Interleukin‐12 regulates natural killer cell‐dependent Propionibacterium acnes‐primed, lipopolysaccharide‐induced liver injury

Abstract

IL-12-regulated IFN-gamma production is crucial during the priming phase by P. acnes, but not at the time of the subsequent LPS challenge. NK1.1(+)CD3(-)CD4(-) NK or NK1.1(+)CD3(+)CD4(-) NKT cells are important in this model of liver injury.

Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein

“…As cytokines are the major effectors and regulators of the immune response, we next evaluated the ability of spleen cells to produce IFN-γ and IL-4 that are considered key cytokines in the development of Th1 and Th2 cells, respectively [ 23 ]. As IFN-γ can be directly induced by polyclonal activation of T cells, the spleen cells were stimulated with ConA, LPS and SAC were additionally used because they indirectly induce IFN-γ production by NK cells, i.e, via IL-12 production [ 24 , 25 ]. In ConA stimulated cultures there was no difference between normal and dietary restricted groups.…”