Interleukin-3 and interleukin-1 alpha allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1

Yq, Zhou; Stanley, E. Richard; Clark, SC; Hatzfeld, Jacques; Levesque, Jean‐Pierre; Federici, Christian; Watt, SM; Hatzfeld, Antoinette

doi:10.1182/blood.v72.6.1870.bloodjournal7261870

Cited by 24 publications

(7 citation statements)

References 27 publications

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“…Notwithstanding these variations in CSF responses among the groups of mice, we observed a fourto fivefold increase in the number of granulocyte-macrophage CFC in the spleens of all mice given IL-1, whether they were normal or acutely or chronically infected. In the case of the chronically infected mice, either IL-1 was acting on the CFC to make them more sensitive to CSF, as reported previously (21), or the lesser increase in CSF in these mice was still sufficient to increase their CFC. The effect on bone marrow CFC was slight.…”

Section: Discussionsupporting

confidence: 58%

“…The fact that the increase in CSF-1 was detected by the radioimmunoassay is important, since the radioimmunoassay, unlike bioassays, is not influenced by synergistic or inhibitory effects. IL-1 can act synergistically with some CSFs in vitro (21), and the apparent rise in total CSA could have been attributed to residual IL-1. Similarly, the increase in CSF-1 could not be due to removal of an inhibitor, which has been reported with a CFC assay (1).…”

Section: Discussionmentioning

confidence: 99%

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Prophylaxis or treatment of experimental brucellosis with interleukin-1

1991

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Intravenously injected recombinant human interleukin-1 alpha (IL-la) given to mice 4 h before infection with BruceUla abortus 19 depressed the growth of bacteria in the spleen and liver. However, the same dose (105 U) or a 10-fold higher dose was not able to decrease numbers of bacteria when given to chronically infected mice. IL-1 injected into normal mice induced a dramatic increase 2 h later in colony-stimulating activity in serum, measured by bone marrow proliferation, and in colony-stimulating factor 1, measured by radioimmunoassay. Colony-sthnulating factor levels declined but remained higher than normal for at least 12 h. The early peak stimulation was not observed in chronically infected mice, but the more prolonged elevation was. As a result of IL-1 treatment, the number of colony-forming cells, especially in the spleen, was increased in normal and acutely or chronically infected mice. Myeloperoxidase staining of newly formed monocytes and polymorphonuclear cells in the spleen revealed an increase in the number of these cells in normal and acutely infected mice as a result of IL-1 treatment, but there was no increase in the already high numbers in chronically infected mice. The relationship between these observations and the basis of chronic infection are discussed.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Prophylaxis or treatment of experimental brucellosis with interleukin-1

1991

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“…CSF-1 decreased the number of c-fms positive immature cells We have shown that 20 ng/ml CSF-1 is the optimal concentration for supporting the division and maturation of monocytes and macrophages (Zhou et al, 1988). When 20 ngiml CSF-1 was added with IL3 to primary cultures of CD34-t cells, we observed, as shown in Figure 2, only half as many c-fms positive cells as in comparable cultures supported with IL3 alone.…”

Section: C-fms Is Expressed In a Subpopulation Of Human Bone Marrow Pmentioning

confidence: 67%

“…IL3 (1.7 U/ml) and CSF-1 (1 or 20 ng/ml) were added a t the specified concentrations. It should be noted that 1 ng of CSF-1 in this study corresponds to 1 U of recombinant human CSF-1 and to 50 U of CSF-1 in our previous study (Zhou et al, 1988). Anti-CSF-1 antiserum was used a t a final dilution 1:50.…”

Section: Colony Assaysmentioning

confidence: 91%

“…In the human system, we Q 1993 WILEY-LISS, INC. have shown that CSF-1 alone will trigger mainly the most mature monocytic progenitors only when added at high concentrations (20 ng/ml) to cultures of human bone marrow progenitors. When added in combination with IL1 or IL3, low concentrations of CSF-1 (1 ng/ml), which alone had no effect, could trigger immature progenitors to develop towards the monocytic lineage (Zhou et al, 1988). These observations in the mouse and human systems suggested that the c-fms protooncogene, which encodes the CSF-1 receptor (Sherr et al, 1985;Sherr, 1990), could be expressed on progenitors grown in culture with IL1 or IL3.…”

mentioning

confidence: 98%

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CSF‐1 control of C‐FMS expression in normal human bone marrow progenitors

