Interleukin 10 is a human T cell growth factor in vitro

Pawelec, Graham; Pohla, Heike; Schlotz, Elke; Rehbein, Arnika; Schaudt, Kurt; Hambrecht, Andreas; Friccius, Hilke

doi:10.1006/cyto.1995.0045

Cited by 16 publications

(7 citation statements)

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“…(19) Our present results showed that IL-10 can be a growth factor for T cells that remain after intensive chemotherapy. Although IL-10-dependent proliferation of preactivated CTC was observed for many clones, the effects of IL-10 on PHA-stimulated proliferation were divergent and seemed to depend both on clonal characteristics and on differences between AML blasts derived from various patients.…”

Section: Discussionsupporting

confidence: 60%

Cytokine Responsiveness of Mitogen-Activated T Cells Derived from Acute Leukemia Patients with Chemotherapy-Induced Leukopenia

Bruserud

Ulvestad

2000

Journal of Interferon & Cytokine Research

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The aim of the study was to characterize effects of exogenous cytokines on T lymphocytes derived from acute leukemia patients with chemotherapy-induced leukopenia. We investigated the cytokine responsiveness of long-term expanded CD4+ and CD8+ T cell clones and the effects of exogenous cytokines on anti-CD3-stimulated polyclonal T cell responses. After mitogenic activation in the presence of acute myelogenous leukemia (AML) blasts, most CD4+ and CD8+ clones proliferated in response to interleukin-2 (IL-2). Although a majority of the IL-2-responsive clones could also proliferate in the presence of exogenous IL-4, IL-7, IL-9, IL-10, IL-12, and IL-15, only IL-15 responses were equal to or exceeded the corresponding IL-2 responses. Exogenous cytokines were also added during T cell activation with phytohemagglutinin (PHA) + accessory leukemia cells derived from different AML patients, and all the cytokines then had divergent effects that depended on both differences between clones and differences between AML patients. However, for most of these T cell clone/AML blast combinations, IL-2 and IL-15 caused enhanced T cell proliferation. IL-2 and IL-15 also enhanced anti-CD3-stimulated polyclonal responses of nonexpanded T cells derived from cytopenic patients, whereas other cytokines had only minor effects. Our results demonstrate that cytokine-responsive T cells remain in the circulation during chemotherapy-induced cytopenia, and combination therapy including intensive chemotherapy and T cell-targeting cytokine therapy should, therefore, be possible in AML.

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Section: Discussionsupporting

confidence: 60%

Cytokine Responsiveness of Mitogen-Activated T Cells Derived from Acute Leukemia Patients with Chemotherapy-Induced Leukopenia

Bruserud

Ulvestad

2000

Journal of Interferon & Cytokine Research

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“…However, IL‐10 may also have an inflammatory function by activating B cells and thereby promoting the production of autoantibodies and, under IL‐2 deprivation, inhibiting apoptosis of T cells and supporting the expansion of T‐cell clones (Taga et al. , 1993; Pawelec et al. , 1995).…”

Section: Introductionmentioning

confidence: 99%

“…It has an anti-inflammatory, regulatory effect on immune responses by decreasing the production of several immunoactive molecules, such as tumour necrosis factor alpha (TNF-a), interferon (IFN)-c, IL-12, reactive nitric oxide metabolites, major histocompatibility complex molecules (de Waal-Malefyt et al, 1991;Moore et al, 1993;Eskdale et al, 1998) and by inhibiting antigen-specific cytotoxic T cells (Bejarano et al, 1992). However, IL-10 may also have an inflammatory function by activating B cells and thereby promoting the production of autoantibodies and, under IL-2 deprivation, inhibiting apoptosis of T cells and supporting the expansion of T-cell clones (Taga et al, 1993;Pawelec et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

IL‐10 promoter haplotype influence on interferon treatment response in multiple sclerosis

Wergeland

Beiske

Nyland

et al. 2005

Euro J of Neurology

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The level of interleukin-10 (IL-10) expression is related to polymorphisms -1082 (G/A), -819 (T/C) and -592 (A/C) in the promoter region of the IL-10 gene, which constitute three haplotypes, GCC, ATA, and ACC. The ATA (a non-GCC) haplotype, which is associated with low IL-10 expression, has been shown to improve interferon (IFN) treatment response in hepatitis C. We analysed the distribution of IL-10 promoter haplotype combinations to determine whether they could influence initial IFN treatment response in 63 patients with relapsing-remitting multiple sclerosis (MS). The patients were grouped into non-GCC or GCC haplotypes, and the clinical and magnetic resonance imaging (MRI) disease activity was compared in the two groups. During the first 6 months of treatment, MS patients with non-GCC haplotypes experienced fewer new MRI T1-contrast enhancing lesions [0.77+/-0.36 (SEM)] than patients with the GCC haplotype (2.45+/-0.57) (P=0.05, Mann-Whitney U test). No differences were detected on clinical disease activity. The results suggest an influence of IL-10 promoter polymorphisms on IFN treatment response in MS.

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“…The majority also responded to IL-15. Responses to IL-4 were completely lacking in clones derived without either IL-7 or OM, whereas none of the clones from any culture protocol responded to IL-9 or IL-12 (recognized TCGFs) or to IL-10 (which can sometimes appear to act as a TCGF by virtue of its anti-apoptotic action [26]). Further studies of cytokine receptor expression, and the influence on this of the cytokines initially present, may help to elucidate such differential cytokine reactivities.…”

Section: Discussionmentioning

confidence: 97%

Extrathymic T cell differentiation in vitro from human CD34+ stem cells

Pawelec

Müller

Rehbein

et al. 1998

Journal of Leukocyte Biology

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Although it is well established that T cells are derived from CD34 ؉ stem cells in vivo, and that T cells can develop in the absence of a functioning thymus, it has not proven possible thus far to generate human T cells in vitro from CD34 ؉ cells in the absence of any thymic influence. We now present a limiting dilution cloning culture system that supports the differentiation of highly purified human CD34 ؉ cells to CD3 ؉ T cells in vitro in the complete absence of any thymic components. The culture system features the use of a serum-free medium supplemented with a cocktail of cytokines including flt-3 ligand, interleukin-3 (IL-3), stem cell factor (SCF), and IL-2. CD4 ؉ T cell clones capable of mitogen-stimulated proliferation and response to IL-2, and expressing a varied TCR-V␤ repertoire were obtained under these conditions. This culture system therefore supports human T lymphopoiesis in the absence of any thymic influence and may prove useful for the evaluation of extrathymic T cell differentiation in vitro. J. Leukoc. Biol. 64: 733-739; 1998.

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Interleukin 10 is a human T cell growth factor in vitro

Cited by 16 publications

References 0 publications

Cytokine Responsiveness of Mitogen-Activated T Cells Derived from Acute Leukemia Patients with Chemotherapy-Induced Leukopenia

Cytokine Responsiveness of Mitogen-Activated T Cells Derived from Acute Leukemia Patients with Chemotherapy-Induced Leukopenia

IL‐10 promoter haplotype influence on interferon treatment response in multiple sclerosis

Extrathymic T cell differentiation in vitro from human CD34+ stem cells

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