Interleukin-10 and interferon-gamma patterns during tuberculosis treatment: possible association with recurrence [Short communication]

Lago, Patrícia M.; Boéchat, Neio; Migueis, Débora Petrungaro; Almeida, Alexandre S. de; Lazzarini, Luiz Claudio Oliveira; Saldanha, May; Kritski, Afrânio Lineu; Ho, John L.; Silva, José Roberto Lapa e

doi:10.5588/ijtld.11.0707

Cited by 17 publications

(16 citation statements)

References 8 publications

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“…This distinction also manifests in the two APTB patients with treatment failure, who recorded low INF-y/IL-10 ratio at baseline and fluctuating but consistently low ratios at the different time points during their unsuccessful TB treatment. The persistently elevated IL-10 responses noted in the APTB patients and, particularly, in the two with unresolved TB correlates with the previously reported association of high IL-10 production in pulmonary TB patients with MDR-TB [24], and in those with possible TB recurrence [33]. Taken together, these observations indicate that an altered balance between the pro-(INF-y) and anti-(IL-10) inflammatory cytokine is implicated in MTb infection progression and possibly resistance, rather than elevation or decrease of just one of the cytokines.…”

Section: Discussionsupporting

confidence: 87%

The Patterns of Interferon-Gamma and Interleukin-10 Production as a Potential Immunological Biomarker for the Outcome of Mycobacterium Tuberculosis Infection

Akinshipe¹

2017

IJI

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Deficiencies in current tuberculosis (TB) immunodiagnostics pipeline demand new approaches to control TB.Because the balance in key pro-and anti-inflammatory cytokines production could determine Mycobacterium Tuberculosis (MTb) infection outcome, this study aimed to determine the patterns of MTb-specific antigen-stimulated Interferon-gamma (IFN-y) and Interleukin-10 (IL-10) production in different clinical forms of MTb infection and to evaluate their concomitant changes during anti-TB treatment (ATT). Overall, 84 BCG-vaccinated HIV-negative adults, consisting of 25 Healthy Community Controls (HCC), 27 Latent Tuberculosis Infection (LTBI) cases, and a cohort of 32 Acute Pulmonary Tuberculosis (APTB) patients were investigated for IFN-y and IL-10 responses at enrollment (base-line) and during ATT at 2-month (ATT1) and 6-month (ATT2). At enrollment, groups didn't differ significantly in age, gender, or CD4+ T counts but differed in the other socio-demographics, and hematological parameters, p<0.05. Base-line Sandwich ELISA -measured IFN-y responses were significantly higher in HCC (223.50±58.11pg/ml) compared with LTBI (128.82±41.81pg/ml) and APTB (47.82±22.05pg/ml), p<0.0001 in each case. During treatment, IFN-y levels increased significantly at ATT1 (125.37±16.09pg/ml) and ATT2 (203.35±23.24pg/ml), p<0.0001. Conversely, base-line IL-10 responses increased significantly in APTB (17.53±6.30pg/ml), compared with LTBI (10.71±2.39pg/ml) and HCC (7.49±2.02pg/ml), P<0.0001, but declined significantly at ATT1 (10.54±2.25pg/ml) and ATT2 (5.25±1.45pg/ml), P=<0.0001. Cytokines response combination ratio showed: 'High' HCC, 'Intermediate' LTBI, or 'Low' APTB ratio that increased during successful ATT; the two identified MDR-TB patients recorded fluctuating but constantly low ratio during ATT. These results demonstrate the immunocompetence of MTb-exposed adults, and that IFN-y and IL-10 cytokines cross-regulate, and strongly suggest a shift toward IFN-ymediated pro-inflammatory host immune phenotype during effective control of MTb infection. The IFN-y/IL-10 response ratio is a novel potential immunological biomarker to assess if MTb infection is going to resolve, result in latency, progress to TB; or become drug-resistant.

