Interleukin-1 is cytoprotective, antisecretory, stimulates PGE2 synthesis by the stomach, and retards gastric emptying

Robert, André; Olafsson, Adalsteinn S.; Lancaster, C. Roy D.; Zhang, Weirong

doi:10.1016/0024-3205(91)90405-z

Cited by 173 publications

(82 citation statements)

References 26 publications

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“…In hu-man gastric fibroblasts, stimulation with IL-1␤ induces COX-2-dependent PGE 2 generation (27), leading to the expression of hepatocyte growth factor (27,28), which stimulates proliferation of rabbit gastric epithelial cells (28,29). Furthermore, cytoprotection by IL-1␤ against ethanol-induced gastric injury was shown to be dependent on the generation of PGE 2 in an animal model (30). Consequently, it is conceivable that TGF-␣ and IL-1␤ are implicated in the healing of gastric lesions through, at least in part, COX-2-dependent PGE 2 generation.…”

mentioning

confidence: 97%

Secretory Phospholipase A2 Mediates Cooperative Prostaglandin Generation by Growth Factor and Cytokine Independently of Preceding Cytosolic Phospholipase A2 Expression in Rat Gastric Epithelial Cells

Akiba

Hatazawa

Ono

et al. 2001

Journal of Biological Chemistry

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mentioning

confidence: 97%

Secretory Phospholipase A2 Mediates Cooperative Prostaglandin Generation by Growth Factor and Cytokine Independently of Preceding Cytosolic Phospholipase A2 Expression in Rat Gastric Epithelial Cells

Akiba

Hatazawa

Ono

et al. 2001

Journal of Biological Chemistry

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“…PGE 2 is synthesised in gastric glands and its synthesis is enhanced by many factors such as cytokines (Robert et al 1991) or cGMP via NO (Uno et al 1997). PGE 2 has been proposed as a key mediator of mucosal defence.…”

Section: Figure 10mentioning

confidence: 99%

“…On the other hand, prostaglandin E 2 (PGE 2 ) is generally known to be a local chemical mediator that protects the gastric mucosa from acid-peptic injury. PGE 2 synthesis is reported to be stimulated in gastric mucous cells in the presence of biologically active substances, such as cytokines, nitric oxide (NO) and ACh (Robert et al 1991;Uno et al 1997). McQueen et al (1983) reported that prostaglandins could increase the thickness of the mucosal gel layer.…”

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confidence: 99%

EP1 and EP4 Receptors Mediate Exocytosis Evoked by Prostaglandin E₂ in Guinea‐Pig Antral Mucous Cells

Ohnishi

Shimamoto

Katsu

et al. 2001

Experimental Physiology

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Effects of prostaglandin E2 (PGE2) on exocytosis of mucin were studied in mucous cells isolated from guinea‐pig antrum using video‐microscopy. Stimulation with PGE2 elicited a sustained increase in the frequency of exocytotic events in a dose‐dependent manner, which was under regulation by both Ca2+ and cAMP. Stimulation with a selective prostanoid EP4 receptor agonist (ONO‐AEI‐329, 10 μM), which activates cAMP signals, elicited a sustained increase in the frequency of exocytotic events (30% of that evoked by 1 μM PGE2). Stimulation with an EP1 agonist (17‐P‐T‐PGE2, 1 μM), which activates Ca2+ signals, increased the frequency of exocytotic events to a lesser extent (5% of that evoked by 1 μM PGE2), while addition of an EP1 antagonist (ONO‐8713, 10 μM) decreased the frequency of exocytotic events (approximately 40% of that evoked by 1 μM PGE2). However, addition of the EP1 agonist potentiated the frequency of exocytotic events evoked by the EP4 agonist or forskolin (which elevates cAMP levels) and increased the sensitivity of the exocytotic events to forskolin. These results suggest that the Ca2+ signal activated via the EP1 receptor potentiates the cAMP‐regulated exocytotic events activated via the EP4 receptor during PGE2 stimulation, by increasing the sensitivity of the exocytotic response to cAMP. In conclusion, exocytotic events in PGE2‐stimulated antral mucous cells were regulated by interactions between EP1 and EP4 receptors.

