Interleukin-2 (IL-2) Interacts With IL-2 Receptor Beta (IL-2Rβ): Its Potential to Enhance the Proliferation of CD4+ T Lymphocytes in Flounder (Paralichthys olivaceus)

Zhou, Xiujuan; Xing, Jing; Tang, Xiaoqian; Sheng, Xiuzhen; Chi, Heng; Zhan, Wenbin

doi:10.3389/fimmu.2020.531785

Cited by 26 publications

(11 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the same time, T-bet was mainly expressed in CD4 + helper T cells, which explains the proliferation of CD4-1 + and CD4-2 + T cells in flounder after IFN-γ stimulation. In our previous study, we found that IL-2 was able to induce proliferation of CD4 + T cells through specific binding to its receptor IL-2R, and T-bet gene expression was found to be upregulated after stimulation with IL-2 ( 32 ), which is consistent with the results of the present study, where IL-2 was able to induce an increase in T-bet + cells and CD4 + T cell subpopulations. In the previously study, CD4-2 + T cells, rather than CD4-1 + T cells have a main role in Th1 immune responses, collaborating with CD8α and CD8β positive cells in flounder ( 46 ).…”

Section: Discussionsupporting

confidence: 92%

“…Lymphocytes were isolated from 18 flounder head kidney with aseptic technique in each group, and 72 (a half of fish used for flow cytometry, and a half of fish used for cell sorting) flounder were totally used in this experiment. Then, the cells were transferred to 24-well culture plates (800 μl/well) to ensure 5 × 10 6 cells per well, and cultured in L-15 medium with 10% fetal calf serum and penicillin/streptomycin supplemented with IFN-γ, IL-2, and IL-6 (100 ng/well) ( 32 ). PBS was added to the cells as control.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus)

et al. 2021

Self Cite

View full text Add to dashboard Cite

T-bet, a T-box family member, is a transcription factor essential for the differentiation of naive CD4+ T cells into Th1 cells that are involved in both innate and adaptive immune responses. In this study, the transcription factor T-bet of flounder (Paralichthys olivaceus) was cloned and characterized, and its expression profile after infection was analyzed. T-bet+ cells were identified in flounder, and the expression and localization of T-bet in T lymphocyte subsets and B lymphocytes were investigated. Finally, the proliferation of T-bet+ cells, T lymphocyte subsets, and B lymphocytes were studied after stimulation with IFN-γ, IL-2, and IL-6, respectively, and the variations of some transcription factors and cytokines in CD4+ T lymphocyte subsets were detected. The results showed that T-bet in flounder consists of 619 aa with a conserved T-box DNA binding domain. T-bet was abundantly expressed in the spleen, head kidney, and heart, and it was significantly upregulated after infection with Vibrio anguillarum, Edwardsiella tarda, and Hirame rhabdovirus, especially in the group of Edwardsiella tarda. A polyclonal antibody against recombinant protein of T-bet was prepared, which specifically recognized the natural T-bet molecule in flounder. T-bet+ cells were found to be distributed in the lymphocytes of peripheral blood, spleen, and head kidney, with the highest proportion in spleen, and the positive signals of T-bet occurred in the cell nucleus. T-bet was also detected in the sorted CD4-1+, CD4-2+, CD8+ T lymphocytes, and IgM+ B lymphocytes. In addition, T-bet+ cells, coordinated with CD4-1+ and CD4-2+ T lymphocytes, were proliferated after stimulation with IFN-γ, IL-2, and IL-6. Especially in sorted CD4-1+ and CD4-2+ T lymphocytes, IFN-γ and IL-2 were able to upregulate the expression of T-bet, forming a positive feedback loop in Th1-type cytokine secretion. These results suggest that T-bet may act as a master transcription factor regulating flounder CD4+ T lymphocytes involved in a Th1-type immune response.

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus)

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Then, cells were washed three times in L-15 medium with 10% FBS and adjusted to 5 × 10 6 cells/ml and placed into a 12-well culture plate (2 ml/well) cultured in L-15 medium with 10% FBS. Stimulated cells were treated with 5 μg/ml PHA (Sigma) or 5 μg/ml PHA + 0.5 μg/ml anti-CD28 Abs ( 40 ). Unstimulated cells were treated with an equal amount of sterile PBS medium as negative controls.…”

Section: Methodsmentioning

confidence: 99%

“…Mouse polyclonal antibodies against flounder IL-2 (diluted at 1:500) and monoclonal antibodies against flounder IgM (diluted at 1:1,000), CD4-1 (diluted at 1:1,000), and CD4-2 (diluted at 1:1,000) were previously produced in our laboratory (38)(39)(40). The secondary antibodies included DyLight 488 (Abbkine; diluted at 1:1,000) rabbit or mouse IgG, DyLight 649 (Abbkine; diluted at 1:1,000) rabbit or mouse IgG, alkaline phosphatase (AP)-conjugated goat anti-rabbit IgG (H + L) (Abbkine; diluted at 1:5,000), and HRP-conjugated goat anti-mouse IgG (Abbkine; diluted at 1:5,000).…”

