Interleukin-15 Is an Autocrine/Paracrine Viability Factor for Cutaneous T-Cell Lymphoma Cells

Döbbeling, Udo; Dummer, Reinhard; Laine, Elisabeth; Potoczna, Natascha; Qin, Jian‐Zhong; Burg, Günter

doi:10.1182/blood.v92.1.252.413k08_252_258

Cited by 151 publications

(83 citation statements)

References 29 publications

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“…We describe here the establishment of an animal model for MF. The cell line MyLa, used in our study, is derived from a tumor biopsy of an MF patient and grows cytokine independent under tissue culture conditions (9). Subcutaneous transplantation of MyLa cells generated large subcutaneous tumors in immune deficient nu/nu mice.…”

Section: Discussionmentioning

confidence: 99%

“…The lymphocytes were purified from spleen or lymph nodes. Cells were expanded in tissue culture for 3 weeks without the addition of cytokines, because MyLa cells are able to grow cytokine independently (9). Cells derived from blood, lymph node, and spleen expressed the human CD4 receptor, grew independently of interleukins and were thereby identified as MyLa cells (data not shown).…”

Section: Pcr Analysis Of the Human Cd4 Receptor (Data Not Shown) A Cmentioning

confidence: 99%

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Establishment of a mouse xenograft model for mycosis fungoides

Thaler

Burger

Schulz

et al. 2004

Experimental Dermatology

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Mycosis fungoides (MF) is the most frequent variant of cutaneous T-cell lymphomas (CTCLs). MF primarily involves the skin initially with patches and plaques. In later stages, cutaneous tumors develop and tumor cells may spread to lymph nodes and finally to visceral sites. Here, we describe an animal model for MF in immune-deficient nude mice, using the CTCL cell line MyLa. Subcutaneous transplantation of MyLa cells leads to the formation of cutaneous tumors in 80% of the mice (50/60 total). Spread of tumor cells to visceral sites was detected by immunohistochemistry and polymerase chain reaction (PCR)-based detection of specific T-cell receptor-gamma rearrangement. MyLa cells were found circulating in the blood, lymph nodes, and in blood vessels of heart, kidney, lung, and liver. In lung and liver tissue, tumor cells presented perivascular invasion, but no large secondary tumors developed. The nude mouse model described here will be a valuable test system for new therapeutic approaches for the treatment of MF and opens the unique opportunity to study the disease in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Pcr Analysis Of the Human Cd4 Receptor (Data Not Shown) A Cmentioning

confidence: 99%

Establishment of a mouse xenograft model for mycosis fungoides

Thaler

Burger

Schulz

et al. 2004

Experimental Dermatology

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“…IL-15 [41] und IL-7 [28] sind potente Wachstumsfaktoren für CTCL-Zelllinien und verlängern in vitro die Überlebenszeit von CTCL-Zellen von Patienten mit Sézary-Syndrom. IL-15 wird in Keratinozyten der Basalschicht und dendritischen Hautzellen exprimiert [29], jedoch ebenso von neoplastischen Lymphozyten [30], was zu einer autokrinen Schleife der Zellstimulation führt. Neue Ansätze zur Therapie können sich aus der Modulation von Homing-Rezeptoren ergeben.…”

Section: Infektiöse Faktorenunclassified

Cutaneous malignant lymphomas: Update 2006

Burg

Kempf

Cozzio

et al. 2006

J Deutsche Derma Gesell

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Cutaneous lymphomas represent a unique group of lymphomas and are the second most frequent extranodal lymphomas. As with other neoplasias, the pathogenesis is based mainly on a stepwise accumulation of mutations of suppressor genes and oncogenes caused by genetic, environmental or infectious factors. The diagnostic work-up includes clinical, histological, imaging and hematological investigations and in many cases immunohistochemical and molecular biological analyses. The current WHO/EORTC classification of cutaneous lymphomas differentiates "mature T-cell and NK-cell lymphomas", "mature B-cell lymphomas" and "immature hematopoietic malignancies", their variants and subgroups. It is compatible with the WHO classification for neoplasias of the hematopoietic and lymphoid tissue and respects the organ-specific peculiarities of primary cutaneous lymphomas. The assignment of the various types of cutaneous lymphomas into prognostic categories (pre-lymphomatous "abortive" disorders; definite malignant lymphomas of low-grade malignancy; definite malignant lymphomas of high-grade malignancy) provides essential information on the biological behavior and allows an appropriate planning of the therapeutic strategy, which may be topical or systemic and aggressive or non-aggressive. Besides the classical options for therapy, there are new and "experimental" strategies, the efficacy of which has to be studied in clinical trials.

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“…IL-2, IL-7, IL-9, IL-15, IL-21). [37][38][39][40] Consequently, blood eosinophil counts and IgE-t are frequently increased in SS and advanced MF. Tancr ede-Bohin et al 41 reported that blood eosinophilia (>700 eosinophils/lL) was present in 10 of 13 (77%) patients with SS.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of atopy and staphylococcal superantigen‐specific immunoglobulin E (IgE) antibodies and total serum IgE in primary cutaneous T‐ and B‐cell lymphoma

Vonderheid

Hamilton

Kadin

2019

The Journal of Dermatology

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The prevalence of atopy was investigated in 20 patients with Sézary syndrome (SS), 20 patients with plaque phase mycosis fungoides (MF), 9 patients with primary cutaneous marginal zone lymphoma (pcMZL) and 8 patients with primary cutaneous follicle center lymphoma (pcFCL) with the Phadiatop multi‐allergen test. The relationship among serologic atopy, IgE reactivity against Staphyloccocal enterotoxin superantigens, and serum total IgE (IgE‐t) levels and their prognostic implications in SS was investigated. Phadiatop test was positive in 45%, 15%, 33% and 0% of samples of SS, MF, pcMZL and pcFCL, respectively. IgE‐t levels were also increased in SS, pcMZL and marginally MF. No correlation was found with patients’ history of atopic disorders. Staphylococcal superantigen‐specific IgE ≥ 0.35 kUa/L, most often against toxic shock syndrome toxin‐1, was detected in 40% of Sézary samples followed by MF (20%). In the absence of serologic atopy (negative Phadiatop test), IgE‐t levels for patients with SS and MF were not significantly higher than controls whereas the levels for pcMZL remained high. Furthermore, even with a negative Phadiatop test, IgE‐t values were higher in sera of patients with SSAg‐IgE ≥ 0.35 kUa/L vis‐à‐vis < 0.35 kUa/L across all diagnostic categories including controls albeit the difference was statistically significant only for SS. The presence of specific IgE antibodies ≥ 0.35 kUa/L, IgE‐t > 122 kU/L or eosinophils > 500/µL had no impact on survival of patients with SS. These results indicate that a pathogenic link may exist between an atopic diathesis and development of SS and possibly pcMZL

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Interleukin-15 Is an Autocrine/Paracrine Viability Factor for Cutaneous T-Cell Lymphoma Cells

Cited by 151 publications

References 29 publications

Establishment of a mouse xenograft model for mycosis fungoides

Establishment of a mouse xenograft model for mycosis fungoides

Cutaneous malignant lymphomas: Update 2006

Prevalence of atopy and staphylococcal superantigen‐specific immunoglobulin E (IgE) antibodies and total serum IgE in primary cutaneous T‐ and B‐cell lymphoma

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