Interleukin-17 expression in the serum and exfoliated cervical cells of patients infected with high-risk oncogenic human papillomavirus

Bonin, Camila Mareti; Almeida-Lugo, Larissa Zatorre; Padovani, Cacilda Tezelli Junqueira; Pina, Ana Flávia Silva; Ferreira, Alda Maria Teixeira; Fernandes, Carlos Eurico dos Santos; Possati-Resende, Júlio César; Bovo, Adriane Cristina; Tozetti, Inês Aparecida

doi:10.1016/j.cyto.2019.04.008

Cited by 13 publications

(16 citation statements)

References 65 publications

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“…The samples used in this study were obtained from a previous study in which 410 women older than 18 years were subjected to identification and genotyping of HPV as well as the identification of the presence of co-infections [15]. The case group was composed of serum and exfoliated cervical cell (ECC) samples from 26 of 410 women positive for HR-HPV DNA selected using non-probability sampling for convenience.…”

Section: Study Populationmentioning

confidence: 99%

“…HPV genotype assays were performed via polymerase chain reaction using PGMY primers, followed by type-specific PCR (TS-PCR) and restriction fragment length polymorphism (RFLP) S1 and S2 Tables. Samples that were not genotyped through TS-PCR and RFLP were sequenced using an ABI-Prism 3500 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) [15].…”

Section: Study Populationmentioning

confidence: 99%

“…None of the patients included in the present study had undergone a hysterectomy or had exposure to immunosuppressive drugs, and none were pregnant or in their menstrual period. These patients were also negative for Chlamydia trachomatis and Trichomonas vaginalis DNA, anti-human immunodeficiency virus (HIV) and anti-Treponema pallidum, and circulating hepatitis B virus surface antigen (HBsAg) [15] to exclude any systemic and local co-infections in the uterine cervix that could influence the cytokine levels. In addition to serum and ECC samples, we also used samples of cervical biopsies from women positive for HR-HPV with high-grade squamous intraepithelial lesion (HSIL), low-grade squamous intraepithelial lesion (LSIL), and cervical cancer (CC) lesions from previous studies [15,16].…”

Section: Study Populationmentioning

confidence: 99%

“…In this study, samples of ECC and serum were used to measure the expression levels of cytokines IFN-γ, TNF-α, IL-10, IL-6, IL-4, and IL-2, and samples of uterine cervix biopsies were used to identify the presence of IL-6 through immunohistochemistry. All samples used in this study were obtained from previous studies [15,16].…”

Section: Acquisition Of Samplesmentioning

confidence: 99%

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IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus

et al. 2021

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Persistent infection by high-risk human papillomavirus (HR-HPV) is the main cause of cervical cancer and its precursor lesions. While some cytokines help immune cells in virus clearance, others contribute to the persistence of infection and neoplastic progression. Here, the levels of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-10, IL-6, IL-4, and IL-2 were quantified in the serum and exfoliated cervical cells (ECCs) of patients with HR-HPV, and the presence of IL-6+ cells was investigated in uterine cervix biopsies. Cytokine levels in the serum and ECCs of 26 HR-HPV DNA-positive patients and 18 HPV DNA-negative patients were measured using flow cytometry. Fifteen uterine cervix biopsy samples embedded in paraffin were subjected to immunohistochemical analysis for the detection of IL-6+ cells. HR-HPV-positive patients showed increased IL-6 and IL-10 in the ECCs and serum, respectively. Compared with HPV DNA-positive patients, HPV DNA-negative patients had higher levels of IL-6 in ECCs. Patients with multiple infections of HPV had higher levels of IL-6 in their ECCs than those with a single infection. Immunostaining of uterine cervix biopsy samples revealed no differences in IL-6 expression between the different classes of histopathological lesions. However, differences were observed in the expression levels of IL-6 and IL-10 at the systemic and local levels in HR-HPV-positive patients without cervical lesions. Considering the functional characteristics of these cytokines, it can be inferred that such patients are prone to persistent HPV infection.

