Interleukin-6 (Il-6) in Seminal Plasma of Infertile Men, and Lipid Peroxidation of Their Sperm

Camejo, María Isabel; Segnini, A; Proverbio, Fulgencio

doi:10.1080/014850101316901280

Cited by 65 publications

(44 citation statements)

References 17 publications

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“…Interestingly, a dietary deficiency in essential fatty acids also decreases cAAT activity. 7 Because essential fatty acids down-regulate cytokine-induced lipid peroxidation, 36,37 deficiencies in essential fatty acids may promote acrolein-induced cAAT modification and inhibition. Further research is required to determine if acrolein modifies cAAT in vivo.…”

Section: Discussionmentioning

confidence: 99%

Acrolein Modifies and Inhibits Cytosolic Aspartate Aminotransferase

Southwell

Yeargans

Kowalewski

et al. 2002

Journal of Enzyme Inhibition and Medicinal Chemistry

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Acrolein is a reactive lipid peroxidation byproduct, which is found in ischemic tissue. We examined the effects of acrolein on cytosolic aspartate aminotransferase (cAAT), which is an enzyme that was previously shown to be inhibited by glycating agents. cAAT is thought to protect against ischemic injury. We observed that acrolein cross-linked cAAT subunits as evidenced by the presence of high molecular weight bands following SDS-PAGE. Acrolein-modified cAAT resisted thermal denaturation when compared with native cAAT. We also observed a decrease in intrinsic fluorescence (290 nm, ex; 380 nm, em). These observations are consistent with an acrolein-induced change in conformation that is more rigid and compact than native cAAT, suggesting that intramolecular cross-links occurred. Acrolein also inhibited activity, and the inhibition of enzyme activity correlated with the acrolein-induced formation of cAAT cross-links.

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Section: Discussionmentioning

confidence: 99%

Acrolein Modifies and Inhibits Cytosolic Aspartate Aminotransferase

Southwell

Yeargans

Kowalewski

et al. 2002

Journal of Enzyme Inhibition and Medicinal Chemistry

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“…The adverse effect of cytokines on sperm membrane properties might constitute one of the several mechanisms by which these immune factors interfere with sperm quality during semen bacterial infection. An increase in lipid sperm membrane peroxidation, as judged by MDA levels, has been demonstrated in the presence of some proinflammatory cytokines under both in situ [139,140] and in vitro conditions following the incubation of sperm with human recombinant proinflammatory cytokines [141,142]. However, the harmful effect of cytokines on sperm membranes appears to be closely associated with the accompanying leukocytospermia.…”

Section: Role Of the Immune/autoimmune Reactionsmentioning

confidence: 99%

Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen

Frączek

Kurpisz

2015

Folia Histochem Cytobiol.

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The invasion of the male reproductive tract by microorganisms, and its subsequent consequences for sperm fertilizing potential, has been intensely discussed. The role of the bacteria that are responsible for the colonization and contamination of the male urogenital tract, rather than its infection, in diminished sperm parameters raises the most controversy. There are numerous premises suggesting that bacterial semen infection is associated with male infertility. However, the molecular mechanism by which the fertility is affected is complex and multifactorial, and still presents a puzzle. Some authors have suggested that direct interactions between bacteria and human spermatozoa facilitate sperm immobilization, affect sperm morphology, and thus weaken the ability of sperm to fertilize. On the other hand, the massive infiltration of activated leukocytes into the inflammatory site may be associated with impairment of sperm fertilizing potential, due to oxidative, apoptotic, and immune processes. This review presents current research trends and aims to summarize the present knowledge of semen inflammation and causative bacterial agents in the male urogenital tract, with its consequence on seminological parameters, and male fertility status.

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“…Interleukin (IL)-6 is a multifunctional cytokine involved in numerous immunological proliferation processes in human sperm [7]. IL-6 was detected in the seminal plasma with higher levels in infertile men compared to those of fertile men, although the detailed effect of IL-6 was not fully known [1,4,10,12]. IL-8 is involved in intracellular signal transduction and adversely affects sperm membrane properties although the investigations of IL-8 with regard to reproduction are very limited [14].…”

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confidence: 99%