Interleukin-1, interleukin-6 and the germ cell-Sertoli cell cross-talk

Jégou, Bernard; Cudicini, C.; Gomez, Edith; Stephan, J. Reshkin

doi:10.1071/rd9950723

Cited by 30 publications

(25 citation statements)

References 44 publications

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“…Therefore, the synchronization process of the spermatogenesis cycle may depend on a proteolytic message (as summarized in Fig. 3), revisiting the pioneering hypothesis of Regaud and Roosen-Runge (referenced in Jégou et al 1995. Two other cytokines have proven to be essential at least in the passage of the testis barrier by preleptotene spermatocytes.…”

Section: Spermatogenesis and The Apical Migration Of Germ Cells Towarmentioning

confidence: 99%

“…The plasminogen activation system in the testis Interestingly, interleukin 1a production is enhanced upon phagocytosis of residual bodies (Jégou et al 1995), and interleukin 1a is known to stimulate DNA synthesis in spermatogonia and spermatocytes. Thus, residual bodies upon phagocytosis could trigger the induction of Sertoli interleukin 1a, which would lead to the enhancement of the PA activity facilitating germ cell migration in the adluminal compartment and their entry into meiosis, and in the stimulation of germ cell proliferation preceding the initiation of a new wave of spermatogenesis.…”

Section: Spermatogenesis and The Apical Migration Of Germ Cells Towarmentioning

confidence: 99%

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Serine proteases and serine protease inhibitors in testicular physiology: the plasminogen activation system

Magueresse-Battistoni

2007

Reproduction

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The testis is an organ in which a series of radical remodeling events occurs during development and in adult life. These events likely rely on a sophisticated network of proteases and complementary inhibitors, including the plasminogen activation system. This review summarizes our current knowledge on the testicular occurrence and expression pattern of members of the plasminogen activation system. The various predicted functions for these molecules in the establishment and maintenance of the testicular architecture and in the process of spermatogenesis are presented.

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Section: Spermatogenesis and The Apical Migration Of Germ Cells Towarmentioning

confidence: 99%

Section: Spermatogenesis and The Apical Migration Of Germ Cells Towarmentioning

confidence: 99%

Serine proteases and serine protease inhibitors in testicular physiology: the plasminogen activation system

Magueresse-Battistoni

2007

Reproduction

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“…In this case, SC are prime candidates. These cells interact directly with developing germ cells (10,11) and express a functional AR with highest expression occurring at stages of the spermatogenic cycle when androgens are thought to act (1). Major changes in overall protein synthesis have been shown to be androgen-dependent at these stages (1,12), but despite extensive investigations, by using either in vivo or in vitro approaches, limited changes in expression of individual proteins by SC in response to androgens have been shown (1,12).…”

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confidence: 99%

A Sertoli cell-selective knockout of the androgen receptor causes spermatogenic arrest in meiosis

Gendt

Swinnen

Saunders

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

715

646

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Androgens control spermatogenesis, but germ cells themselves do not express a functional androgen receptor (AR). Androgen regulation is thought to be mediated by Sertoli and peritubular myoid cells, but their relative roles and the mechanisms involved remain largely unknown. Using Cre͞loxP technology, we have generated mice with a ubiquitous knockout of the AR as well as mice with a selective AR knockout in Sertoli cells (SC) only. Mice with a floxed exon 2 of the AR gene were crossed with mice expressing Cre recombinase ubiquitously or selectively in SC (under control of the anti-Mü llerian hormone gene promoter). AR knockout males displayed a complete androgen insensitivity phenotype. Testes were located abdominally, and germ cell development was severely disrupted. In contrast, SC AR knockout males showed normal testis descent and development of the male urogenital tract. Expression of the homeobox gene Pem, which is androgen-regulated in SC, was severely decreased. Testis weight was reduced to 28% of that in WT littermates. Stereological analysis indicated that the number of SC was unchanged, whereas numbers of spermatocytes, round spermatids, and elongated spermatids were reduced to 64%, 3%, and 0% respectively of WT. These changes were associated with increased germ cell apoptosis and grossly reduced expression of genes specific for late spermatocyte or spermatid development. It is concluded that cell-autonomous action of the AR in SC is an absolute requirement for androgen maintenance of complete spermatogenesis, and that spermatocyte͞spermatid development͞ survival critically depends on androgens.

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Section: Discussionmentioning

confidence: 99%

“…Mature Sertoli cells are capable of producing and secreting IL-1␣ [33]. It is known that during the process of sexual maturation, Sertoli cells isolated from rat testes increase the amount of secreted IL-1␣ [33]. While Sertoli cells isolated from 45-day-old rats secrete twice as much as similar cultures prepared from 35-day-old animals, there is no detectable amount of IL-1␣ present in Sertoli cell-conditioned media from prepubertal animals (20 day old) [33].…”

Section: Discussionmentioning

confidence: 99%

Induction of Interleukin-1α Production in Murine Sertoli Cells by Interleukin-11

Zeyse¹,

Lunenfeld²,

BECK³

et al. 2000

Biology of Reproduction

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In the present study we examined the involvement of interleukin (IL)-1alpha, -1beta, FSH, and lipopolysaccharide (LPS) in the regulation of IL-1alpha and -1beta production by Sertoli cells under in vitro conditions. Sertoli cell cultures from immature mice produced constitutively basal levels of intracellular IL-1alpha. Stimulation of Sertoli cell cultures with LPS (5 microgram/ml) resulted in a maximal production of intracellular IL-1alpha 2 h after the stimulation. Thereafter, these levels decreased but remained significantly higher within 24 h after stimulation than those in control cultures. The effect of LPS on IL-1alpha production was dose dependent. FSH did not show any effect on intracellular IL-1alpha production by Sertoli cells. IL-1alpha could not be detected in supernatants of unstimulated or stimulated Sertoli cell cultures. Sertoli cell cultures stimulated with recombinant IL-1alpha induced optimal intracellular levels of IL-1alpha within 2 h of stimulation. These levels remained high 24 h after stimulation. However, stimulation of Sertoli cell cultures with IL-1beta induced a peak of IL-1alpha production 8 h after stimulation. These levels decreased 24 h after the stimulation but were still found to be significantly higher than those in control cultures. The addition of IL-1 receptor antagonist (IL-1ra) to Sertoli cell cultures did not significantly alter their capacity to produce IL-1alpha. However, the stimulatory effects of recombinant IL-1alpha on IL-1alpha production by Sertoli cell cultures were reversed by the concomitant addition of recombinant IL-1ra. No immunoreactive IL-1beta could be detected in lysates or conditioned media of immature murine Sertoli cells under any of the stimulatory conditions outlined. Our results may suggest the involvement of physiological (IL-1) and pathophysiological factors (LPS) in the regulation of spermatogenesis and spermiogenesis processes and male fertility.

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Interleukin-1, interleukin-6 and the germ cell-Sertoli cell cross-talk

Cited by 30 publications

References 44 publications

Serine proteases and serine protease inhibitors in testicular physiology: the plasminogen activation system

Serine proteases and serine protease inhibitors in testicular physiology: the plasminogen activation system

A Sertoli cell-selective knockout of the androgen receptor causes spermatogenic arrest in meiosis

Induction of Interleukin-1α Production in Murine Sertoli Cells by Interleukin-11

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