Interleukin 3 and cell cycle progression

Kelvin, David J.; Chance, Susan E.; Shreeve, Mona M.; Axelrad, Arthur A.; Connolly, Joe A.; McLeod, Donald W.

doi:10.1002/jcp.1041270308

Cited by 36 publications

(17 citation statements)

References 38 publications

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“…IL-3 is thought to be necessary both for the continued proliferation of hematopoietic multipotential cells (46) and for the continued viability of cells that reside in Go and/or GI phase of the cell cycle (47). IL-3 alone was able to sustain hematopoiesis in the suspension culture system for 4 or 5 wk, while combinations ofIL-I or IL-6 and IL-3 extended hematopoietic activity to 8 wk.…”

Section: Discussionmentioning

confidence: 99%

Cytokine-dependent long-term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow.

Brandt¹,

et al. 1990

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Human marrow cells positive for the CD34 antigen but not expressing HLA-DR, CD15, or CD71 antigens were isolated. In a liquid culture system supplemented with 48-hourly additions of recombinant interleukins IL-la, IL-3, IL-6, or granulocyte/macrophage colony-stimulating factor (GM-CSF), these cells were capable of sustaining in vitro hematopoiesis for up to eight weeks. The establishment of an adherent cell layer was never observed. Cultures containing no exogenous cytokine produced clonogenic cells for only 1 wk. IL-la and IL-6 were alone able to support hematopoiesis for 2 or 3 wk. Cells maintained with GM-CSF proliferated and contained assayable colony-forming cells for 3 or 4 wk, while maximal cellular expansion and generation of assayable progenitor cells occurred in the presence of IL-3 for 4-5 wk. When IL-3 was combined with IL-la or IL-6, hematopoiesis was sustained for 8 wks. Basophil numbers were markedly increased in the presence of IL-3. These studies indicate that marrow subpopulations can sustain hematopoiesis in vitro in the presence of repeated additions of cytokines. We conclude that a major function of marrow adherent cells in long-term cultures is that of providing cytokines which promote the proliferation and differentiation of primitive hematopoietic cells. (J. Clin. Invest. 1990. 86:932-941.)

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Section: Discussionmentioning

confidence: 99%

Cytokine-dependent long-term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow.

Brandt¹,

et al. 1990

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“…The culture medium was aspirated and replaced with fresh medium without PWM-SCM every 2 days. Synchronization of mast cells at the G1 phase of the cell cycle was carried out by culturing mast cells in the absence of PWM-SCM for 24 hr (12,15). Mast cells were identified by staining a cytocentrifuge preparation of trypsinized cultures with Alcian blue.…”

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confidence: 99%

In vitro duplication and in vivo cure of mast-cell deficiency of Sl/Sld mutant mice by cloned 3T3 fibroblasts.

Fujita

Onoue²,

Ebi³

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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S1/Sd mutant mice are profoundly deficient in tissue mast cells as a result of a defect in the microenvironment promoting the development ofthese cells. To facilitate the analysis of the SI mutation, we attempted to establish an in vitro system in which the in vivo defect of S/1Sd mice could be A homogenous population of mast cells has been generated in vitro by cultivating progenitors from bone marrow in the presence of a T-cell-derived growth factor, interleukin (IL) 3 (for reviews, see refs. 7 and 8). The action of IL-3 has been shown to be enhanced by another T-cell-derived growth factor, IL-4 (9, 10). Recently we described another mode of mast-cell growth, which depends on contact with fibroblasts (11). When mast cells obtained in vitro from bone-marrow cells were cocultured with the NIH 3T3 fibroblast cell line, the mast cells were maintained in the absence of an exogenous source of IL-3 and IL-4. We further demonstrated that cultured mast cells derived from W/WV mice were defective in fibroblast-dependent growth, although they responded to T-cell-derived growth factors as efficiently as those from +/+ mice (12).In the present study we examined whether the mast-cell deficiency in 51/51d mice could be reproduced in vitro by a similar coculture system. No abnormality in proliferative response to either T-cell-derived Nakahata et al. (13). Homogenous populations of mast cells were obtained by culturing bone-marrow cells at 106 cells per ml in a-minimal essential medium supplemented with 10-4 M 2-mercaptoethanol/20% (vol/vol) horse serum/10% (vol/vol) PWM-SCM. Half of the medium was replaced every 7 days. Four weeks after initiation of the culture, >98% of the cultured cells were identified as mast cells, and 13% of them were found to be in S phase as described (11). Therefore, those cultured for >4 wk were used in these experiments. The NIH 3T3 fibroblast cell line derived from a Swiss mouse embryo (14) or other fibroblast cell lines were cocultured with mast cells in the absence of PWM-SCM as described (11,12). Briefly, 105 cultured mast cells suspended in 2 ml of medium containing 5% fetal calf serum but not PWM-SCM were added to a confluent culture Abbreviations: IL, interleukin; PWM-SCM, pokeweed mitogenstimulated spleen cell-conditioned medium; BrdUrd, 5-bromodeoxyuridine. *To whom reprint requests should be addressed. 2888The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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“…However an effect of such factors like Interleukin 3 0L-3), Interleukin 4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in the G0/Gl-phase or at the G1/S boarder has been demonstrated by several groups (Plutznik et al, 1984;Kelvin et al, 1986;London and McKearn, 1987). In the G1/S transition model by Auger (see in this volume) growth factors control the production of an initiator of DNA replication.…”

Section: Introductionmentioning

confidence: 99%

Measurement of efflux from G1-phase in a growth factor dependent cell line

Ellwart¹,

Dormer²

1992

Acta Biotheor

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In order to test a mathematical model of G1/S-phase transition, the proliferative response of the murine myeloid interleukin 3 (IL-3) dependent cell line NFS-78 to graded reduction of IL-3 levels was measured. Exponentially growing cells were exposed to bromodeoxyuridine (BUdR), which replaces thymidine (TdR) in the DNA double strands during DNA synthesis. After incubation periods ranging from 3 to 36 h the cells were fixed and stained with a fluorescence dye mixture of Hoechst 33258 and ethidium bromide (EB) and subsequently analyzed in a two-parametrical flow cytometer. The BUdR-quenched TdR-specific Hoechst 33258 fluorescence of each cell provides information on the cell cycle location at the start of incubation and on whether or not a cell has divided. The DNA-specific EB fluorescence provides information on the actual cell cycle location at the end of the incubation period. From the 2-dimensional fluorescence distributions the efflux from G1-phase was calculated. Upon IL-3 reduction the cells showed accumulation in the G1-phase along with a reduction in the progression rate through the other phases of the cell cycle. By staining with the vital dye Hoechst 33342 as well as with propidium iodide (PI) it was further possible to show that cell death after IL-3 withdrawal occurred in all phases of the cell cycle.

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Interleukin 3 and cell cycle progression

Cited by 36 publications

References 38 publications

Cytokine-dependent long-term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow.

Cytokine-dependent long-term culture of highly enriched precursors of hematopoietic progenitor cells from human bone marrow.

In vitro duplication and in vivo cure of mast-cell deficiency of Sl/Sld mutant mice by cloned 3T3 fibroblasts.

Measurement of efflux from G1-phase in a growth factor dependent cell line

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