Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells

Mirkeshavarz, M; Ganjibakhsh, Meysam; Aminishakib, Pouyan; Farzaneh, Parvaneh; Mahdavi, Nazanin; Vakhshiteh, Faezeh; Karimi, A; Gohari, Neda Sadat; Kamali, Fahimeh; Kharazifard, Mohammad Javad; Fazeli, Seyed Abolhassan Shahzadeh; Nasimian, Ahmad

doi:10.14715/cmb/2017.63.10.21

Cited by 30 publications

(14 citation statements)

References 31 publications

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“…In this research, expression of VEGF protein in the cells was confirmed by the immunofluorescence study, previously described by Mirkeshavarz et al 19 The cells were fixed in 10% paraformaldehyde for 30 minutes at room temperature. After that, the cells were washed with PBS and blocked in 2% bovine serum albumin (BSA) at room temperature.…”

Section: Vegf Protein Expression In Cellsmentioning

confidence: 87%

Anti‐angiogenic efficacy of aflibercept and bevacizumab in primary oral squamous cell carcinoma cells

Ganjibakhsh

Monshizadeh

Nasimian

et al. 2018

J Oral Pathology Medicine

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Section: Vegf Protein Expression In Cellsmentioning

confidence: 87%

Anti‐angiogenic efficacy of aflibercept and bevacizumab in primary oral squamous cell carcinoma cells

Ganjibakhsh

Monshizadeh

Nasimian

et al. 2018

J Oral Pathology Medicine

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“…IL-6 is also a representative marker of clinical correlation and a common prognostic factor in patients with cancer. 25 Various studies have suggested that IL-6 is important in cervical carcinogenesis because it is a central pro-inflammatory cytokine involved in female genital infection and is abundant in the microenvironment of cervical cancer. 26 Moreover, the association of IL-6 with angiogenesis has been reported to depend on its ability to induce the production of VEGF, a potent angiogenic agent.…”

Section: Discussionmentioning

confidence: 99%

<p>CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells</p>

Zhou¹,

He²,

Zeng³

et al. 2020

OTT

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Tumor growth is one of the most lethal attributes of human malignancy. The expression of CCDC7, a novel gene which has multiple functions, has been shown to be associated with tumor growth and poor prognosis in patients with cancer. However, the specific functions of CCDC7 remain unclear. Here, we investigated the molecular mechanisms underlying the effects of CCDC7 on proliferation in cervical cancer. Materials and Methods: The MTT and EdU assays were performed to evaluate the function of CCDC7. The immunohistochemical, quantitative real-time PCR (qRT-PCR), ELISA and Western blot assay were used to detect the gene and protein expression in tissues and cells. A xenograft test was conducted to detect the impact of CCDC7 on tumor development in vivo. Results: In immunohistochemical analysis of 193 cases, normal cervical tissue and cervical cancer tissue show that CCDC7 expression is closely correlated with the development of cervical cancer and was positively correlated with the clinical stage and histological grade. Overexpression or knockdown of CCDC7 affected cell proliferation in cervical cancer cells in vitro. In a nude mouse xenograft model in vivo, knockdown of CCDC7 inhibited cell proliferation and tumor growth. Furthermore, CCDC7 overexpression upregulated interleukin (IL)-6 and vascular endothelial growth factor (VEGF) at mRNA and protein levels, and treatment with recombinant IL-6 or VEGF proteins also increased CCDC7 expression. In a case set of 80 patients with cervical cancer, we found that CCDC7, IL-6, and VEGF affected patient prognosis. Finally, inhibition of various signaling pathways using specific inhibitors indicated that CCDC7 blocked the decrease in cell proliferation observed following suppression of the JAK-STAT3 pathway, suggesting that CCDC7 functioned via this critical signaling network. Conclusion: Those findings indicated that CCDC7 may be a novel target for the treatment of cervical cancer and may have applications as a predictive marker for tumor growth in cervical carcinoma.

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“…Administration of chick embryo extract causes a considerable increase in the content of serum IL-1ß and IL-6, plays an important role in stimulation of hematopoiesis and provides a basis to consider this substance as an immunomodulator in pathologic immunosuppresive states [ 16 ]. Previous study illustrated that IL-6 cytokine induces angiogenesis [ 32 ]. The recently, more attentions have been given to the close relationship between tissue regeneration and angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Chicken Embryo Brain Extract on Sciatic Nerve Regeneration of Male Rat; An Experimental Study

Rasouli-Sangani

Farjah

Nasry

2018

BEAT

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Objective:To determine the effects of chicken embryo brain extract (BE) on transects sciatic nerve in male rats. Methods:Thirty adult male Sprague-Dawley rats weighing 200 to 250 g, were randomized into three groups treated with (1) sham surgery, (2) normal saline (NS), and (3) brain extract (BE). The BE was taken from incubating chick embryos at day 8. The sciatic nerve was exposed and sharply transected at the mid thigh level. Immediate epineurial repair was then performed. The BE treated animals were given 400 µl/kg of the chick embryo BE intraperitoneal, once daily, for 2 weeks. All animals were evaluated by sciatic functional index (SFI), electrophysiology, histology, and immunohistochemistry at days 28, 90 after surgery. Results:The mean SFI difference between BE and NS groups at days 28, 60 and 90 after surgery was statistically significant (p=0.086). The mean number of myelinated fibers in the BE group was significantly greater than that of the NS group on days 28 and 90 after surgery (p=0.034). At days 28 and 90 after surgery, the mean nerve conduction velocity (NCV) in the BE group was significantly faster than that of the NS group (p=0.041). Conclusion: These results indicate for the first time that chick embryo brain extract can enhance peripheral nerve regeneration in rat.

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Interleukin-6 secreted by oral cancer- associated fibroblast accelerated VEGF expression in tumor and stroma cells

Cited by 30 publications

References 31 publications

Anti‐angiogenic efficacy of aflibercept and bevacizumab in primary oral squamous cell carcinoma cells

Anti‐angiogenic efficacy of aflibercept and bevacizumab in primary oral squamous cell carcinoma cells

<p>CCDC7 Activates Interleukin-6 and Vascular Endothelial Growth Factor to Promote Proliferation via the JAK-STAT3 Pathway in Cervical Cancer Cells</p>

The Effects of Chicken Embryo Brain Extract on Sciatic Nerve Regeneration of Male Rat; An Experimental Study

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