Interleukin 10 reduces the severity of acute pancreatitis in rats

Rongione, Anthony J.; Kusske, Amy M.; Kwan, Karen; Ashley, Stanley W.; Reber, Howard A.; McFadden, David W.

doi:10.1053/gast.1997.v112.pm9041259

Cited by 271 publications

(166 citation statements)

References 3 publications

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“…Various proinflammatory cytokines, such as interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α), appear to play a major role in acute pancreatitis [2,3] . The inhibition of cytokine production may decrease the severity of pancreatitis [4,5] . It was recently reported that peroxisome proliferatoractivated receptors (PPARs) play a modulatory role in the inflammatory response of different organs [6] .…”

Section: Introductionmentioning

confidence: 99%

Pioglitazone attenuates the severity of sodium taurocholate-induced severe acute pancreatitis

Xu¹

2007

WJG

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AIM:To determine the effect of pioglitazone, a specific peroxisome proliferator-activated receptor-γ (PPARγ) ligand, on development of severe acute pancreatitis (SAP) and expression of nuclear factor-kappa B (NF-κB) and intercellular adhesion molecule-1 (ICAM-1) in the pancreas. METHODS:Male Sprague-Dawley (SD) rats (160-200 g) were randomly allocated into three groups (n = 18 in each group): severe acute pancreatitis group, pioglitazone group, sham group. SAP was induced by retrograde infusion of 1 mL/kg body weight 5% sodium taurocholate (STC) into the biliopancreatic duct of male SD rats. Pioglitazone was injected intraperitoneally two hours piror to STC infusion. Blood and ascites were obtained for detecting amylase and ascitic capacity. Pancreatic wet/dry weight ratio, expression of NF-κB and ICAM-1 in pancreatic tissues were detected by immunohistochemical staining. Pancreatic tissue samples were stained with hematoxylin and eosin (HE) for routine optic microscopy. RESULTS:Sham group displayed normal pancreatic structure. SAP group showed diffuse hemorrhage, necrosis and severe edema in focal areas of pancreas. There was obvious adipo-saponification in abdominal cavity. Characteristics such as pancreatic hemorrhage, necrosis, severe edema and adipo-saponification were found in pioglitazone group, but the levels of those injuries were lower in pioglitazone group than those in SAP group. The wet/dry pancreatic weight ratio, ascetic capacity, serum and ascitic activities of anylase in the SAP group were significantly higher than those in the sham group and pioglitazone group respectively (6969.50 ± 1368.99 vs 2104.67 ± 377.16, 3.99 ± 1.22 vs 2.48 ± 0.74, P < 0.01 or P < 0.05). According to Kusske criteria, the pancreatic histologic score showed that interstitial edema, inflammatory infiltration, parenchyma necrosis and parenchyma hommorrhage in SAP group significantly differed from those in the sham group and pioglitazone group (7.17 ± 1.83 vs 0.50 ± 0.55, 7.67 ± 0.82 vs 6.83 ± 0.75, P < 0.01, P < 0.05.The expression of NF-κB and ICAM-1 in sham group was lower than that in SAP group and pioglitazone group (0.50 ± 0.55 vs 33 ± 1.21, P < 0.01). There was a significant difference in the expression of NF-κB and ICAM-1 between SAP group and pioglitazone group (7.50 ± 1.05 vs 11.33 ± 1.75, 0.80 ± 0.53 vs 1.36 ± 0.54, P < 0.01 or P < 0.05) at 12 h after the induction of pancreatitis. CONCLUSION:

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Section: Introductionmentioning

confidence: 99%

Pioglitazone attenuates the severity of sodium taurocholate-induced severe acute pancreatitis

Xu¹

2007

WJG

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“…The stained sections were evaluated by a pathologist who was uninformed about the groups. The method of Rongione et al, (1997) was used for scoring the degree of oedema, inflammation, vacuolization, necrosis in the pancreas.…”

Section: Methodsmentioning

confidence: 99%

The Combined Effects of Etanercept Plus Methylprednisolone on Pancreatic Oxidative Stress in Acute Pancreatitis Induced by Cerulein

Günay¹,

Kılıç²,

AMANVERMEZ³

2011

jecm

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KeywordsEtanercept Methylprednisolone Acute pancreatitis Oxidative stress Malondialdehyde Protein carbonyls ARTICLE INFO ABSTRACTWhether etanercept plus metylprednisolone reduce pancreatic oxidative stress and injury during pancreatitis is unknown well. The aim of this study was to examine the therapeutic effects of etanercept and methylprednisolone (MP) on acute pancreatitis and oxidative stress in acute pancreatitis (AP) induced by cerulein in a rat model. The study was performed with 48 rats divided into 6 groups (n = 8/group): 1-sham, 2-cerulein-induced pancreatitis (over 10 hours), 3-cerulein-pancreatitis (over 20 hours), 4-etanercept (5 mg/ kg, i.p.), 5-methylprednisolone (10 mg/kg, i.m.), 6-etanercept plus methylprednisolone. Also, the rats in groups 4, 5, and 6 were cerulein-induced pancreatitis at 20 hours. After the treatment, the pancreas and blood were taken for histopathological and biochemical analysis. All cerulein-treated rats developed biochemical and pathological acute pancreatitis after 10-20 hours. The markers of oxidative stress such as protein carbonyls, lipid peroxidation, and myeloperoxidase (mpo) in the pancreas tissues were increased in the group 2 and 3, but these were lower in etanercept and MP-treated rats compared to groups 3. Pancreatic mpo activity was considerably reduced in pancreas tissues at 20 h after the administration of etanercept plus metylprednisolone in the group 6 compared to group 3. In AP induced by cerulein, the treatment of etanercept plus methylprednisolone may ameliorate pancreatic oxidative stress in rats.

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“…IL-1 , IL-6 and TNF-) from monocytes/macrophages thus preventing subsequent tissue damage. IL-10 also stimulates production of naturally occurring IL-1 receptor antagonist (IL1ra) and release of soluble p75 TNF receptor [108]. IL-10 is believed to have a protective role in acute pancreatitis.…”

Section: Il-10mentioning

confidence: 99%

“…IL-10 is believed to have a protective role in acute pancreatitis. Administration of IL-10 in experimental acute pancreatitis reduces the local inflammatory response and subsequent mortality [108,109]. …”

Section: Il-10mentioning

confidence: 99%

Microcirculatory Disturbances in the Pathogenesis of Acute Pancreatitis

Uhlmann¹

2012

Acute Pancreatitis

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Interleukin 10 reduces the severity of acute pancreatitis in rats

Cited by 271 publications

References 3 publications

Pioglitazone attenuates the severity of sodium taurocholate-induced severe acute pancreatitis

Pioglitazone attenuates the severity of sodium taurocholate-induced severe acute pancreatitis

The Combined Effects of Etanercept Plus Methylprednisolone on Pancreatic Oxidative Stress in Acute Pancreatitis Induced by Cerulein

Microcirculatory Disturbances in the Pathogenesis of Acute Pancreatitis

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