Interleukin-8 production by the human cervix

Barclay, C.; Brennand, Janet; Kelly, Rodney W.; Galder, Andrew A.

doi:10.1016/0002-9378(93)90634-u

Cited by 147 publications

(60 citation statements)

References 20 publications

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“…Similar patterns of cytokines have been detected in cervical fluid recovered in vivo, with variations in abundance attributed to resident microflora and infection, and incidence of cigarette smoking (36,37). In situ localization studies identify epithelial cells as the principal cellular source of GM-CSF (38,39), 40), and IL-8 (41,42) in the cervix.…”

Section: Discussionmentioning

confidence: 61%

Seminal Fluid Induces Leukocyte Recruitment and Cytokine and Chemokine mRNA Expression in the Human Cervix after Coitus

Sharkey

Tremellen

Jasper

et al. 2012

The Journal of Immunology

297

312

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In mice, seminal fluid elicits an inflammation-like response in the female genital tract that activates immune adaptations to advance the likelihood of conception and pregnancy. In this study, we examined whether similar changes in leukocyte and cytokine parameters occur in the human cervix in response to the male partner’s seminal fluid. After a period of abstinence in proven-fertile women, duplicate sets of biopsies were taken from the ectocervix in the periovulatory period and again 48 h later, 12 h after unprotected vaginal coitus, vaginal coitus with use of a condom, or no coitus. A substantial influx of CD45+ cells mainly comprising CD14+ macrophages and CD1a+ dendritic cells expressing CD11a and MHC class II was evident in both the stratified epithelium and deeper stromal tissue after coitus. CD3+CD8+CD45RO+ T cells were also abundant and increased after coitus. Leukocyte recruitment did not occur without coitus or with condom-protected coitus. An accompanying increase in CSF2, IL6, IL8, and IL1A expression was detected by quantitative RT-PCR, and microarray analysis showed genes linked with inflammation, immune response, and related pathways are induced by seminal fluid in cervical tissues. We conclude that seminal fluid introduced at intercourse elicits expression of proinflammatory cytokines and chemokines, and a robust recruitment of macrophages, dendritic cells, and memory T cells. The leukocyte and cytokine environment induced in the cervix by seminal fluid appears competent to initiate adaptations in the female immune response that promote fertility. This response is also relevant to transmission of sexually transmitted pathogens and potentially, susceptibility to cervical metaplasia.

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Section: Discussionmentioning

confidence: 61%

Seminal Fluid Induces Leukocyte Recruitment and Cytokine and Chemokine mRNA Expression in the Human Cervix after Coitus

Sharkey

Tremellen

Jasper

et al. 2012

The Journal of Immunology

297

312

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“…Uterine cervical epithelial cells are now believed to play a central role in uterine CR by regulating multiple functions, cited earlier in the introduction (Barclay et al 1993, Pfundt et al 1996, Mahendroo et al 1999, Minjarez et al 2000, Word et al 2007. We have previously shown that exogenous VEGF administered to ovariectomized mice induces folding of the uterine cervical epithelia layer using SEM and interpreted these data to mean that VEGF induces proliferation of uterine cervical epithelial cells ).…”

Section: Discussionmentioning

confidence: 75%

“…A detailed understanding of uterine cervical epithelial biology has become essential because it is now believed that they play important and broad range of roles during uterine cervical remodeling (CR), which include i) maintaining fluid balance via synthesis of hydrophilic hyaluronan, glycosaminoglycan, and aquaporins; ii) proliferation and differentiation; iii) regulation of paracellular transport of solutes via tight junctions; iv) providing a protective barrier against invading microorganisms, and mediating inflammatory and adaptive immune responses; and v) acting as an 'endocrine' gland by synthesizing prostaglandins, chemokines, and cytokines (e.g. interleukin-8) and steroid hormones (Barclay et al 1993, Pfundt et al 1996, Mahendroo et al 1999, Minjarez et al 2000, Word et al 2007. It is likely, as some studies suggest that uterine cervical epithelial cells may play the central role in CR.…”

Section: Introductionmentioning

confidence: 99%

Vascular endothelial growth factor induces growth of uterine cervix and immune cell recruitment in mice

Donnelly¹,

Nguyen²,

Estes³

et al. 2013

Journal of Endocrinology

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“…macrophages, endothelial cells and even phagocytising PMNs (Bazzoni et al 1991). IL8 expression is downregulated during gestation by high progesterone (P 4 ) concentrations (Barclay et al 1993, Kelly et al 1994. In human placenta, IL8 production is increased after spontaneous labour and was localised in the perivascular area of foetal vessels in third-trimester placenta (Elliott et al 1998).…”

Section: Genes Related To Immune Responsementioning

confidence: 99%

Gene expression profiling of bovine peripartal placentomes: detection of molecular pathways potentially involved in the release of foetal membranes

Streyl¹,

Kenngott²,

Herbach³

et al. 2012

Reproduction

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The mechanisms underlying detachment of foetal membranes after birth in cows are still unclear. To address this problem in a systematic manner, we performed the first holistic transcriptome study of bovine placentomes antepartum (AP; nZ4 cows) and intrapartum (IP; nZ4 cows) using Affymetrix GeneChip Bovine Genome Arrays. Three placentomes were extracted from each cow, and tissue samples from the contact zones of the placentomes (foeto-maternal units) were recovered by systematic random sampling and processed for RNA extraction and for stereological quantification of cellular composition. Statistical analysis of microarray data (false discovery rate 1%) revealed 759 mRNAs with at least twofold higher levels in the samples of the AP group, whereas 514 mRNAs showed higher levels in the IP group. The differentially expressed genes were classified according to biological processes and molecular functions using the Functional Annotation Clustering tool of the DAVID Bioinformatics Resources. Genes with higher mRNA levels in the AP group were nearly completely related to mitotic cell cycle and tissue differentiation. During parturition, a complete shift occurred because the genes with higher mRNA levels in IP were nearly all related to three different physiological processes/complexes: i) apoptosis, ii) degradation of extra cellular matrix and iii) innate immune response, which play a fundamental role in placental detachment. These results are an excellent basis for future studies investigating the molecular basis of retained foetal membranes.

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Interleukin-8 production by the human cervix

Cited by 147 publications

References 20 publications

Seminal Fluid Induces Leukocyte Recruitment and Cytokine and Chemokine mRNA Expression in the Human Cervix after Coitus

Seminal Fluid Induces Leukocyte Recruitment and Cytokine and Chemokine mRNA Expression in the Human Cervix after Coitus

Vascular endothelial growth factor induces growth of uterine cervix and immune cell recruitment in mice

Gene expression profiling of bovine peripartal placentomes: detection of molecular pathways potentially involved in the release of foetal membranes

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