Interleukin-4 production in BALB/c mice immunized with Anisakis simplex

Perteguer, María Jesús; Cuéllar, C.

doi:10.1590/s0074-02762001000700016

Cited by 3 publications

(7 citation statements)

References 7 publications

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“…138 IgG1 was the predominant IgG isotype produced, 80,138 and Th2 cytokines were induced in wild-type mice. 80,145 Re-infection caused an increase in specific IgE antibodies in both rats and mice, indicating that multiple infections with Anisakis larvae promote IgE production. 80,88,136 Only live larvae were found to induce antibodies in rats, 146 supporting the idea that allergic sensitization requires live infection in humans.…”

Section: Animal Studies Of Anisakis Immunologymentioning

confidence: 99%

“…Antibodies were at first generated to excretory–secretory products of the larvae and later to somatic antigens after the larvae had been broken up . IgG1 was the predominant IgG isotype produced, and Th2 cytokines were induced in wild‐type mice . Re‐infection caused an increase in specific IgE antibodies in both rats and mice, indicating that multiple infections with Anisakis larvae promote IgE production .…”

Section: Introductionmentioning

confidence: 99%

“…Symptoms of Anisakis infection in humans are varied, ranging from asymptomatic or mild infection to gastrointestinal anisakiasis to gastroallergic anisakiasis. The important of the genetic background of the host in the immune responses to Anisakis is demonstrated by the varying responses in mice of different strains and knockout mouse strains . Wild‐type BALB/c mice mount a Th2 response to infection, associated with allergic symptoms after subsequent exposure to Anisakis proteins, whereas IL‐4Rα‐deficient mice mount a Th1 response to infection have increased levels of infiltrating immune cells in the intraperitoneal cavity and show no signs of allergic disease upon exposure to Anisakis proteins .…”

Section: Introductionmentioning

confidence: 99%

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Anisakis – immunology of a foodborne parasitosis

Nieuwenhuizen

2016

Parasite Immunology

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SummaryAnisakis species are marine nematodes which can cause zoonotic infection in humans if consumed in raw, pickled or undercooked fish and seafood. Infection with Anisakis is associated with abdominal pain, nausea and diarrhoea and can lead to massive infiltration of eosinophils and formation of granulomas in the gastrointestinal tract if the larvae are not removed. Re-infection leads to systemic allergic reactions such as urticarial or anaphylaxis in some individuals, making Anisakis an important source of hidden allergens in seafood. This review summarizes the immunopathology associated with Anisakis infection. Anisakiasis and gastroallergic reactions can be prevented by consuming only fish that has been frozen to −20°C to the core for at least 24 hours before preparation. Sensitization to

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Section: Animal Studies Of Anisakis Immunologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Anisakis – immunology of a foodborne parasitosis

Nieuwenhuizen

2016

Parasite Immunology

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“…Although the humoral immune response to A simplex has been characterized in both animals and humans, to date there are hardly any references on how larval products of A simplex interact with cellular immunity. A better understanding of the mechanisms by which this parasite interferes with the host's immune system would provide new data on allergies and knowledge of how to combat them.…”

Section: Resultsmentioning

confidence: 99%

Induction of tolerogenic properties by Anisakis larval antigens on murine dendritic cells

Zamora

Rodero

Andreu‐Ballester

et al. 2019

Parasite Immunology

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Summary Aims The objective of this work is to investigate whether Anisakis simplex larval antigens present immunomodulatory properties by the induction of tolerogenic dendritic cells (DCs) from two strains of mice (BALB/c and C57BL/6J). Methods and Results We used mouse bone marrow‐derived DCs. We determined their antigen‐presenting ability by expression of membrane markers (MHC I and MHC II, CD80, CD86) and intracellular expression levels of IL‐10 and IL‐12 cytokines. We also analysed whether stimulation with A simplex larval antigens is enhanced by the co‐administration of the TLR4 and TLR9 agonists [LPS E coli 026B6 and CpG (ODN1826), respectively]. Two differential types of responses were found in the two mouse strains studied: the BALB/c strain showed an acute and inflammatory response, whereas the C57BL/6J mice developed a more discrete and resistant response. This suggests the coexistence of two opposing responses generated by A simplex larval antigens and confirms that the host genetic basis plays a role in the development of a Th2 or Treg response. Conclusion The study of the mechanisms by which Anisakis manipulates the immune response through anti‐inflammatory molecules is of interest not only for the direct application on the development of anthelmintic strategies, but also for the development of new anti‐inflammatory products.

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“…Group II: Experimental group, included 30 mice which were inoculated orally with Anisakis antigen by intra-gastric gavage [15,19]. The dose was calculated to be 0.1 ml/mouse (about three larvae/mouse) according to a pilot study.…”

Section: Animalsmentioning

confidence: 99%

A Study of Allergic Sensitization to Anisakis Species in Experimental Mice

Diab¹,

Temsahy²,

Elkerdany³

et al. 2011

J Bacteriol Parasitol

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Background: Hypersensitivity to Anisakis species is a worldwide medical problem. The aim of this study was to detect the immunological response in experimental mice, through measurement of Immunoglobulin E (IgE) antibodies in their lymphocytes by flow cytometry, following the ingestion of Anisakis crude antigen.Method: Sixty Swiss albino mice were divided equally into control and experimental groups and each of them were further subdivided into five subgroups, six mice each. The percentage of IgE antibodies was measured in the lymphocytes of their splenic suspensions at zero, 1 st , 3 rd , 5 th and 7 th weeks post inoculation using Fluorescein isothiocyanate (FITC) anti -mouse IgE and were analyzed on a FACS Calibur flow cytometer Becton Dickinson equipped with an argon-ion laser apparatus operating at 488 nm. Results:The percentage of IgE antibodies was enhanced in lymphocytes of animals exposed to Anisakis antigen from the first week, peaking three weeks following initial exposure, starting a decline by week five and decreased more by the seventh week, as compared to the control group. Conclusion:The results obtained from this study proved the efficacy of flow cytometry in detecting the sensitization against Anisakis species through the measurement of IgE antibodies.

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Interleukin-4 production in BALB/c mice immunized with Anisakis simplex

Abstract: We investigated the interleukin

Cited by 3 publications

References 7 publications

Anisakis – immunology of a foodborne parasitosis

Anisakis – immunology of a foodborne parasitosis

Induction of tolerogenic properties by Anisakis larval antigens on murine dendritic cells

A Study of Allergic Sensitization to Anisakis Species in Experimental Mice

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