Interleukin-1-mediated PGE2 production and sphingomyelin metabolism. Evidence for the regulation of cyclooxygenase gene expression by sphingosine and ceramide.

Ballou, Leslie R.; Chao, Chun‐Cheih; Holness, M. A.; Barker, S.; Raghow, Rajendra

doi:10.1016/s0021-9258(19)88663-5

Cited by 258 publications

(13 citation statements)

References 36 publications

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“…The hydrolysis of SM occurs rapidly upon exposure of the cells to exogenous SMase or to agonists that activate endogenous SMases. Such agonists include TNF-ot, IL-113, IFN-% let,25-dihydroxyvitamin D3, and nerve growth factor (12)(13)(14)(33)(34)(35)(36).…”

Section: Discussionmentioning

confidence: 99%

Role of the ceramide-signaling pathway in cytokine responses to P-fimbriated Escherichia coli.

Hedlund

Svensson

Nilsson

et al. 1996

The Journal of Experimental Medicine

154

114

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SummaryEscherichia coli express fimbriae-associated adhesins through which they attach to mucosal cells and activate a cytokine response. The receptors for E. coli P fimbriae are the globoseries of glycosphingolipids; Galc~l--)4Gall3-containing oligosaccharides bound to ceramide in the outer leaflet of the lipid bilayer. The receptors for type 1 fimbriae are mannosylated glycoproteins rather than glycolipids. This study tested the hypothesis that P-fimbriated E. coli elicit a cytokine response through the release of ceramide in the receptor-bearing cell. We used the A498 human kidney cell line, which expressed functional receptors for P and type 1 fimbriae and secreted higher levels of interleukin (IL)-6 when exposed to the fimbriated strains than to isogenic nonfimbriated controls. P-fimbriated E. coli caused the release of ceramide and increased the phosphorylation of ceramide to ceramide 1-phosphate. The IL-6 response to P-fimbriated E. coli was reduced by inhibitors ofserine/threonine kinases but not by other protein kinase inhibitors. In contrast, ceramide levels were not influenced by type 1-fimbriated E. coli, and the IL-6 response was insensitive to the serine/threonine kinase inhibitors. These results demonstrate that the ceramide-signaling pathway is activated by P-fimbriated E. coli, and that the receptor specificity of the P fimbriae influences this process. We propose that this activation pathway contributes to the cytokine induction by P-fimbriated E. coli in epithelial cells.

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Section: Discussionmentioning

confidence: 99%

Role of the ceramide-signaling pathway in cytokine responses to P-fimbriated Escherichia coli.

Hedlund

Svensson

Nilsson

et al. 1996

The Journal of Experimental Medicine

154

114

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“…Additional research is needed to examine this relationship as well as to examine the biological activities of these ceramides as potential mediators of periodontal destruction. Finally, the recently discovered properties of ceramides to act as biological signaling agents (20)(21)(22)(23)(24) may be relevant to the presence of bacterial ceramides on teeth root surfaces and in diseased gingival tissues.…”

Section: Discussionmentioning

confidence: 99%

Novel ceramides recovered from Porphyromonas gingivalis: relationship to adult periodontitis

Nichols

1998

Journal of Lipid Research

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The primary purpose of this study was to characterize the major structural features of ceramides recovered from Porphyromonas gingivalis , a suspected periodontal pathogen. Complex lipids extracted from P. gingivalis were treated with N,O-bis(trimethylsilyl)-trifluoroacetamide and analyzed using gas chromatography-mass spectrometry. Mass spectra of lipid derivatives revealed cleavage products consistent with structures of four major ceramides. Two of the major ceramides are proposed to contain long chain bases of either 2-amino-1,3-octadecanediol or 2-amino-1,3-nonadecanediol in amide linkage to 3-hydroxy iso branched C 17:0 . The remaining major ceramides are proposed to contain either 2-amino-1,3-octadecanediol or 2-amino-1,3-nonadecanediol in amide linkage to C 17:1 . Alkaline hydrolysis of P. gingivalis lipids and subsequent formation of suitable derivatives revealed 3-hydroxy iso branched C 17:0 , C 17:1 , 2-amino-1,3-octadecanediol, and 2-amino-1,3-nonadecanediol as hydrolysis products. Therefore, the constitutive fatty acids and long chain bases recovered in alkaline hydrolysis products of P. gingivalis lipids are consistent with the proposed ceramide structures. The next goal of this study was to investigate whether these bacterial ceramides exist in lipid extracts of human teeth and gingival tissue at sites of severe adult periodontitis. Using selected ion monitoring of characteristic ions and retention times for each ceramide described above, lipids from teeth and gingival tissue were shown to contain primarily the ceramides containing C 17:1 . It is concluded that P. gingivalis synthesizes at least four major ceramides and two of these ceramides are selectively adsorbed to diseased tooth surfaces and may penetrate into diseased gingival tissue.-Nichols, F. C. Novel ceramides recovered from Porphyromonas gingivalis : relationship to adult periodontitis.

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“…More recent studies have demonstrated that following the addition of these compounds, hours [76], and a gradual increase occurring after several hours and persisting for 24 hours or longer [106]. Surpris-both short-and long-chain sphingomyelins and glycosphingolipids are synthesized as well as free sphingosine, ingly, these patterns can be observed with the same agonist (IL-1␤) [76,[104][105][106][107] and are not associated with long-chain ceramides, and long-chain sphingomyelins.…”

Section: Regulation Of Metabolism Mammalian Glycosphingolipids the Reaction Occurs Onmentioning

confidence: 99%

Sphingolipids

Shayman

2000

Kidney International

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A significant corpus of work over the last decade has firmly established an important role for sphingolipids in a variety of important biological processes. Such processes include signaling events related to cell growth, differentiation, programmed cell death, and stress responses. These processes not only involve those sphingolipids that accumulate as a result of a variety of inherited lysosomal storage disorders, but, in addition, sphingolipids associated with long-chain base metabolism. This article reviews the chemical properties, pathways, regulated metabolism, and signaling function of sphingolipids. In addition, the potential roles of sphingolipids in renal-specific processes are considered. While a variety of cellular functions have been ascribed to sphingolipids, in many cases proof of the concept has yet to be well established. Thus, a number of critical questions can be posed in interpreting these studies. Several of these questions are considered.

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Interleukin-1-mediated PGE2 production and sphingomyelin metabolism. Evidence for the regulation of cyclooxygenase gene expression by sphingosine and ceramide.

Cited by 258 publications

References 36 publications

Role of the ceramide-signaling pathway in cytokine responses to P-fimbriated Escherichia coli.

Role of the ceramide-signaling pathway in cytokine responses to P-fimbriated Escherichia coli.

Novel ceramides recovered from Porphyromonas gingivalis: relationship to adult periodontitis

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