Interleukin-6 secretion in vitro is up-regulated in ectopic and eutopic endometrial stromal cells from women with endometriosis.

Abstract

An in vitro model developed to compare human endometrial and endometriosis stromal cells was used to examine basal and stimulated expression of interleukin (IL-6). Stromal cells isolated from normal endometrium (NE) exhibited the lowest level of IL-6 secretion (84 pg/10(6) cells-48 h), whereas those cells isolated from endometriosis implants (EI) secreted the highest concentration of this inflammatory cytokine (46,284 pg/10(5) cells-48 h; P < 0.01). Eutopic endometrial stromal cells from women with endometriosis (EE) expressed an intermediate concentration of IL-6 (831 pg/10(6) cells-48 h). Stimulation of the various cultures with IL-1 beta dramatically augmented stromal cell production of IL-6. The mean concentrations of stimulated IL-6 secretion were 16,257, 37,800, and 264,290 pg/10(5) cells-48 h for NE, EE, and EI cells, respectively (P < 0.03). Exposure of the cell cultures to 10 nmol/L estradiol had little direct effect on IL-6 production. The results indicate that endometrial stromal cells isolated from tissues of women with and without endometriosis express IL-6 under basal and cytokine-stimulated conditions. Differential responsiveness among the three cell sources indicates that NE, EE, and EI cells have intrinsic quantitative differences in cytokine regulation.

Aberrant DNA methylation status of endometriosis: Epigenetics as the pathogenesis, biomarker and therapeutic target

“…These include a variety of anomalies in structure, proliferation, immune components, adhesion molecules, proteolytic enzymes and their inhibitors, steroid and cytokine production and responsiveness, gene expression and protein production. Such anomalies include, for example, the deficient expression of 17-hydroxysteroid dehydrogenase type 2, 6 interleukin (IL)-1 receptor type I 7 and homeobox (HOX) A10, 8 and enhanced expression of IL-6, 9 aromatase 10 and B-cell lymphoma/leukemia (BCL)-2, 11 in endometriosis. All of these differences may promote the development and progression of endometriosis.…”

Role of melatonin in regulating matrix metalloproteinase‐9 via tissue inhibitors of metalloproteinase‐1 during protection against endometriosis

“…It is estimated to affect 10-20% of women of reproductive age and affects quality of life negatively as it is associated with chronic pelvic pain, severe dysmenorrhea, dyspareunia and infertility [2][3][4]. The progression of endometriosis shows a strong correlation to estrogen levels and cytokines [5][6][7]. The etiology and pathogenesis of endometriosis are still unclear.…”

Overexpression of interleukin-6 in human basal cell carcinoma cell lines increases anti-apoptotic activity and tumorigenic potency

“…For example, synovial¯uids from patients with rheumatoid arthritis, a chronic in¯ammatory disease, contain high levels of IL-6 (Otake et al, 1996) that may be responsible for some of the systemic abnormalities common in arthritic patients. Similarly, IL-6 has been implicated in the pathogenesis of psoriatic plaque (Skelly et al, 1994;Tseng et al, 1996), which is an idiopathic skin disease that is histophathologically characterized by accelerated epidermal proliferation and in®ltration of in¯ammatory cells. In addition, has been shown to function as a growth factor in several human tumors, such as multiple myeloma, renal cell carcinoma, prostate cancer, Kaposi's sarcoma and colorectal cancer (Akira and Kishimoto, 1992b;Aoyagi et al, 1996;Adler et al, 1999;Aoki et al, 1999;Kinoshita et al, 1999).…”