Interleukin 1β and stromelysin (MMP3) activity of synovial fluid as possible markers of osteoarthritis in the temporomandibular joint

Kubota, Eiro; Imamura, Hideo; Kubota, Takao; Shibata, Takanori; Murakami, Ken-Ichiro

doi:10.1016/s0278-2391(97)90438-9

Cited by 186 publications

(138 citation statements)

References 20 publications

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“…Interleukin (IL)-1 is elevated in the synovial fluid of patients with rheumatoid arthritis 6) , where it amplifies and perpetuates inflammation 7) and induces joint destruction 8) . In several studies, an elevated concentration of IL-1β was found in synovial fluid samples from TMJ patients with osteoarthritis and was related to the radiographic signs of TMJ destruction 9,10,11) . It has been demonstrated that IL-1β may stimulate cartilage matrix turnover by increasing MMP levels 12) .…”

Section: Introductionmentioning

confidence: 99%

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Nakayama

Aida

Watanabe

et al. 2012

J. Hard Tissue Biology.

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Section: Introductionmentioning

confidence: 99%

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Nakayama

Aida

Watanabe

et al. 2012

J. Hard Tissue Biology.

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“…degenerated OA cartilage (Saha et al 1999) as well as in the synovial membranes and synovial fluids of OA patients (Smith et al 1997;Kubota et al 1997). At the cellular level, chondrocytes respond to this cytokine via a cascade of events leading to loss of balance between cartilage anabolism and catabolism, associated with phenotypic modulation and activation of the normally quiescent chondrocytes (Goldring and Marcu 2009;Goldring et al 2008).…”

mentioning

confidence: 99%

Caesalpinia sappan extract inhibits IL1β-mediated overexpression of matrix metalloproteinases in human chondrocytes

Toegel

Otero

et al. 2011

Genes Nutr

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Exacerbated production of matrix metalloproteinases (MMPs) is a key event in the progression of osteoarthritis (OA) and represents a promising target for the management of OA with nutraceuticals. In this study, we sought to determine the MMP-inhibitory activity of an ethanolic Caesalpinia sappan extract (CSE) in human OA chondrocytes. Thus, human articular chondrocytes isolated from OA cartilage and SW1353 chondrocytes were stimulated with Interleukin-1beta (IL1b), without or with pretreatment with CSE. Following viability assays, the production of MMP-2 and MMP-13 was assessed using ELISA, whereas mRNA levels of MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-13 and TIMP-1, TIMP-2, TIMP-3 were quantified using RT-qPCR assays. Chondrocytes were co-transfected with a MMP-13 luciferase reporter construct and NF-kB p50 and p65 expression vectors in the presence or absence of CSE. In addition, the direct effect of CSE on the proteolytic activities of MMP-2 was evaluated using gelatin zymography. We found that CSE significantly suppressed IL1b-mediated upregulation of MMP-13 mRNA and protein levels via abrogation of the NF-kB(p65/p50)-driven MMP-13 promoter activation. We further observed that the levels of IL1b-induced MMP-1, MMP-3, MMP-7, and MMP-9 mRNA, but not TIMP mRNA levels, were down-regulated in chondrocytes in response to CSE. Zymographic results suggested that CSE did not directly interfere with the proteolytic activity of MMP-2. In summary, this study provides evidence for the MMP-inhibitory potential of CSE or CSE-derived compounds in human OA chondrocytes. The data indicate that the mechanism of this inhibition might, at least in part, involve targeting of NF-kB-mediated promoter activation.

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“…The cytokines are produced by chondrocytes, synovial macrophages, or synovial fibroblasts [22][23][24][25][26]. The inflammatory process activate enzymes degrading synovium and articular cartilage [27].…”

Section: Pathology Of Temporomandibular Jointmentioning

confidence: 99%

“…It is elucidated, that excess amount of the inflammatory mediators in the synovial fluid of TMJ in patients with TMD is important, and also their characteristics, balance and theirs receptors [22][23][24][25][26]30]. There are the mutually protected and regulated mechanisms of inflammation.…”

Section: Inflammatory Mediators In the Temporomandibular Joint Synovimentioning

confidence: 99%

“…There was no correlation between the amount of any cytokines and clinical symptoms. Other authors also suggest that IL-1 and stromelysin (MMP3) can be the markers of early bony deterioration, which is not detected by radiograph imaging [24]. It was revealed that in bony degrading stage of internal derangements, the IL-1 is produced by macrophages from synovium but also by chondrocytes or fibroblasts from articular cartilage, that suggest the possibility of detection the early OA changes.…”

Section: Inflammatory Mediators In the Temporomandibular Joint Synovimentioning

confidence: 99%

See 1 more Smart Citation

Inflammatory markers in temporomandibular joint disorders

Kostrzewa-Janicka¹,

Jurkowski²,

Nędzi-Góra³

et al. 2012

cejoi

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Interleukin 1β and stromelysin (MMP3) activity of synovial fluid as possible markers of osteoarthritis in the temporomandibular joint

Cited by 186 publications

References 20 publications

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Influence of Compressive Force and IL-1^|^beta; on Metabolism of the Extracellular Matrix in Human Chondrocytes

Caesalpinia sappan extract inhibits IL1β-mediated overexpression of matrix metalloproteinases in human chondrocytes

Inflammatory markers in temporomandibular joint disorders

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