Interleukin 16 is up-regulated in Crohn's disease and participates in TNBS colitis in mice

Keates, Andrew C.; Castagliuolo, Ignazio; Cruickshank, William W.; Qiu, Bosheng; Arseneau, Kristen O.; Brazer, William; Kelly, Ciarán P.

doi:10.1053/gast.2000.18164

Cited by 80 publications

(54 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…injection of ketamine hydrochloride (900 mg/g body weight) and xylazine hydrochloride (20 mg/g body weight) and colitis was induced by intrarectal instillation of 100 ml of a DNBS solution (40 mg/ml in 40% ethanol) as described. 52 At the time of DNBS administration, mice were randomly allocated to receive by oral gavage 10 8 CFU (total volume 100 ml) of either invasive E. coli BM2710/pGB2Oinv-hly or noninvasive BM2710pGB2 harboring one of the following plasmids: pC1, pC1OTGF-b1 or pC1OTGF-b1 IND . A group of mice received the intrarectal instillation of DNBS and received by oral gavage 100 ml BHI (nottreated control).…”

Section: In Vivo Studies and Tissue Analysesmentioning

confidence: 99%

“…The whole colon was removed, flat opened, rinsed in ice-cold PBS, and a macroscopic damage score (0-10) was assigned by two independent investigators, unaware of the treatment administered to each mouse, as an index of disease activity. 52 Each colon was then divided in two longitudinally: one part was fixed in 4% paraformaldehyde (PFA) for subsequent histological examination, 52 and the other was snap frozen in liquid nitrogen and stored at À801C to assess MPO, as a marker of leukocyte infiltration, or to extract total tissue RNAs. 53 …”

Section: In Vivo Studies and Tissue Analysesmentioning

confidence: 99%

“…52 Contaminating DNA was removed with RQ-1 RNase-free DNase (Promega) and random primed cDNAs were generated using MuLV reverse transcriptase (Applied Biosystems, Foster City, CA, USA). PCR was performed for mouse GAPDH (housekeeping Engineered E. coli delivers genes to the colonic mucosa I Castagliuolo et al control gene) and vector-specific TGF-b1 transcripts as summarized in Table 1.…”

Section: Rna Isolation and Quantitative Real-time Rt-pcr Analysismentioning

confidence: 99%

See 2 more Smart Citations

Engineered E. coli delivers therapeutic genes to the colonic mucosa

et al. 2005

View full text Add to dashboard Cite

Taking advantage of the proximity of bowel mucosa to luminal bacteria, we have attempetd to deliver a therapeutic gene to the colonic mucosa by oral administration of an invasive and non-pathogenic Escherichia coli. E. coli diamenopimelate (dap) auxotroph, harboring plasmid pGB2Oinv-hly, express the inv gene from Yersinia pseudotubercolosis that confers the ability to invade nonprofessional phagocytic cells and the hly gene from Listeria monocytogenes that allows expression of lystreriolysin O, a perforin cytolysin able to perfore phagosomal membranes. This bacterial vector invades and transfers functional DNA to epithelial cells in vitro. We have shown that this strain carrying a therapeutic gene (pC1OTGF-b1) can significantly reduce the severity of experimental colitis in mice. However, as a consequence of mucosal barrier disruption during colitis, vector-specific mRNA transcripts could be recovered from the colon and also from extra-colonic tissues. We therefore replaced the constitutive CMV promoter in pC1OTGF-b1 by the inflammation-inducible interleukin-8 promoter generating plasmid pC1OTGF-b1 IND . Plasmid-specific TGF-b1 mRNA transcripts were detectable in mouse CMT-93 epithelial cells incubated with E. coli BM2710/pGB2Oinv-hly carrying pC1OTGF-b1 IND following exposure to inflammatory cytokines. Furthermore, the transcripts were detectable only within inflamed tissues and the therapeutic effects were comparable to those in animals treated with E. coli BM2710/ pGB2Oinv-hly+pC1OTGF-b1. In summary, engineered enteric bacteria can efficiently deliver in vivo therapeutic genes to the intact intestinal mucosa and regulation expression of the therapeutic gene by an inflammation-inducible promoter prevents its dissemination during colitis.

show abstract

Section: In Vivo Studies and Tissue Analysesmentioning

confidence: 99%

Section: In Vivo Studies and Tissue Analysesmentioning

confidence: 99%

Section: Rna Isolation and Quantitative Real-time Rt-pcr Analysismentioning

confidence: 99%

See 1 more Smart Citation

Engineered E. coli delivers therapeutic genes to the colonic mucosa

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In mice, this model is widely used to study IBD because of clinical and histopathological findings resembling those seen in CD [49,50] . Using a wide range of TNBS concentrations, the authors observed a reduction of fish survival in a dose-dependent way with a recovery in survival rate when fishes were treated with vancomycin.…”

