Interleukin-10 and Interferon Gamma Gene Polymorphisms and Hepatitis C Virus–Related Liver Cirrhosis Risk

Abstract

The aim of the study was to evaluate the association between the gene polymorphisms in interleukin-10 (IL-10) and interferon gamma (IFN-γ) genes with susceptibility and severity of hepatitis C virus (HCV) infection among Egyptian patients. Interleukin-10 -592 A/C, -1082 G/A and IFN-γ +874 T/A genotypes were determined in 100 chronic HCV patients and 50 healthy controls using restriction fragment length polymorphism (RFLP) and the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) respectively. IL-10 -592 A/C polymorphism genotyping revealed that the frequency of CC genotype was significantly higher in chronic HCV patients than in controls (58% versus 30%, P < 0.05). Regarding IL-10 -1082 G/A polymorphism genotyping, a higher frequency of GG genotype was found in chronic HCV patients compared to controls (31% versus 10%, P < 0.05). IFN-γ +874 T/A genotyping showed that TT genotype was significantly higher in chronic HCV participants than controls (31% versus 18%, P < 0.05), while a higher frequency of T allele was found in cirrhotic patients compared to noncirrhotic patients (P < 0.05). Our observations suggested that IL-10 -592 A/C, -1082 G/A, and IFN-γ +874 T/A polymorphisms had a strong association with susceptibility to HCV infection. However, no significant association was observed between the cytokines (IL-10 and IFN-γ) genotypes profile and HCV-liver cirrhosis risk in the studied population, except for the high frequency of IFN-γ +874 T allele in cirrhotic patients.

TNFA -308G>A and IL10 -1082A>G polymorphisms seem to be predictive biomarkers of chronic HCV infection

“…The TNFA -308G>A and IL10 -1082A>G polymorphisms were not associated with different levels of necroinflammatory activity or with fibrosis score. Several studies have also found no relationship between these polymorphisms in the TNF and IL10 genes and different stages of the disease [ 15 , 16 , 24 , 28 , 33 ]; probably because the disease progression is due to other factors and not only to the host genetic profile.…”

TNFA -308G&gt;A and IL10 -1082A&gt;G Polymorphisms as Predictive Biomarkers of Chronic HCV Infection

“…The TNFA − 308G > A and IL10 -1082A > G polymorphisms were not associated with different levels of necroin ammatory activity or with brosis score. Several studies have also found no relationship between these polymorphisms in the TNF and IL10 genes and different stages of the disease [21,25,28,31,32). Thus, these polymorphisms seem not to in uence the progression of the histopathological processes of chronic HCV infection because in this disease, in addition to the host immunological factors, others factors inherent to the virus act directly on the in ammatory process, causing tissue damage.…”

The TNFA -308G&gt;A and IL10 -1082A&gt;G Polymorphisms as Predictive Biomarkers of Chronic HCV Infection

“…The TNFA − 308G > A and IL10 -1082A > G polymorphisms were not associated with different levels of necroin ammatory activity or with brosis score. Several studies have also found no relationship between these polymorphisms in the TNF and IL10 genes and different stages of the disease [21,25,29,30]. Thus, these polymorphisms seem not to in uence the progression of the histopathological processes of chronic HCV infection because in this disease, in addition to the host immunological factors, others factors inherent to the virus act directly on the in ammatory process, causing tissue damage.…”