Interleukin-4-induced FABP4 promotes lipogenesis in human skeletal muscle cells by activating the PPAR γ signaling pathway

Wang, Xin-Wen; Sun, Yuan; Chen, Xiao; Zhang, Wenzhi

doi:10.1007/s12013-022-01063-7

Cited by 6 publications

(2 citation statements)

References 41 publications

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“…FABP4 also triggers the ubiquitination and subsequent proteasomal degradation of PPARγ ( Herroon et al, 2013 ). Furthermore, FABP4 induces IL-4-stimulated adipogenesis in human skeletal muscle cells through the activation of the PPARγ signaling pathway ( Wang et al, 2022 ). The correlation between FABP4 and PPARγ is also supported by our findings.…”

Section: Discussionmentioning

confidence: 99%

Proteomic profiling reveals the potential mechanisms and regulatory targets of sirtuin 4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s mouse model

Weng

Song

et al. 2023

Front. Neurosci.

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IntroductionParkinson’s disease (PD), as a common neurodegenerative disease, currently has no effective therapeutic approaches to delay or stop its progression. There is an urgent need to further define its pathogenesis and develop new therapeutic targets. An increasing number of studies have shown that members of the sirtuin (SIRT) family are differentially involved in neurodegenerative diseases, indicating their potential to serve as targets in therapeutic strategies. Mitochondrial SIRT4 possesses multiple enzymatic activities, such as deacetylase, ADP ribosyltransferase, lipoamidase, and deacylase activities, and exhibits different enzymatic activities and target substrates in different tissues and cells; thus, mitochondrial SIRT4 plays an integral role in regulating metabolism. However, the role and mechanism of SIRT4 in PD are not fully understood. This study aimed to investigate the potential mechanism and possible regulatory targets of SIRT4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice.MethodsThe expression of the SIRT4 protein in the MPTP-induced PD mouse mice or key familial Parkinson disease protein 7 knockout (DJ-1 KO) rat was compared against the control group by western blot assay. Afterwards, quantitative proteomics and bioinformatics analyses were performed to identify altered proteins in the vitro model and reveal the possible functional role of SIRT4. The most promising molecular target of SIRT4 were screened and validated by viral transfection, western blot assay and reverse transcription quantitative PCR (RT-qPCR) assays.ResultsThe expression of the SIRT4 protein was found to be altered both in the MPTP-induced PD mouse mice and DJ-1KO rats. Following the viral transfection of SIRT4, a quantitative proteomics analysis identified 5,094 altered proteins in the vitro model, including 213 significantly upregulated proteins and 222 significantly downregulated proteins. The results from bioinformatics analyses indicated that SIRT4 mainly affected the ribosomal pathway, propionate metabolism pathway, peroxisome proliferator-activated receptor (PPAR) signaling pathway and peroxisome pathway in cells, and we screened 25 potential molecular targets. Finally, only fatty acid binding protein 4 (FABP4) in the PPAR signaling pathway was regulated by SIRT4 among the 25 molecules. Importantly, the alterations in FABP4 and PPARγ were verified in the MPTP-induced PD mouse model.DiscussionOur results indicated that FABP4 in the PPAR signaling pathway is the most promising molecular target of SIRT4 in an MPTP-induced mouse model and revealed the possible functional role of SIRT4. This study provides a reference for future drug development and mechanism research with SIRT4 as a target or biomarker.

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Section: Discussionmentioning

confidence: 99%

Proteomic profiling reveals the potential mechanisms and regulatory targets of sirtuin 4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s mouse model

Weng

Song

et al. 2023

Front. Neurosci.

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show abstract

“…Besides, there are many articles reporting the involvement of regulation of actin cytoskeleton and PPAR signaling pathway in muscle development. Regulation of actin cytoskeleton is related to cell migration, and PPAR signaling pathway is involved in adipogenesis of skeletal muscle cell ( Tang and Gerlach, 2017 ; Wang et al, 2017b , Wang et al, 2022b ). Therefore, these pathways can all affect the development of skeletal muscles.…”

Section: Discussionmentioning

confidence: 99%

Characterization and comparative transcriptomic analysis of skeletal muscle in female Pekin duck and Hanzhong Ma duck during different growth stages using RNA-seq

Cao,

Cai,

et al. 2023

Poultry Science

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Changes to PUFA-PPAR pathway during mesaconitine induced myocardial coagulative necrosis

Chen,

Deng,

Zhang

et al. 2023

Food and Chemical Toxicology

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Interleukin-4-induced FABP4 promotes lipogenesis in human skeletal muscle cells by activating the PPAR γ signaling pathway

Cited by 6 publications

References 41 publications

Proteomic profiling reveals the potential mechanisms and regulatory targets of sirtuin 4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s mouse model

Proteomic profiling reveals the potential mechanisms and regulatory targets of sirtuin 4 in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s mouse model

Characterization and comparative transcriptomic analysis of skeletal muscle in female Pekin duck and Hanzhong Ma duck during different growth stages using RNA-seq

Changes to PUFA-PPAR pathway during mesaconitine induced myocardial coagulative necrosis

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