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Section: Study Populationmentioning

confidence: 99%

Section: Study Populationmentioning

confidence: 99%

Section: Study Populationmentioning

confidence: 99%

Section: Acquisition Of Samplesmentioning

confidence: 99%

See 2 more Smart Citations

IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus

et al. 2021

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“…This long-term viral secretion indicates ZIKV may establish persistent infections to develop in the urogenital tract. It is hypothesized that other viruses, such as Human Immunodeficiency Virus (HIV), Hepatitis C virus (HCV), cytomegalovirus, and human papillomavirus, are able to develop persistent infections by altering specific cytokine responses responsible for increasing host cell susceptibility or augmenting viral replication [11][12][13][14]. However, little is known about which persistence mechanisms mediate longterm ZIKV sexual transmission.…”

Section: Introductionmentioning

confidence: 99%

IP-10 and CXCR3 signaling inhibit Zika virus replication in human prostate cells

et al. 2020

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Our previous studies have shown that Zika virus (ZIKV) replicates in human prostate cells, suggesting that the prostate may serve as a long-term reservoir for virus transmission. Here, we demonstrated that the innate immune responses generated to three distinct ZIKV strains (all isolated from human serum) were significantly different and dependent on their passage history (in mosquito, monkey, or human cells). In addition, some of these phenotypic differences were reduced by a single additional cell culture passage, suggesting that viruses that have been passaged more than 3 times from the patient sample will no longer reflect natural phenotypes. Two of the ZIKV strains analyzed induced high levels of the IP-10 chemokine and IFNγ in human prostate epithelial and stromal mesenchymal stem cells. To further understand the importance of these innate responses on ZIKV replication, we measured the effects of IP-10 and its downstream receptor, CXCR3, on RNA and virus production in prostate cells. Treatment with IP-10, CXCR3 agonist, or CXCR3 antagonist significantly altered ZIKV viral gene expression, depending on their passage in cells of relevant hosts (mosquito or human). We detected differences in gene expression of two primary CXCR3 isoforms (CXCR3-A and CXCR3-B) on the two cell types, possibly explaining differences in viral output. Lastly, we examined the effects of IP-10, agonist, or antagonist on cell death and proliferation under physiologically relevant infection rates, and detected no significant differences. Although we did not measure protein expression directly, our results indicate that CXCR3 signaling may be a target for therapeutics, to ultimately stop sexual transmission of this virus.

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Interleukin‐17—A multifaceted cytokine in viral infections

Sahu

Biswas

Prajapati

et al. 2021

Journal Cellular Physiology

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Viral infections are a major threat to the human population due to the lack of selective therapeutic measures. The morbidity and mortality reported worldwide are very alarming against viral pathogens. The proinflammatory environment is required for viral inhibition by initiating the host immune response. The host immune response fights these pathogens by secreting different cytokines. Interleukin‐17 (IL‐17) a proinflammatory cytokine mainly produced by T helper type 17 cells, plays a vital role in the regulation of host immune response against various pathogens, including viruses. However, dysregulated production of IL‐17 induces chronic inflammation, autoimmune disorders, and may lead to cancer. Recent studies suggest that IL‐17 is not only involved in the antiviral immune response but also promotes virus‐mediated illnesses. In this review, we discuss the protective and pathogenic role of IL‐17 against various viral infections. A detailed understanding of IL‐17 during viral infections could contribute to improve therapeutic measures and enable the development of an efficient and safe IL‐17 based immunotherapy.

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Interleukin-17 expression in the serum and exfoliated cervical cells of patients infected with high-risk oncogenic human papillomavirus

Cited by 13 publications

References 65 publications

IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus

IL-6 and IL-10 in the serum and exfoliated cervical cells of patients infected with high-risk human papillomavirus

IP-10 and CXCR3 signaling inhibit Zika virus replication in human prostate cells

Interleukin‐17—A multifaceted cytokine in viral infections

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