Aim: To study the effects of 1,8‐cineol (eucalyptol) on the expression of early growth response factor‐1 (Egr‐1) and NF‐κB in the human monocyte THP‐1 cell line stimulated by lipopolysaccharide (LPS). Methods: The THP‐1 cells were incubated with serial doses of 1,8‐cineol (1, 10, and 100 mg/L, 30 min) before being stimulated with LPS (1 mg/L, 30 min). The localization of Egr‐1 in the THP‐1 cells was detected by immunofluorescence and a laser scanning confocal microscope. The expression of Egr‐1 in the nuclei and whole cell, and NF‐κB in the nuclei, were measured by Western blot analysis. Results: When stimulated by LPS, the FITC‐labeled Egr‐1 was detected mainly in the nuclei. Moreover, the expression of Egr‐1 in the whole cell increased markedly compared with the control cells. 1,8‐Cineol pretreatment decreased the expression of Egr‐1 in both the nuclei and whole cell of the LPS‐stimulated THP‐1 cells, and this effect was concentration‐dependent, but there was no reaction on the expression of NF‐κB in the nuclei protein in the LPS‐stimulated THP‐1 cells. Conclusion: In a concentration‐dependent manner, 1,8‐Cineol reduces LPS‐induced Egr‐1 expression in nuclei and in whole cell of THP‐1 cells, but shows no effect on NF‐κB expression.