Panterne¹,

Zhou²,

Hatzfeld³

et al. 1993

Journal Cellular Physiology

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We have previously shown (Zhou et al: Blood, 72:1870, 1988) that IL3, added with low concentrations of CSF-1 (1 ng/ml) to normal human CD34+ enriched cells, promoted the development of various types of colonies including those containing immature monocytes. However, when high concentrations of CSF-1 (20 ng/ml) were added alone or together with IL3, smaller colonies with mature macrophages were found. Here we show by in situ hybridization that IL3 allows the development, from CD34+ cells, of a subpopulation of immature progenitors which express the CSF-1 receptor (c-fms) mRNA. The expression of c-FMS protein was also substantiated by immunocytochemical studies using anti-c-fms antibody. The percentage of c-fms positive cells peaked at day 7 and began to decrease thereafter. When anti-CSF-1 antibodies were included in the culture, the decrease in c-fms mRNA after day 7 was abrogated. This indicated that endogenous CSF-1 was produced as CD34+ cells developed into monocytes or progenitors of monocytes and that CSF-1 modulates c-fms expression. We further demonstrated that when a high dose of CSF-1 (20 ng/ml) was added at day 7 to IL3-stimulated CD34+ cells, a rapid down-regulation of c-fms mRNA and protein was seen. No down-regulation was observed with low concentration of CSF-1 (1 ng/ml). The possibility that different concentrations of CSF-1 could modulate the development of monocytic progenitors is discussed.

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Comparative analysis of the influences of IL-1, IL-3 and GM-CSF on the commitment of granulocyte-macrophage progenitors in vitro

Maciejewski

Weber²,

Neuhaus

et al. 1991

Ann Hematol

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Our experiments were directed towards the detection of the influence of interleukin-1 (IL-1); interleukin-3 (IL-3), and granulocyte-macrophage colony-stimulating factor (GM-CSF) on the generation of granulocyte-macrophage progenitor cells. We also set out to examine whether this process is connected with changes within the early precursor cell compartment. Bone marrow suspension cultures (12 days) supplemented with these cytokines were tested for the presence of GM colony-forming cells (GM-CFC) in a colony-forming unit assay. The percentage of CD34+ and HLA-DR+ as well as the number of blasts and promyelocytes were estimated cytofluorometrically and morphologically. The proliferative effect of GM-CSF was associated with a net increase of GM-CFC and HLA-DR+ myeloid cells and a decrease in the percentage of CD34+ early precursor cells. IL-3 acted similarly and also caused an absolute decrease of CD34+ cells in the cultures. IL-1 did not stimulate the generation of blasts or GM-CFC but elevated the number of CD34- as well as HLA-DR-expressing cells in the cultures. These results imply that GM-CSF supported the maintenance of hematopoiesis in vitro. The transition from early precursor cells to committed myeloid progenitor cells (GM-CFC) and more mature precursor cells (G-CFC, M-CFC) may be supported by GM-CSF without affecting the self-renewing capacity of CD34+ early precursors. In contrast, the blast-generating and proliferation-inducing action of IL-3 is associated with a drop in the total number of CD34+ stem cells. An efficient renewal of this population obviously depends on the presence of IL-1.

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Interleukin-3 and interleukin-1 alpha allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1

Cited by 24 publications

References 27 publications

Prophylaxis or treatment of experimental brucellosis with interleukin-1

Prophylaxis or treatment of experimental brucellosis with interleukin-1

CSF‐1 control of C‐FMS expression in normal human bone marrow progenitors

Comparative analysis of the influences of IL-1, IL-3 and GM-CSF on the commitment of granulocyte-macrophage progenitors in vitro

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