Interleukin 3 activates human blood basophils via high-affinity binding sites.

Valent, Peter; Besemer, J; Muhm, Manfred; Majdic, Otto; Lechner, Klaus; Bettelheim, Peter

doi:10.1073/pnas.86.14.5542

Cited by 191 publications

(171 citation statements)

References 34 publications

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“…In the human system, the most potent basophil differentiation factor is interleukin‐3 (IL‐3) 29, 30, 31. This growth factor has been described to induce basophil differentiation and maturation in hematopoietic stem and progenitor cells, but also promotes the viability and activation of mature blood basophils 29, 30, 31, 32. Other basophil growth regulators include granulocyte/macrophage colony‐stimulating factor (GM‐CSF), IL‐5, transforming growth factor‐beta (TGF‐ß) and thymic stromal lymphopoietin (TSLP) 33, 34, 35.…”

Section: Basophil Differentiation In Healthy Bm and In Ph+ CMLmentioning

confidence: 99%

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The underestimated role of basophils in Ph⁺ chronic myeloid leukaemia

Valent

Horny

Arock

2018

Eur J Clin Investigation

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Chronic myeloid leukaemia (CML) is a hematopoietic neoplasm defined by the chromosome translocation t(9;22) and the related oncogene, BCR‐ABL1. In most patients, leukaemic cells can be kept under control using BCR‐ABL1‐targeting drugs. However, many patients relapse which remains a clinical challenge. In particular, patients with advanced (accelerated or blast phase) CML have a poor prognosis. So far, little is known about molecular and cellular interactions and features that contribute to disease progression and drug resistance in CML. One key prognostic factor at diagnosis is marked basophilia. However, although basophils are well‐known multifunctional effector cells, their impact in CML remains uncertain. In this article, we discuss the potential role of basophils as active contributors to disease evolution and progression in CML. In particular, basophils serve as a unique source of inflammatory, angiogenic and fibrogenic molecules, such as vascular endothelial growth factor or hepatocyte growth factor. In addition, basophils provide vasoactive substances, like histamine as well as the cytokine‐degrading enzyme dipeptidyl‐peptidase IV which may promote stem cell mobilization and the extramedullary spread of stem and progenitor cells. Finally, basophils may produce autocrine growth factors for myeloid cells. Understanding the role of basophils in CML evolution and progression may support the development of more effective treatment concepts.

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Section: Basophil Differentiation In Healthy Bm and In Ph+ CMLmentioning

confidence: 99%

“…In mature basophils, additional factors and molecules, such as complement factors (C3a, C5a) are involved in the regulation of survival, migration, adhesion and activation 36. Most of these cytokines are considered to act on CML basophils in the same way as on normal basophils 27, 32, 36…”

Section: Basophil Differentiation In Healthy Bm and In Ph+ CMLmentioning

confidence: 99%

The underestimated role of basophils in Ph⁺ chronic myeloid leukaemia

Valent

Horny

Arock

2018

Eur J Clin Investigation

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“…Basophil Histamine-Release Experiments. Granulocytes from birch pollen-allergic donors were isolated by dextran sedimentation from heparinized blood samples (21). Different concentrations (0.001, 0.01, 0.1, 1, and 10 g͞ml) of rBet v 1, rAln g 1, and (for control purposes) an immunologically unrelated allergen (i.e., timothy grass pollen allergen, Phl p 1) or anti-IgE Abs were incubated with sera obtained before and after treatment and then exposed to the granulocyte preparation (22)(23)(24).…”

Section: Methodsmentioning

confidence: 99%

Vaccination with genetically engineered allergens prevents progression of allergic disease