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Section: Discussionsupporting

confidence: 87%

The Patterns of Interferon-Gamma and Interleukin-10 Production as a Potential Immunological Biomarker for the Outcome of Mycobacterium Tuberculosis Infection

Akinshipe¹

2017

IJI

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“…Another study has recently shown a consistent decrease in IL-10 levels in active TB patients at all-time points of therapy, suggesting that patients who maintain high IL-10 levels at the end of treatment are exposed to TB recurrence [ 12 ]. Sahiratmadja et al also observed reduced IL-10 production during TB therapy, suggesting that this cytokine may be a useful biomarker signature to assess the disease progression [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Interleukin-10 Levels in the Plasma of Patients With Various Stages of Tuberculosis

Ndishimye

Seghrouchni

Domokos

et al. 2015

Medicine and Pharmacy Reports

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Mycobacterium tuberculosis (Mtb) infection remains one of the world’s major causes of illness and mortality. A clear understanding of the host defense against Mtb is imperatively needed forthe control of this epidemic. When tuberculosis (TB) infection occurs, a variety of pro and anti-inflammatory cytokines play a vital role in the pathogenesis of this disease. Interleukin-10 (IL-10) is one of the most important anti-inflammatory cytokines reported to suppress the protective immune response against tuberculosis.AimThe aim of the present study was to evaluate the association of plasma IL-10 levels with various disease stages of TB and the possible effects of treatment on these levels.Materials and methodsA group of 30 patients with active pulmonary TB and a control group of 21 healthy individuals were enrolled in this study. The levels of IL-10 were measured before, during, and after treatment using commercially available enzyme-linked immune-sorbent assay (ELISA). Data were analyzed using GraphPad Prism version 5.0.ResultsThe results showed that the levels of IL-10 had significant differences between the TB and control groups (p<0.05). The patients with abnormal chest X-Ray findings had higher IL-10 levels when compared to patients with normal X-Rays (p=0.03). A subgroup of 18 patients were followed during the treatment and the mean plasma concentration of IL-10 in patients before therapy was higher than in patients at 3 months of therapy and in patients after 6 months of therapy (p=0.01). However, the IL-10 level remained significantly higher in patients at the end of treatment compared with controls.These findings could be used in follow-up as clinical biomarker of the success of tuberculosis therapy.

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“…It strongly promotes the T helper 1 (Th1) cell response. Moreover, it has been demonstrated to act as a protective factor against tuberculosis in human and animal studies [35], [36]. INF-γ participates in the pathogenesis of inflammation in chronic obstructive pulmonary disease [37].…”

Section: Discussionmentioning

confidence: 99%

Increased Cytokines Response in Patients with Tuberculosis Complicated with Chronic Obstructive Pulmonary Disease

et al. 2013

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ObjectivesTo explore the change and its significance of cytokines in patients with pulmonary tuberculosis complicated with COPD.MethodsThe immune function of 152 cases of pulmonary tuberculosis with COPD was detected to compare with 150 cases of patients with pulmonary tuberculosis, 157 cases of patients with COPD and 50 cases of healthy volunteers who were in the hospital during the same period. T lymphocyte cell population in peripheral blood was detected by flow cytometry. The serum levels of sIL-2R, IL-6, IFN-γ, TNF-α were measured using ELISA.ResultsThe percentage of CD4+ T cells in TB patients with or without COPD and COPD patients without TB was significantly lower than that in control group. The percentage of CD4+ T cells in patients with TB and COPD was significantly lower than that in the non-COPD TB patients. The percentage of CD8+ T cells was higher in the TB patients group than that in control group. The CD4+/CD8+ ratio in the TB patients group was significantly lower than that in control group. The concentrations of sIL-2R, IL-6, TNF-α, IFN-γ in TB patients with or without COPD and COPD patients without TB were significantly higher than those in control group. In addition, sIL-2R, IL-6, TNF-α concentrations in the patients with TB and COPD were higher than those in the non-COPD TB patients. The concentrations of sIL-2R, IL-6, TNF-α, IFN-γ in COPD patients with TB were significantly higher than those in COPD patients without TB. There was a significant negative correlation between serum levels of TNF-α, IL-6 and FEV1 (%, predicted) in COPD without TB group.ConclusionsThe patients with pulmonary tuberculosis complicated with COPD were impaired in cellular immunity, and its extent of immune impairment is more serious than those of the patients with pulmonary tuberculosis and the patients with COPD.

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Interleukin-10 and interferon-gamma patterns during tuberculosis treatment: possible association with recurrence [Short communication]

Cited by 17 publications

References 8 publications

The Patterns of Interferon-Gamma and Interleukin-10 Production as a Potential Immunological Biomarker for the Outcome of Mycobacterium Tuberculosis Infection

The Patterns of Interferon-Gamma and Interleukin-10 Production as a Potential Immunological Biomarker for the Outcome of Mycobacterium Tuberculosis Infection

Evaluation of Interleukin-10 Levels in the Plasma of Patients With Various Stages of Tuberculosis

Increased Cytokines Response in Patients with Tuberculosis Complicated with Chronic Obstructive Pulmonary Disease

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