Section: Animals Cells and Antibodiesmentioning

confidence: 99%

The Expression of CD28 and Its Synergism on the Immune Response of Flounder (Paralichthys olivaceus) to Thymus-Dependent Antigen

et al. 2021

Self Cite

View full text Add to dashboard Cite

CD28 is well known as a critical T-cell costimulatory receptor involved in T cell activation by binding to its ligands. In this study, CD28 was cloned, and its expression profiles were characterized in flounder (Paralichthys olivaceus); variations of CD28+ cells after being stimulated with different types of antigens and the function of the CD28 costimulatory pathway on T-cell activation were investigated in vitro. fCD28 consists of four exons and three introns, and the full-length cDNA of fCD28 was 675-bp encoded 224 amino acids. The conserved motif (121TFPPPF126) binding to the CD80/86 ligand exists in the Ig-superfamily homology domain. The high expression of fCD28 is in gills, PBLs, head kidney, and spleen. CD28+ cells were co-localized with CD4+ T lymphocytes but not on IgM+ B lymphocyte cells. Moreover, the expression of CD28 was significantly varied in flounder after being stimulated by keyhole limpet hemocyanin (KLH) at both the transcriptional and cellular levels, while no significant differences were observed between lipopolysaccharide (LPS) stimulation and the control group. Notably, treatment of PBLs cultured in vitro with CD28 molecule-specific antibody (anti-CD28 Abs) and PHA produced more cell colonies and stimulated the proliferation of cultured leukocytes compared to PHA stimulation alone and the control group, and a higher level of IL-2 was detected in the culture medium. Meanwhile, anti-CD28 Abs increased the percent of CD28+ cells (10.41 ± 1.35%), CD4+ T lymphocytes (18.32 ± 2.15%), and CD28+/CD4+ double-positive cells (6.24 ± 1.52%). This effect also resulted in significant variations in the genes of cell membrane-bound molecules, cytokines, and related signaling pathways in cultured leukocytes, with significant changes in the genes of interleukin-2 (IL-2) and nuclear factor of activated T cells (NFAT) in the early stages of culture, and the expression of other molecules increased over time. These results proved the localization of the CD28 molecule on T lymphocytes in flounder, and anti-CD28 may act as the B7 ligand involved in T cell activation after antigen stimulation. These data provide a basis for a more in-depth study of the mechanism of the CD28 costimulatory pathway in T cell activation.

show abstract

“…Currently, the term "cytokine" encompasses many types of proteins, including interleukins, chemokines, and the tumor necrosis factor (TNF) family. Interleukins mediate interactions between immune cells and are able to promote cell proliferation, differentiation, and functional activation [8][9][10][11]. Chemokines induce cell migration and activation by binding to specific G-protein-coupled cell surface receptors on target cells, such as neutrophils, monocytes, and lymphocytes, which play a pivotal role in host immune defense [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Cytokines Induced by Edwardsiella tarda: Profile and Role in Antibacterial Immunity

Sun

Jiang

et al. 2021

Biomolecules

View full text Add to dashboard Cite

Edwardsiella tarda is a Gram-negative bacterial pathogen with a broad range of hosts, including fish and mammals. In the present study, we used an advanced antibody array technology to identify the expression pattern of cytokines induced by E. tarda in a mouse infection model. In total, 31 and 24 differentially expressed cytokines (DECs) were identified in the plasma at 6 h and 24 h post-infection (hpi), respectively. The DECs were markedly enriched in the Gene Ontology (GO) terms associated with cell migration and response to chemokine and in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways associated with immunity, diseases, and infection. Ten key DECs, including IL6 and TNF-α, were found to form extensive protein-protein interaction networks. IL6 was demonstrated to inhibit E. tarda infection and be required for E. tarda-induced inflammatory response. TNF-α also exerted an inhibitory effect on E. tarda infection, and knockdown of fish (Japanese flounder) TNF-α promoted E. tarda invasion in host cells. Together, the results of this study revealed a comprehensive profile of cytokines induced by E. tarda, thus adding new insights into the role of cytokine-associated immunity against bacterial infection and also providing the potential plasma biomarkers of E. tarda infection for future studies.

show abstract

Interleukin-2 (IL-2) Interacts With IL-2 Receptor Beta (IL-2Rβ): Its Potential to Enhance the Proliferation of CD4+ T Lymphocytes in Flounder (Paralichthys olivaceus)

Abstract: Interleukin-2 (IL-2) Interacts With IL-2 Receptor Beta (IL-2Rβ): Its Potential to Enhance the Proliferation of CD4+ T Lymphocytes in Flounder (Paralichthys olivaceus).

Cited by 26 publications

References 46 publications

Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus)

Identification and Characterization of a Master Transcription Factor of Th1 Cells, T-bet, Within Flounder (Paralichthys olivaceus)

The Expression of CD28 and Its Synergism on the Immune Response of Flounder (Paralichthys olivaceus) to Thymus-Dependent Antigen

Cytokines Induced by Edwardsiella tarda: Profile and Role in Antibacterial Immunity

Contact Info

Product

Resources

About