Interleukin 1β (IL-1β) release from fresh and cultured colonic mucosa in patients with ulcerative colitis (UC)

Gionchetti, Paolo; Campieri, Massimo; Belluzzi, Andrea; Tampieri, M.; Bertinelli, E.; Brignola, C.; Ferretti, Maurizio; Boni, P; Iannone, Primiano; Miglioli, M.; Leggett, Barbara A.

doi:10.1007/bf01991024

Cited by 11 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, leukocytes from the intestinal draining lymph nodes secrete multiple T H 1-, T H 2-, and T H 17-type cytokines in a complex pattern as in IBD. 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 Loss of T and B lymphocytes did not completely ameliorate colitis but altered the colonic distribution of inflammation in young mice and prevented progression of colonic inflammation. Taken together with the rapid activation and severe colitis induced following T-cell transfer into Winnie mice lacking lymphocytes, these data show that this primary epithelial defect leads to inflammation driven by both innate and adaptive immunity.…”

Section: Discussionmentioning

confidence: 95%

An intestinal epithelial defect conferring ER stress results in inflammation involving both innate and adaptive immunity

et al. 2011

View full text Add to dashboard Cite

We recently characterized Winnie mice carrying a missense mutation in Muc2, leading to severe endoplasmic reticulum stress in intestinal goblet cells and spontaneous colitis. In this study, we characterized the immune responses due to this intestinal epithelial dysfunction. In Winnie, there was a fourfold increase in activated dendritic cells (DCs; CD11c+ major histocompatibility complex (MHC) class IIhi) in the colonic lamina propria accompanied by decreased colonic secretion of an inhibitor of DC activation, thymic stromal lymphopoietin (TSLP). Winnie also displayed a significant increase in mRNA expression of the mucosal TH17 signature genes Il17a, IL17f, Tgfb, and Ccr6, particularly in the distal colon. Winnie mesenteric lymph node leukocytes secreted multiple TH1, TH2, and TH17 cytokines on activation, with a large increase in interleukin-17A (IL-17A) progressively with age. A major source of mucosal IL-17A in Winnie was CD4+ T lymphocytes. Loss of T and B lymphocytes in Rag1-/- × Winnie (RaW) crosses did not prevent spontaneous inflammation but did prevent progression with age in the colon but not the cecum. Adoptive transfer of naive T cells into RaW mice caused more rapid and severe colitis than in Rag1-/-, indicating that the epithelial defect results in an intestinal microenvironment conducive to T-cell activation. Thus, the Winnie primary epithelial defect results in complex multicytokine-mediated colitis involving both innate and adaptive immune components with a prominent IL-23/TH17 response, similar to that of human ulcerative colitis.

show abstract

Section: Discussionmentioning

confidence: 95%

An intestinal epithelial defect conferring ER stress results in inflammation involving both innate and adaptive immunity

et al. 2011

View full text Add to dashboard Cite

show abstract

“…1,2 Inflammatory cytokines and free radicals present in the intestinal mucosa of patients with UC are thought to play an integral part in the inflammatory process. [3][4][5][6][7][8][9][10][11][12][13][14][15] Numerous experimental models of UC are initiated by acute chemical insults to the intestinal mucosa. Although these models reproduce some histologic features of UC, they may not reflect the progression of the disease or accurately reproduce chronic inflammatory changes and motor function disturbances in the muscularis propria.…”

mentioning

confidence: 99%

Abnormal Motility in Patients With Ulcerative Colitis

Vrees

Pricolo²,

Potenti³

et al. 2002

Arch Surg

View full text Add to dashboard Cite

show abstract

“…IL-12, which is a proinflammatory cytokine, plays an important role in the pathogenesis of UC [ 23 ]. IL-1 β is a key mediator of the inflammatory and immunological response, and several biological properties may be of relevance in the pathogenesis of UC [ 24 ]. Meanwhile, IL-1 β promotes the expressions of other inflammatory cytokines [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Effects of Essential Oil Obtained from the Crude and Bran-Processed Atractylodes lancea on Lipopolysaccharide-Induced Inflammatory Injury of Human Colonic Epithelial Cells by Downregulating the IKK/NF-κB Signaling Pathway

Han

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Background. Atractylodes lancea (AL) has been used in traditional Chinese medicine for the treatment of various diseases including digestive disorders. Ulcerative colitis (UC) is a common digestive system disease with a low cure rate and easy recurrence. However, it is still not clear whether AL is suitable for UC treatment. Currently, stir-baking with wheat bran is most commonly used to process AL. Here, we aimed to address the effects of the crude and bran-processed AL on UC in vitro and uncover the underlying mechanism based on regulating the IKK/NF-kappa B signaling pathway. Methods. Human colonic epithelial cells (HCoEpiC) were treated with lipopolysaccharide (LPS) to mimic the inflammatory injury of UC in vitro. The essential oil from crude and bran-processed AL was used to treat LPS-induced HcoEpiC cells. The cell viability was detected by an MTT assay. The levels of IL-4, IL-6, IL-8, IL-12, IL-1-β, TNF-α, and NO were determined by ELISA, and the mRNA expressions of IKK-α, NF-κB, IL-4, IL-6, IL-8, and TNF-α were determined by RT-PCR. Meanwhile, the expressions of IKK-α, p-IKK-α, p-IKK-β, NF-κB, IL-6, and IL-8 proteins were determined by Western blot. Results. The essential oil of AL, whether it was from crude or bran-processed AL, could significantly increase the viability of LPS-induced HCoEpiC cells. The treatment of AL essential oil also notably inhibited the productions of IL-6, IL-8, IL-12, IL-1-β, TNF-α, NO, p-IKK-α, p-IKK-β, and NF-κB and downregulated the mRNA expressions of NF-κB, IL-6, IL-8, and TNF-α. Meanwhile, IL-4 protein and mRNA expression were significantly stimulated by AL essential oil. Moreover, the essential oil from bran-processed AL was more effective than that from crude AL. Conclusion. Both kinds of AL essential oil had the anti-inflammatory effect on LPS-induced HCoEpiC, and the essential oil from bran-processed AL was more effective. The mechanism could be through the IKK/NF-κB signaling pathway in vitro.

show abstract

Interleukin 1β (IL-1β) release from fresh and cultured colonic mucosa in patients with ulcerative colitis (UC)

Cited by 11 publications

References 10 publications

An intestinal epithelial defect conferring ER stress results in inflammation involving both innate and adaptive immunity

An intestinal epithelial defect conferring ER stress results in inflammation involving both innate and adaptive immunity

Abnormal Motility in Patients With Ulcerative Colitis

Comparison of the Effects of Essential Oil Obtained from the Crude and Bran-Processed Atractylodes lancea on Lipopolysaccharide-Induced Inflammatory Injury of Human Colonic Epithelial Cells by Downregulating the IKK/NF-κB Signaling Pathway

Contact Info

Product

Resources

About