Interleukin-1βModulates Melatonin Secretion in Ovine Pineal Gland:Ex VivoStudy

Herman, Andrzej Przemysław; Bochenek, Joanna; Skipor, Janina; Król, Karolina; Krawczyńska, Agata; Antushevich, Hanna; Pawlina, Bartosz; Marciniak, Elżbieta; Tomaszewska-Zaremba, Dorota

doi:10.1155/2015/526464

Cited by 15 publications

(18 citation statements)

References 39 publications

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“…In sheep from the SN photoperiod, no influence of IL-1 β treatment on both gene and protein expression of AANAT was found. These results are generally consistent with our previous results obtained during an ex vivo study, which showed that IL-1 β might have reduced the level of AANAT protein [ 15 ]. The modulatory action of IL-1 β on AANAT expression seems to be targeted on the posttranscriptional level of the expression of this enzyme because the same study showed a lack of any effect of IL-1 β treatment on AANAT mRNA expression.…”

Section: Discussionsupporting

confidence: 93%

“…It was clearly demonstrated that the injection of IL-1 β into the IIIv suppressed melatonin release in sheep, regardless of the photoperiod. The data obtained here fully support the results of our ex vivo studies demonstrating that IL-1 β suppressed NE-stimulated melatonin secretion from explants of ovine pineal glands [ 15 ]. The existence of an interplay between Il-1 β and the pineal gland has also been found in experiments on rats indicating a significant dose-dependent decrease in serum melatonin levels under the influence of exogenous recombinant human IL-1 β , which was abolished by an anti-human IL-1 receptor antibody [ 12 ].…”

Section: Discussionsupporting

confidence: 88%

“…A few studies have shown that the secretory activity of pinealocytes can be modified by antigenic stimulation [ 9 ], histamines [ 10 ], cytokines [ 11 , 12 ], and prostaglandins [ 13 , 14 ]. Also our previous ex vivo study showed that the pleiotropic proinflammatory cytokine interleukin- (IL-) 1 β suppressed melatonin secretion in ovine pineal glands [ 15 ]. Immune mediators have easy access to the pineal gland because it is a part of the brain that lacks the blood-brain barrier [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Central Interleukin-1βSuppresses the Nocturnal Secretion of Melatonin

Herman

Bochenek

Król

et al. 2016

Mediators of Inflammation

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In vertebrates, numerous processes occur in a rhythmic manner. The hormonal signal reliably reflecting the environmental light conditions is melatonin. Nocturnal melatonin secretion patterns could be disturbed in pathophysiological states, including inflammation, Alzheimer's disease, and depression. All of these states share common elements in their aetiology, including the overexpression of interleukin- (IL-) 1β in the central nervous system. Therefore, the present study was designed to determine the effect of the central injection of exogenous IL-1β on melatonin release and on the expression of the enzymes of the melatonin biosynthetic pathway in the pineal gland of ewe. It was found that intracerebroventricular injections of IL-1β (50 µg/animal) suppressed (P < 0.05) nocturnal melatonin secretion in sheep regardless of the photoperiod. This may have resulted from decreased (P < 0.05) synthesis of the melatonin intermediate serotonin, which may have resulted, at least partially, from a reduced expression of tryptophan hydroxylase. IL-1β also inhibited (P < 0.05) the expression of the melatonin rhythm enzyme arylalkylamine-N-acetyltransferase and hydroxyindole-O-methyltransferase. However, the ability of IL-1β to affect the expression of these enzymes was dependent upon the photoperiod. Our study may shed new light on the role of central IL-1β in the aetiology of disruptions in melatonin secretion.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Central Interleukin-1βSuppresses the Nocturnal Secretion of Melatonin

Herman

Bochenek

Król

et al. 2016

Mediators of Inflammation

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“…According to the results of the present study, it could be suggested that the physiological status of an animal may influence the responsiveness of the PT to both GnRH and melatonin action. These findings confirm the results of previous studies showing that the glands kept their ‘memory’ of the events triggered by LPS exposure, and this affected tissue explant activity during in vitro culture [ 14 , 29 , 30 , 31 ]. It was found that the PT explants isolated from ewes in an acute inflammatory state were characterized by significantly lower GnRH-dependent release of LH in comparison with the tissues collected from saline-treated individuals.…”

Section: Discussionsupporting

confidence: 91%

Endotoxin-Induced Inflammation Suppresses the Effect of Melatonin on the Release of LH from the Ovine Pars Tuberalis Explants—Ex Vivo Study

2017

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The secretion of the hormone melatonin reliably reflects environmental light conditions. Among numerous actions, in seasonal breeders, melatonin may regulate the secretion of the gonadotropins acting via its corresponding receptors occurring in the Pars Tuberalis (PT). However, it was previously found that the secretory activity of the pituitary may be dependent on the immune status of the animal. Therefore, this study was designed to determine the role of melatonin in the modulation of luteinizing hormone (LH) secretion from the PT explants collected from saline- and endotoxin-treated ewes in the follicular phase of the oestrous cycle. Twelve Blackhead ewes were sacrificed 3 h after injection with lipopolysaccharide (LPS; 400 ng/kg) or saline, and the PTs were collected. Each PT was cut into 4 explants, which were then divided into 4 groups: I, incubated with ‘pure’ medium 199; II, treated with gonadotropin-releasing hormone (GnRH) (100 pg/mL); III, treated with melatonin (10 nmol/mL); and IV, incubated with GnRH and melatonin. Melatonin reduced (p < 0.05) GnRH-induced secretion of LH only in the PT from saline-treated ewes. Explants collected from LPS-treated ewes were characterized by lower (p < 0.05) GnRH-dependent response in LH release. It was also found that inflammation reduced the gene expression of the GnRH receptor and the MT1 melatonin receptors in the PT. Therefore, it was shown that inflammation affects the melatonin action on LH secretion from the PT, which may be one of the mechanisms via which immune/inflammatory challenges disturb reproduction processes in animals.

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“…However, the role of melatonin in the regulation of the course of the inflammatory response may be more ambiguous. On the one hand melatonin might promote early phases of inflammation, but on the other hand contribute to its attenuation in order to avoid complications of chronic inflammation (Herman et al, 2015). It was also found that melatonin administration normalize IL1, IL6 and TNF mRNA levels during cardiac inflammation which supports the thesis about dual role of melatonin in modulation of the inflammation (Mauriz et al, 2013).…”

Section: Discussionmentioning

confidence: 55%