Interleukin-12 Gene Modification Exerts Anti-Tumor Effects on Murine Mammary Sarcoma Cell Line in vivo

Li, Dan; Yu, Hong; Xu, Teng; Li, Jing Hua; Sun, Yun Fang; Zhang, Wen Qing

doi:10.1038/cmi.2008.28

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…The M. phlei cell wall complex directly induces apoptosis of human bladder cancer cells by producing IL-12 (19). In addition, IL-12 upregulates production of other cytokines, including IFN-γ and TNF-α (21). According to a report by Bera et al (10), heat-killed M. phlei plays a beneficial role as an immunostimulant against cecal coccidiosis in broiler chickens.…”

Section: Discussionmentioning

confidence: 99%

Effects of Mycobacterium phlei on intestinal microecology andserum immunological parameters of weaned piglets

Zhong¹,

Zhang²,

Wu³

et al. 2016

Turk J Vet Anim Sci

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The aim of this study was to determine the effects of Mycobacterium phlei in diets (Experiment 1) and investigate the mode of action on the intestinal microecology of weaned piglets (Experiments 1 and 2). In Experiment 1, a total of 96 commercial cross-bred pigs [(Duroc × Yorkshire) × Landrace], weaned at 21 days, were assigned to two treatments in a completely randomized design with a single factorial arrangement of 40 days of supplementation (nonsupplemented vs. supplemented with 0.4% heat-killed M. phlei). The results showed that M. phlei has the capability to improve the intestinal microecology of weaned piglets. The serum concentrations of IL-2, IL-12, and TNF-α from the experimental group were significantly higher than in the control group (P < 0.01). In Experiment 2, the antibacterial effect of 5 different extracts (pure water, 95% ethanol, chloroform, ethyl acetate, and n-butanol) from M. phlei was assayed by the Plat stiletto method. The results showed that these extracts had hardly any antibacterial effect on E. coli. Our results indicate that M. phlei can improve the intestinal microecology of weaned piglets not because of its antibacterial effects, but because of these immune factors stimulated by the microecology of weaned piglets.

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Section: Discussionmentioning

confidence: 99%

Effects of Mycobacterium phlei on intestinal microecology andserum immunological parameters of weaned piglets

Zhong¹,

Zhang²,

Wu³

et al. 2016

Turk J Vet Anim Sci

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show abstract

“…In general, an increase in IL-12, CD8+ T-cells, IFN-γ, serum IgG2a, or NK cells suggests an increase in the Th1 immune response and indicates the induction of antitumor effects [ 6 , 9 , 11 , 18 , 19 ]. Although IL-12 induction has an anti-tumorigenic effect through early innate immune responses, further cellular immunity is essential for some immune synergy in the reduction of tumor growth.…”

Section: Discussionmentioning

confidence: 99%

Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment

et al. 2014

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Toxoplasma gondii is an intracellular protozoan parasite that causes a Th1 cellular immunity. Our previous study showed that T. gondii lysate antigen (TLA) treatment in S180 tumor-bearing mice resulted in tumor reduction by suppressing CD31 expression, a marker of angiogenesis. In the present study, to investigate tumor suppressive effect of TLA under the absence of T lymphocytes, athymic nude mice were compared with euthymic mice in the anti-tumorigenic effect triggered by TLA in CT26 tumors. According to the results, intratumorally injected TLA reduced tumor growth and TIMP-1 level, a metastatic marker, in both euthymic and athymic mice. TLA treatment led to a sharp increase in IL-12 expression in serum cytokine profiling of athymic mice, and increased MyD88 signals in macrophages derived from the bone marrow, implying the activation of innate immunity. The selective induction of IL-12 by TLA treatment had an anti-tumorigenic effect.

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“…IL-12, a multifunctional cytokine, has been shown to possess potent antitumour activity in a variety of murine tumour models (Imagawa et al 2004;Li et al 2008;Pavlin et al 2009). However, systemic application of IL-12 in the clinic is hindered by its severe toxicity (Cohen 1995;Leonard et al 1997;Car et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Clostridium sporogenes delivers interleukin-12 to hypoxic tumours, producing antitumour activity without significant toxicity

Zhang

Lü

Chang

et al. 2014

Lett Appl Microbiol

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Interleukin-12 (IL-12) is a potent antitumour cytokine, but it is toxic when administrated systemically. This study demonstrates that murine IL-12 can be systemically delivered to hypoxic sites in solid tumours by Clostridium sporogenes, producing a clear delay in tumour growth and a 14·3% cure rate in a mouse tumour model. Importantly, there is no obvious toxicity associated with IL-12 during the treatment process. This result may be accounted for by the excellent tumour-targeting capacity of Cl. sporogenes, targeting IL-12 directly to the tumour site instead of to the entire body.

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Interleukin-12 Gene Modification Exerts Anti-Tumor Effects on Murine Mammary Sarcoma Cell Line in vivo

Cited by 4 publications

References 7 publications

Effects of Mycobacterium phlei on intestinal microecology andserum immunological parameters of weaned piglets

Effects of Mycobacterium phlei on intestinal microecology andserum immunological parameters of weaned piglets

Prominent IL-12 Production and Tumor Reduction in Athymic Nude Mice after Toxoplasma gondii Lysate Antigen Treatment

Clostridium sporogenes delivers interleukin-12 to hypoxic tumours, producing antitumour activity without significant toxicity

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