Interleukin-23 Facilitates Thyroid Cancer Cell Migration and Invasion by Inhibiting SOCS4 Expression via MicroRNA-25

Mei, Zhidan; Chen, Shiming; Chen, Chen; Xiao, Bokui; Li, Fen; Wang, Yongping; Tao, Zezhang

doi:10.1371/journal.pone.0139456

Cited by 31 publications

(29 citation statements)

References 33 publications

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“…These microRNAs induced ESCC cell migration and invasion through NRIP1. While a number of reports have identified miRNAs' role in tumor proliferation, apoptosis, differentiation, invasion and metastasis [24][25][26], there are few reports of the function of miR-548 and miR-576. To our knowledge, there are two applicable studies of miR-548 and two studies that report the function of miR-576.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation

Zhao

et al. 2018

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Nuclear receptor interacting protein (NRIP1), also known as RIP140, is a transcriptional co-regulator required for the maintenance of energy homeostasis and ovulation. Although several studies have identified roles for NRIP1 in various cell processes, the biological functions of NRIP1 in esophageal squamous cell carcinoma (ESCC) remain unknown. Herein, we demonstrate that NRIP1 inhibits the migration and invasion of ESCC cells. Further mechanistic studies revealed that NRIP1 is directly targeted by miR-548-3p and miR-576-5p. We then identified that miR-548-3p and miR-576-5p regulate the migration and invasion of ESCC cells by inhibiting NRIP1 expression. Interestingly, the expression of miR-548-3p and miR-576-5p in ESCC cell lines and ESCC tissues is up-regulated and NRIP1 is down-regulated relative to controls. A statistically significant inverse association was found between the expression levels of miR-548-3p/miR-576-5p and NRIP1. These combined results reveal novel functions for miR-548-3p, miR-576-5p, and NRIP1 in regulating ESCC cell migration and invasion which are important functions for the metastatic process in esophageal cancer.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation

Zhao

et al. 2018

neo

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“…The data does however provide novel evidence that SOCS-4 plays an active role in the regulation of migration in these two cell types. A study on a thyroid cancer cell line has suggested that SOCS-4 has an inhibitory effect on migration and that inhibition of SOCS-4 by miR-25 could promote thyroid cancer cell migration 30 . In the current study, particularly in terms of HaCaT keratinocytes, suppression of SOCS−4 inhibited migration, indicating a pro-migratory role for SOCS-4 in this cell line.…”

Section: Discussionmentioning

confidence: 99%

In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing

Feng

Sanders

Morgan

et al. 2017

Sci Rep

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Wound healing and the management of chronic wounds represent a significant burden on the NHS. Members of the suppressor of cytokine signalling (SOCS) family have been implicated in the regulation of a range of cellular processes. The current study aims to explore the importance of SOCS-3 and SOCS-4 in regulating cellular traits associated with wound healing. SOCS-3 over-expression and SOCS-4 knockdown mutant lines were generated and verified using q-PCR and western blotting in human keratinocytes (HaCaT) and endothelial cells (HECV). Over-expression of SOCS-3 resulted in a significantly reduced proliferative rate in HaCaT keratinocytes and also enhanced the tubule formation capacity of HECV cells. SOCS-4 knockdown significantly reduced HaCaT migration and HECV cell tubule formation. Suppression of SOCS-4 influenced the responsiveness of HaCaT and HECV cells to EGF and TGFβ and resulted in a dysregulation of phospho-protein expression in HaCaT cells. SOCS-3 and SOCS-4 appear to play regulatory roles in a number of keratinocyte and endothelial cellular traits associated with the wound healing process and may also be able to regulate the responsiveness of these cells to EGF and TGFβ. This implies a potential regulatory role in the wound healing process and, thus highlights their potential as novel therapies.

