Interleukin 4 promotes expression of mast cell ICAM-1 antigen.

Abstract

Cell recognition molecules play a crucial role in the regulation of immune cells. We recently found that mast cells (MCs) express leukocyte recognition molecules, including ICAM-1 antigen, a natural ligand of LFA-1. We here report that interleukin 4 (IL-4), a pleiotropic cytokine and mast cell differentiation factor, selectively promotes expression of surface ICAM-l antigen and ICAM-1 mRNA in human MCs. IL-4 also up-regulates ICAM-1 antigen in cells of monocyte/macrophage lineage but has no effect on ICAM-1 antigen expressed on basophils, fibroblasts, or lymphocytes. The increase in expression of mast cell/macrophage ICAM-1 antigen induced by IL-4 may contribute to the accumulation of leukocytes and facilitate cell-contact-dependent regulation of immune cells in infammed tissues.Mast cells (MCs) are primarily located in mucosal and perivascular areas of various tissues. They are known to play an important role in allergic events and immune reactions and may accumulate at sites of inflammation together with attracted leukocytes (1). Increasing evidence suggests that cell surface molecules play a significant role in the accumulation, distribution, and regulation of leukocytes in inflammatory reactions (2). We recently found that MCs express leukocyte recognition molecules such as Pgp-1 antigen (CD44) or ICAM-1 antigen (CD54) (3).Interleukin 4 (IL-4) is a pleiotropic immunomodulator active on various cells of hemopoietic origin (4). Previous studies have shown that IL-4 is a differentiation factor for MCs (5,6). We here provide evidence that IL-4, in addition, is involved in the regulation of MC ICAM-1 antigen.MATERIALS AND METHODS Cells. The human mast cell line HMC-1 was established from a patient suffering from MC leukemia (7). Other cell lines used are listed in Table 2. Primary cells were obtained from patients by standard techniques after informed consent was given. MCs were enriched from lung (bronchiogenic carcinoma, n = 4), juvenile foreskin (n = 3), ascitic fluid (n = 4), and gastrointestinal mucosa (n = 2). Lung MC (n = 2) and chronic granulocytic leukemia (CGL) basophils (n = 2) were highly purified (90-98% purity) by negative selection using monoclonal antibodies (mAbs) and complement (8). Mononuclear blood cells (MNCs) were obtained from two normal donors, from two chronic lymphocytic leukemia patients, and from two CGL patients. In addition, tonsillar B cells, cultured umbilical vein endothelial cells, and lung fibroblasts were analyzed. Cells were cultured in complete RPMI medium supplemented with 1o fetal calf serum in a humidified atmosphere of 5% CO2 in air and 37°C.Cytokines. Recombinant human IL-4 (rhIL-4) expressed in Chinese hamster ovary cells was purified by ion-exchange chromatography essentially as described by Widmer et al. (9). Purified rhIL4 had a specific activity of 0.3 x 107 units/mg as defined by the amount of IL-4 required for half-maximal growth of T blasts precultured with 50 ng of rhIL-2 per ml and 1 ,ug of phytohemagglutinin per ml for 5 days. rhIL-2, rhIL-3, rhIL-8, ...

Expression of functional major histocompatility complex class II molecules on HMC-1 human mast cells

“…In this study, we demonstrate that MHC class II molecules are functionally expressed by HMC-1 human mast cells because we show that engagement of MHC class II molecules leads to ultrastructural changes, a characteristic of mast cell degranulation, and are able to activate T cells through presentation of SEA. In addition to the expression of MHC class II molecules, HMC-1 cells express a variety of adhesion molecules such as ICAM-1 and LFA-3 [71], providing adequate support for HMC-1 cells to present SAG to T cell in an efficient manner.…”

Cyclophilin A Interacts with HIV-1 Vpr and Is Required for Its Functional Expression

“…Total cell RNA was isolated using Trizol (Invitrogen), and 10 g of each RNA sample was separated in 1.2% denaturing agarose gel containing 17.8% formaldehyde. Following blotting and UV cross-linking to the nylon membrane (Hybond-N TM ; Amersham Biosciences) the membrane was pre-hybridized for 5 h at 65°C in Denhardt's solutions containing salmon sperm DNA and poly(A) RNA according to modification of a standard procedure described previously (43). For hybridization oligonucleotides (FLAG probe 5Ј-cttgtcgtcatcgtctttgtatgccat-3Ј, Vpr probe 5Ј-agtaacgcctattctgctatgtcgacacccaattctgaaatg-3Ј) were radioactively labeled with [␣-32 P]dCTP using terminal transferase (220 582; Roche Applied Science) and added to the hybridization solution for 12 h at 65°C.…”

Circulating intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in human malignancies

“…ICAM-1 expression has been reported to be increased by IL-1, '-and P-interferon, TNF-o, IL-4, IL-6 and IL-2 (Giavazzi et al, 1992;Buckle & Hogg, 1990;Valent et al, 1991), with effects being tissue-dependent. R.E.…”