Section: Chemically Induced Adult Modelsmentioning

confidence: 99%

Inflammatory diseases modelling in zebrafish

Fénero

Flores

Câmara

2016

WJEM

View full text Add to dashboard Cite

The ingest of diets with high content of fats and carbohydrates, low or no physical exercise and a stressful routine are part of the everyday lifestyle of most people in the western world. These conditions are triggers for different diseases with complex interactions between the host genetics, the metabolism, the immune system and the microbiota, including inflammatory bowel diseases (IBD), obesity and diabetes. The incidence of these disorders is growing worldwide; therefore, new strategies for its study are needed. Nowadays, the majority of researches are in use of murine models for understand the genetics, physiopathology and interaction between cells and signaling pathways to find therapeutic solutions to these diseases. The zebrafish, a little tropical water fish, shares 70% of our genes and conserves anatomic and physiological characteristics, as well as metabolical pathways, with mammals, and is rising as a new complementary model for the study of metabolic and inflammatory diseases. Its high fecundity, fast development, transparency, versatility and low cost of maintenance makes the zebrafish an interesting option for new researches. In this review, we offer a discussion of the existing genetic and induced zebrafish models of two important Western diseases that have a strong inflammatory component, the IBD and the obesity. Core tip:The western lifestyle with a high fat and carbohydrates diet, lack of physical activity and stress, is a trigger for different diseases with complex interactions between the host genetics, the metabolism, the immune system and the microbiota, as the inflammatory bowel disease (IBD), obesity and diabetes. The zebrafish has 70% homology with our genes, shares anatomic and physiological characteristics with mammals, and emerges as a new model for the study of metabolic and inflammatory diseases. In this review, we examine the existing genetic and induced zebrafish models of two important Western diseases with strong inflammatory component, IBD and obesity.Morales Fénero CI, Colombo Flores AA, Câmara NOS. Inflammatory diseases modelling in zebrafish. World J Exp Med 2016; 6(1): 9-20 Available from:

show abstract

“…It has been demonstrated that IL-12p40 KO mice develop severe TNBS-induced colitis. Moreover, administration of IL-12p40 neutralizing antibody increases pathology in IL12p35 KO mice, which suggests that IL-12p40, in contrast Pathogenic factors Categories Factors (References) T cells Th1 [8] , CD8 + TCR Vβ14 + T cell [21] , CEACAM1 [27] Cytokines/chemokines IL-12 [8,30] , IFN-γ [8,34] , IL-18 [31,32,139] , IL-6 [73] , IL-16 [140] , IL-17 [38] , TNF-α [29,141] , MIP-α [142] , MIP-3α [143] Receptors CD40 [57,58] , CD44v7 [144] , FcεRI [145] , GITR [63,64] , Complement receptor 3 [146] Transcription factors NF-κB p65 [65,67,147] , RICK [69,70] , MAPK p38 [70] , Smad7 [72] , Smad3 [148] Adhesion molecules Integrinα1β1 [80] Enzymes Poly (ADP-ribose) synthetase [149,150] , Inducible nitric oxide synthase [151] , Angiotensinogen [152] , Vanin-1 [107] Hormones Leptin [113] , Ghrelin [153] , Adiponectin [112] Others Geneticfactors [11] , Glycolipid [154] Regulatory factors Categories Factors (References) T cells TCRγδ …”

Section: Pathogenic Factors Categories Factors (References) Chemokinementioning

confidence: 99%

Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease

Kawada

Arihiro²,

Mizoguchi³

2007

WJG

170

131

View full text Add to dashboard Cite

Inflammatory bowel disease (IBD), the most important being Crohn's disease and ulcerative colitis, results from chronic dysregulation of the mucosal immune system in the gastrointestinal tract. Although the pathogenesis of IBD remains unclear, it is widely accepted that genetic, environmental, and immunological factors are involved. Recent studies suggest that intestinal epithelial defenses are important to prevent inflammation by protecting against microbial pathogens and oxidative stresses. To investigate the etiology of IBD, animal models of experimental colitis have been developed and are frequently used to evaluate new anti-inflammatory treatments for IBD. Several models of experimental colitis that demonstrate various pathophysiological aspects of the human disease have been described. In this manuscript, we review the characteristic features of IBD through a discussion of the various chemically induced experimental models of colitis (e.g., dextran sodium sulfate-, 2,4,6-trinitrobenzene sulfonic acid-, oxazolone-, acetic acid-, and indomethacin-induced models). We also summarize some regulatory and pathogenic factors demonstrated by these models that can, hopefully, be exploited to develop future therapeutic strategies against IBD.

show abstract

Interleukin 16 is up-regulated in Crohn's disease and participates in TNBS colitis in mice

Cited by 80 publications

References 38 publications

Engineered E. coli delivers therapeutic genes to the colonic mucosa

Engineered E. coli delivers therapeutic genes to the colonic mucosa

Inflammatory diseases modelling in zebrafish

Insights from advances in research of chemically induced experimental models of human inflammatory bowel disease

Contact Info

Product

Resources

About