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“…Mature microRNAs (miRNAs) and mRNA levels was detected by using qRT-PCR assays as described 37 . For miRNAs, total RNA was isolated using a mirVana miRNA isolation kit (Ambion).…”

Section: Quantitative Real-time Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

Long Noncoding RNA CTC Inhibits Proliferation and Invasion by Targeting miR-146 to Regulate KIT in Papillary Thyroid Carcinoma

Liao¹,

Shi²,

Liu³

et al. 2020

Sci Rep

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Several lines of evidence have shown that long non-coding RNAs (lncRNAs) are dysregulated in many diseases. nevertheless, the biological relevance of the lncRnAs in papillary thyroid carcinoma (ptc) has not been fully explored. We demonstrated that CTC was a negative regulator of PTC cell migration and invasion in vitro and in vivo. We found that microRNA-146 (miR-146) is an inhibitory target of CTC. We then demonstrated that CTC functioned as a miR-146 decoy to de-repress expression of KIT. Further study demonstrated that CTC modulated the progression and chemoresistance of PTC cells via miR-146 and KIT. The analysis of hundreds of clinical specimens revealed that CTC and KIT levels were downregulated, whereas miR-146 levels were greater in PTC tissues than in normal thyroid. Their expression levels correlated with one another. In conclusion, CTC functions as a competing endogenous RNA to inhibit the progression and chemoresistance of PTC cells, and identifies CTC serve as a potential therapeutic agent to suppress PTC progression. Thyroid carcinomas represent the majority of endocrine neoplasms worldwide, and their incidence has increased steadily over the past decade 1-3. Thyroid cancers can be classified as three types: well-differentiated, poorly differentiated and anaplastic carcinomas 4,5. Papillary thyroid carcinoma (PTC) is a well-differentiated type, accounting for 70-80% of thyroid carcinomas 4,5. Through surgical removal, thyroid hormone and adjuvant radioactive iodine therapy, PTC has a favorable prognosis 6. Nevertheless, some patients exhibit aggressive behavior due to metastases 6. Therefore, identification of potential biomarkers and therapeutic targets could aid the therapy of PTC. The non-coding RNA (ncRNA) refers to RNA molecules that cannot encode proteins, but nevertheless have biological functions 7,8. These ncRNAs has many types, including microRNAs, snoRNAs and longer transcripts 9,10. Long non-coding RNAs (LncRNAs) (>200 nt) are a newly-described class of RNA transcripts which had been regard as "transcriptional noise" in the past 11,12. Nevertheless, several lines of data support the notion that particular lncRNAs function as crucial regulators in the cancer biology, rather than "nonsense fragments" 13-15. For PTC, many functional lncRNAs have been characterized, including ATB, CASC2, H19, and NEF, suggesting that lncRNAs may be able to be diagnostic tags and potential therapeutic targets 16-20. MircroRNAs (miRNAs) are noncoding RNA molecules in 18-25 nucleotides length that negatively regulate gene expression at the post-transcriptional level 21,22. miRNAs regulate proliferation, metastasis and apoptosis by inhibiting tumor suppressor gene pathways or activating oncogenic pathways 23. Almost half of human miRNAs are in the position of fragile sites and genomic regions that are associated with cancers 24. Nevertheless, little is known about the biological role of miRNAs in PTC. A recent study reported that miR-146, miR-221 and miR-222 were upregulated in PTC compared to levels in adjacen...

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Interleukin-23 Facilitates Thyroid Cancer Cell Migration and Invasion by Inhibiting SOCS4 Expression via MicroRNA-25

Cited by 31 publications

References 33 publications

MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation

MicroRNA-548-3p and MicroRNA-576-5p enhance the migration and invasion of esophageal squamous cell carcinoma cells via NRIP1 down-regulation

In vitro significance of SOCS-3 and SOCS-4 and potential mechanistic links to wound healing

Long Noncoding RNA CTC Inhibits Proliferation and Invasion by Targeting miR-146 to Regulate KIT in Papillary Thyroid Carcinoma

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