Interleukin-9 Promotes Pancreatic Cancer Cells Proliferation and Migration via the miR-200a/Beta-Catenin Axis

Abstract

Background. Both IL-9 and miR-200a are involved in the pathogenesis of cancers; however, the role of IL-9 in pancreatic cancer and the possible underlying mechanisms remain unknown. The aim of this study was to investigate the effect of IL-9 on pancreatic cancer cells and its interaction with miR-200a. Methods. Pancreatic cancer cells (PANC-1 and AsPC-1) were treated with IL-9 and the expression of miR-200a and β-catenin in pancreatic cancer cells was measured. β-Catenin was examined as a target gene of miR-200a in pancreatic cancer cells. The interaction between IL-9 and miR-200a in pancreatic cancer cells was determined by infecting miR-200a mimics prior to IL-9 treatment and then measuring miR-200a and β-catenin expression. Results. IL-9 significantly promoted the proliferation, invasion, and migration of pancreatic cancer cells; however, the effect on pancreatic cancer cell apoptosis was insignificant. β-Catenin was verified as a target gene of miR-200a in pancreatic cancer cells. Overexpression of miR-200a in pancreatic cancer cells significantly attenuated proliferation and metastasis and reduced β-catenin expression. IL-9 treatment of pancreatic cancer cells decreased miR-200a expression and increased β-catenin expression. The effect of miR-200a on pancreatic cancer cells decreased following IL-9 treatment. Conclusions. IL-9 promotes proliferation and metastasis in pancreatic cancer cells; this effect may partly involve regulation of the miR-200a/β-catenin axis.

Exploring the relationships among MiR-200a expression, hepatic fibrosis, and liver inflammation in biliary atresia mice model

“…Therefore, miR-200a is directly related with hepatic fibrosis. This finding is consistent with domestic and foreign research results [10].…”

Antibody-based delivery of Interleukin-9 to neovascular structures: therapeutic evaluation in cancer and arthritis

“…It has been generally suggested that IL9 may inhibit the growth of solid tumors by activating the innate or adaptive immune response [55,56], while as a lymphocyte growth factor it may promote progression of hematological malignancies [10,12]. However, since IL9 has also been reported to promote proliferation of multiple solid tumor models (like pancreatic cancer, lung cancer and colitis associated cancer) [57][58][59], its anti-cancer activity may be context and tumor type specific [52][53][54].…”

The emerging role of super enhancer-derived noncoding RNAs in human cancer

“…However, whether SE regulates apoptosis by regulating the expression of lncRNAs has not been further analyzed. SE-miR-200a is a proven epithelial-specific SE-derived ncRNA 8 , and miR-200a can inhibit cell apoptosis in many tumors 91 - 93 . Therefore, we speculate that SE-miR-200a is involved in the chemoresistance mechanism whereby apoptosis is inhibited in tumor cells.…”