DARWIN (1854), P-I 97> an d again on p. 242 vividly describes the great variability of Balanus amphitrite and B. tintinnabulum and the difficulty he had in coming to the decision as to whether the various varieties he established for these two species should be regarded as varieties or as separate species. Balanus amphitrite like B.tintinnabulum is frequently found attached to ships and much of the extreme tendency to vary may well, as Darwin himself suggested, be due to their frequently being transported to new localities. Since Darwin's time, many workers have been faced with the same problem and are hampered by the fact that Darwin was unable to publish many illustrations and that his descriptions of the animal parts of the varieties are inadequate by present standards more than a century later. It is now forty-five years since Pilsbry (1916, pp. 93-94) wrote, " The definition of the sub-species of Balanus amphitrite is a very intricate problem . . . first of all the Darwin collection must be restudied and type localities for his varieties selected".The purpose of this paper is to publish the results of such a study. Much of the material studied by Darwin no longer exists ; but there is a cabinet of dry shells mounted on slabs in the British Museum. In the account which follows these specimens are referred to the slab to which they were attached, e.g. Slab 17 . 1 . 22.There are also a number of microscopic slides of mouth-parts and other appendages dissected by Darwin now in the University Museum of Zoology, Cambridge ; these