Interleukin-18 is expressed in rat testis and may promote germ cell growth

Strand, Mona-Lisa; Wahlgren, Aida; Svechnikov, Konstantin; Zetterström, Cecilia; Setchell, B. P.; Söder, Olle

doi:10.1016/j.mce.2005.05.011

Cited by 21 publications

(35 citation statements)

References 43 publications

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“…The role of this cytokine in human testicular physiology is unknown but Soder's group results suggest its host-protecting and growth-promoting function in the rat testis. 35 The origin of IL-18 in rat seminiferous tubules was rather restricted to spermatocytes and spermatids. 35 This phenomenon seems to be observed (at least at mRNA level) also in human testis.…”

Section: Interleukin-1 Superfamily Genes Expression N Rozwadowska Et Almentioning

confidence: 99%

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Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

et al. 2007

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Interleukin-1 (IL-1) is a pleiotropic cytokine that may play a role in contributing to the specific immune environment of mammalian testis and in regulating cell differentiation. We have determined the transcription activity of the IL-1 gene family (using real-time polymerase chain reaction (PCR)) in two main functional testicular compartments (interstitial and intratubular ones), and in tissue homogenates obtained from patients with fertility disorders (spermatogenic arrest and testicular tumors). We observed the prominent expression of gene coding for IL-1 receptor antagonist (IL-1RA) in a purified fraction of gametogenic cells (normal gonad). Caspase-1 (ICE -IL-1b-converting enzyme) was highly expressed (on mRNA level) in interstitial compartments as well in testicular tumors (immune enhancement?). In addition we found, that the activity of IL-1RA gene decreased along spermatogenic alteration in an inversely related manner with IL-1a (from normal gonad through spermatogenic arrest to Sertoli cell only syndrome). Therefore, the quotient value of IL-1a/IL-1RA could potentially serve as the diagnostic molecular probe for spermatogenesis assessment. The precise level of mRNA for IL-1-IL-18 cytokines and their receptors, and specifically of the receptor antagonist in immune privileged gonad, could be one of the main factors responsible for maintaining testicular homeostasis, thus enabling generation of the mature spermatozoa.

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Section: Interleukin-1 Superfamily Genes Expression N Rozwadowska Et Almentioning

confidence: 99%

“…35 The origin of IL-18 in rat seminiferous tubules was rather restricted to spermatocytes and spermatids. 35 This phenomenon seems to be observed (at least at mRNA level) also in human testis.…”

Section: Interleukin-1 Superfamily Genes Expression N Rozwadowska Et Almentioning

confidence: 99%

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

et al. 2007

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“…IL18 may also regulate spermatogenesis (Strand et al 2005, Abu Elhija et al 2008a, Komsky et al 2011. Moreover, Strand et al (2005) suggested that endogenous IL18 derived from testicular cells may mitigate the harmful effects of infection/inflammation on spermatogenesis. Therefore, we speculate that testicular function during the recovery phase of endotoxemia in our mouse model was not regulated appropriately in the absence of IL18 and as a result germ cell apoptosis increased.…”

Section: Discussionmentioning

confidence: 99%

“…In the intestine, monocyte-derived IL18 induced epithelial reconstitution during acute inflammation, but during chronic inflammation, lymphocyte-derived IL18 induced epithelial injury. In the testes, endogenous IL18 is continuously produced by the testicular cells (Strand et al 2005, Abu Elhija et al 2008b, Komsky et al 2011. After infection, macrophages, dendritic cells and lymphocytes migrate to the testes (Jacobo et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Under physiologic conditions, IL18 regulates testicular function, development, and spermatogenesis (Strand et al 2005, Abu Elhija et al 2008b, Komsky et al 2011. IL18 is a mediator of apoptosis through both the death receptor (extrinsic) and the mitochondrial (intrinsic) pathways (Mariño & Cardier 2003, Chandrasekar et al 2004, Akhtar et al 2011, Zhang et al 2011.…”

Section: Introductionmentioning

confidence: 99%

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Endogenous interleukin 18 regulates testicular germ cell apoptosis during endotoxemia

Inoue¹,

Ishikawa²,

Kamoshida³

et al. 2015

Reproduction

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Orchitis (testicular swelling) often occurs during systemic inflammatory conditions, such as sepsis. Interleukin 18 (IL18) is a proinflammatory cytokine and is an apoptotic mediator during endotoxemia, but the role of IL18 in response to inflammation in the testes was unclear. WT and IL18 knockout (KO) mice were injected lipopolysaccharide (LPS) to induce endotoxemia and examined 12 and 48 h after LPS administration to model the acute and recovery phases of endotoxemia. Caspase activation was assessed using immunohistochemistry. Protein and mRNA expression were examined by western blot and quantitative real-time RT-PCR respectively. During the acute phase of endotoxemia, apoptosis (as indicated by caspase-3 cleavage) was increased in WT mice but not in IL18 KO mice. The death receptor-mediated and mitochondrial-mediated apoptotic pathways were both activated in the WT mice but not in the KO mice. During the recovery phase of endotoxemia, apoptosis was observed in the IL18 KO mice but not in the WT mice. Activation of the death-receptor mediated apoptotic pathway could be seen in the IL18 KO mice but not the WT mice. These results suggested that endogenous IL18 induces germ cell apoptosis via death receptor mediated-and mitochondrial-mediated pathways during the acute phase of endotoxemia and suppresses germ cell apoptosis via death-receptor mediated pathways during recovery from endotoxemia. Taken together, IL18 could be a new therapeutic target to prevent orchitis during endotoxemia.Reproduction (2015) 150 105-114

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Congenital Diseases and the Environment

Nicolopoulou-Stamati¹,

Hens²,

Howard³

2007

Environmental Science and Technology Library

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It is the policy of AREHNA, EU-SANCO project, to encourage the full spectrum of opinions to be represented at its meetings. Therefore it should not be assumed that the publication of a paper in this volume implies that the Editorial Board is fully in agreement with the contents, though we ensure that contributions are factually correct. Where, in our opinion, there is scope for ambiguity we have added notes to the text, where appropriate.

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Interleukin-18 is expressed in rat testis and may promote germ cell growth

Cited by 21 publications

References 43 publications

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

Interleukin-1 superfamily genes expression in normal or impaired human spermatogenesis

Endogenous interleukin 18 regulates testicular germ cell apoptosis during endotoxemia

Congenital Diseases and the Environment

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