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“…To facilitate evaluation of enzyme compositions, formulations, and dosing regimens, we sought to develop a rodent model for gluten digestion. A number of studies (Robert et al, 1991;Matsuda et al, 1993;Burton-Freeman et al, 1997;Izbeki et al, 2001;Overhaus et al, 2004;Turan and Ozdemir, 2004) have exploited the intrinsic similarities between gastrointestinal metabolism of food in rats and hu-mans. Rats may be an effective model for gluten digestion because they are able to eat and digest a significant level of food (on the order of several grams, which would represent a physiological gluten load for a Celiac patient) in a suitable time frame (i.e., in approximately 5-8 h) (Kaneko et al, 1995).…”

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confidence: 99%

Effect of Barley Endoprotease EP-B2 on Gluten Digestion in the Intact Rat

Gass¹,

Vora²,

Bethune³

et al. 2006

J Pharmacol Exp Ther

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Celiac Sprue is a multifactorial disease characterized by an intestinal inflammatory response to ingested gluten. Proteolytically resistant gluten peptides from wheat, rye, and barley persist in the intestinal lumen and elicit an immune response in genetically susceptible individuals. Here, we demonstrate the in vivo ability of a gluten-digesting protease ("glutenase") to accelerate the breakdown of a gluten-rich solid meal. The proenzyme form of endoprotease B, isoform 2 from Hordeum vulgare (EP-B2), was orally administered to adult rats with a solid meal containing 1 g of gluten. Gluten digestion in the stomach and small intestine was monitored as a function of enzyme dose and time by high-performance liquid chromatography and mass spectrometry. In the absence of supplementary EP-B2, gluten was solubilized and proteolyzed to a limited extent in the stomach and was hydrolyzed and assimilated mostly in the small intestine. In contrast, EP-B2 was remarkably effective at digesting gluten in the rat stomach in a dose-and time-dependent fashion. At a 1:25 EP-B2/gluten dose, the gastric concentration of the highly immunogenic 33-mer gliadin peptide was reduced by more than 50-fold within 90 min with no overt signs of toxicity. Evaluation of EP-B2 as an adjunct to diet control is therefore warranted in celiac patients.

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Interleukin-1 is cytoprotective, antisecretory, stimulates PGE2 synthesis by the stomach, and retards gastric emptying

Cited by 173 publications

References 26 publications

Secretory Phospholipase A2 Mediates Cooperative Prostaglandin Generation by Growth Factor and Cytokine Independently of Preceding Cytosolic Phospholipase A2 Expression in Rat Gastric Epithelial Cells

Secretory Phospholipase A2 Mediates Cooperative Prostaglandin Generation by Growth Factor and Cytokine Independently of Preceding Cytosolic Phospholipase A2 Expression in Rat Gastric Epithelial Cells

EP1 and EP4 Receptors Mediate Exocytosis Evoked by Prostaglandin E₂ in Guinea‐Pig Antral Mucous Cells

Effect of Barley Endoprotease EP-B2 on Gluten Digestion in the Intact Rat

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Interleukin-1 is cytoprotective, antisecretory, stimulates PGE2 synthesis by the stomach, and retards gastric emptying

Cited by 173 publications

References 26 publications

Secretory Phospholipase A2 Mediates Cooperative Prostaglandin Generation by Growth Factor and Cytokine Independently of Preceding Cytosolic Phospholipase A2 Expression in Rat Gastric Epithelial Cells

Secretory Phospholipase A2 Mediates Cooperative Prostaglandin Generation by Growth Factor and Cytokine Independently of Preceding Cytosolic Phospholipase A2 Expression in Rat Gastric Epithelial Cells

EP1 and EP4 Receptors Mediate Exocytosis Evoked by Prostaglandin E2 in Guinea‐Pig Antral Mucous Cells

Effect of Barley Endoprotease EP-B2 on Gluten Digestion in the Intact Rat

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EP1 and EP4 Receptors Mediate Exocytosis Evoked by Prostaglandin E₂ in Guinea‐Pig Antral Mucous Cells