Niederberger

Horak

Vrtala

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

332

316

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IgE-mediated allergy affects >25% of the population in industrialized countries. Repeated contact with the disease-eliciting allergens induces rises of allergen-specific IgE Abs and progression of the disease to more severe manifestations. Our study uses a type of vaccine that is based on genetically modified allergen derivatives to treat allergic patients. We developed hypoallergenic derivatives of the major birch pollen allergen, Bet v 1, by genetic engineering and vaccinated birch pollen-allergic patients (n ‫؍‬ 124) in a double-blind, placebo-controlled study. Active treatment induced protective IgG Abs that inhibited allergen-induced release of inflammatory mediators. We also observed a reduction of cutaneous sensitivity as well as an improvement of symptoms in actively treated patients. Most important, rises of allergen-specific IgE induced by seasonal birch pollen exposure were significantly reduced in vaccinated patients. Vaccination with genetically engineered allergen derivatives is a therapy for allergy that not only ameliorates allergic reactions but also reduces the IgE production underlying the disease.

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“…Cells were incubated with increasing concentrations of recombinant carp parvalbumin, anti-human IgE Ab, or buffer as previously described (38). Liberated histamine was measured in the cell-free supernatants by RIA (Immunotech, Marseille, France).…”

Section: Basophil Histamine Release Assaymentioning

confidence: 99%

Recombinant Carp Parvalbumin, the Major Cross-Reactive Fish Allergen: A Tool for Diagnosis and Therapy of Fish Allergy

Swoboda¹,

Bugajska-Schretter²,

Verdino

et al. 2002

The Journal of Immunology

220

168

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IgE-mediated reactions to fish allergens represent one of the most frequent causes of food allergy. We have constructed an expression cDNA library from carp (Cyprinus carpio) muscle in phage λgt11 and used serum IgE from a fish allergic patient to isolate 33 cDNA clones that coded for two parvalbumin isoforms (Cyp c 1.01 and Cyp c 1.02) with comparable IgE binding capacities. Both isoforms represented calcium-binding proteins that belonged to the β-lineage of parvalbumins. The Cyp c 1.01 cDNA was overexpressed in Escherichia coli, and rCyp c 1.01 was purified to homogeneity. Circular dichroism analysis and mass spectroscopy showed that rCyp c 1.01 represented a folded protein with mainly α-helical secondary structure and a molecular mass of 11,416 Da, respectively. rCyp c 1.01 reacted with IgE from all fish-allergic patients tested (n = 60), induced specific and dose-dependent basophil histamine release, and contained most of the IgE epitopes (70%) present in natural allergen extracts from cod, tuna, and salmon. Therefore, it may be used to identify patients suffering from IgE-mediated fish allergy. The therapeutic potential of rCyp c 1.01 is indicated by our findings that rabbit Abs raised against rCyp c 1.01 inhibited the binding of IgE (n = 25) in fish-allergic patients to rCyp c 1.01 between 35 and 97% (84% mean inhibition) and that depletion of calcium strongly reduced IgE recognition of rCyp c 1.01. The latter results suggest that it will be possible to develop strategies for immunotherapy for fish allergy that are based on calcium-free hypoallergenic rCyp c 1.01 derivatives.

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Interleukin 3 activates human blood basophils via high-affinity binding sites.

Cited by 191 publications

References 34 publications

The underestimated role of basophils in Ph⁺ chronic myeloid leukaemia

The underestimated role of basophils in Ph⁺ chronic myeloid leukaemia

Vaccination with genetically engineered allergens prevents progression of allergic disease

Recombinant Carp Parvalbumin, the Major Cross-Reactive Fish Allergen: A Tool for Diagnosis and Therapy of Fish Allergy

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Interleukin 3 activates human blood basophils via high-affinity binding sites.

Cited by 191 publications

References 34 publications

The underestimated role of basophils in Ph+ chronic myeloid leukaemia

The underestimated role of basophils in Ph+ chronic myeloid leukaemia

Vaccination with genetically engineered allergens prevents progression of allergic disease

Recombinant Carp Parvalbumin, the Major Cross-Reactive Fish Allergen: A Tool for Diagnosis and Therapy of Fish Allergy

Contact Info

Product

Resources

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The underestimated role of basophils in Ph⁺ chronic myeloid leukaemia

The underestimated role of basophils in Ph⁺ chronic myeloid leukaemia