolites can influence blow fly behavior, it seems likely that bacterial research with these flies will have repercussions for forensic, medical, veterinary, and agricultural applications (Tomberlin et al 2012).…”

Section: Introductionmentioning

confidence: 99%

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

Singh

Crippen

Zheng

et al. 2014

Appl Microbiol Biotechnol

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Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.

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Interkingdom responses of flies to bacteria mediated by fly physiology and bacterial quorum sensing

Cited by 79 publications

References 30 publications

Carcass mass has little influence on the structure of gravesoil microbial communities

Carcass mass has little influence on the structure of gravesoil microbial communities

The Role of Spatial Aggregation in Forensic Entomology: Table 1.

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

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